Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs

Gliomas are a group of primary brain tumors characterized by their aggressive nature and resistance to treatment. Infiltration of surrounding normal tissues limits surgical approaches, wide inter- and intratumor heterogeneity hinders the development of universal therapeutics, and the presence of the...

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Main Authors: Daria Lanskikh, Olga Kuziakova, Ivan Baklanov, Alina Penkova, Veronica Doroshenko, Ivan Buriak, Valeriia Zhmenia, Vadim Kumeiko
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Cells
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Online Access:https://www.mdpi.com/2073-4409/13/24/2085
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author Daria Lanskikh
Olga Kuziakova
Ivan Baklanov
Alina Penkova
Veronica Doroshenko
Ivan Buriak
Valeriia Zhmenia
Vadim Kumeiko
author_facet Daria Lanskikh
Olga Kuziakova
Ivan Baklanov
Alina Penkova
Veronica Doroshenko
Ivan Buriak
Valeriia Zhmenia
Vadim Kumeiko
author_sort Daria Lanskikh
collection DOAJ
description Gliomas are a group of primary brain tumors characterized by their aggressive nature and resistance to treatment. Infiltration of surrounding normal tissues limits surgical approaches, wide inter- and intratumor heterogeneity hinders the development of universal therapeutics, and the presence of the blood–brain barrier reduces the efficiency of their delivery. As a result, patients diagnosed with gliomas often face a poor prognosis and low survival rates. The spectrum of anti-glioma drugs used in clinical practice is quite narrow. Alkylating agents are often used as first-line therapy, but their effectiveness varies depending on the molecular subtypes of gliomas. This highlights the need for new, more effective therapeutic approaches. Standard drug-screening methods involve the use of two-dimensional cell cultures. However, these models cannot fully replicate the conditions present in real tumors, making it difficult to extrapolate the results to humans. We describe the advantages and disadvantages of existing glioma cell-based models designed to improve the situation and build future prospects to make drug discovery comprehensive and more effective for each patient according to personalized therapy paradigms.
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institution Kabale University
issn 2073-4409
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publishDate 2024-12-01
publisher MDPI AG
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series Cells
spelling doaj-art-7802f480ca554047925bd366d67dcbc42024-12-27T14:16:33ZengMDPI AGCells2073-44092024-12-011324208510.3390/cells13242085Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional ConstructsDaria Lanskikh0Olga Kuziakova1Ivan Baklanov2Alina Penkova3Veronica Doroshenko4Ivan Buriak5Valeriia Zhmenia6Vadim Kumeiko7School of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaSchool of Medicine and Life Sciences, Far Eastern Federal University, 690922 Vladivostok, RussiaGliomas are a group of primary brain tumors characterized by their aggressive nature and resistance to treatment. Infiltration of surrounding normal tissues limits surgical approaches, wide inter- and intratumor heterogeneity hinders the development of universal therapeutics, and the presence of the blood–brain barrier reduces the efficiency of their delivery. As a result, patients diagnosed with gliomas often face a poor prognosis and low survival rates. The spectrum of anti-glioma drugs used in clinical practice is quite narrow. Alkylating agents are often used as first-line therapy, but their effectiveness varies depending on the molecular subtypes of gliomas. This highlights the need for new, more effective therapeutic approaches. Standard drug-screening methods involve the use of two-dimensional cell cultures. However, these models cannot fully replicate the conditions present in real tumors, making it difficult to extrapolate the results to humans. We describe the advantages and disadvantages of existing glioma cell-based models designed to improve the situation and build future prospects to make drug discovery comprehensive and more effective for each patient according to personalized therapy paradigms.https://www.mdpi.com/2073-4409/13/24/2085gliomathree-dimensional modelbrain tumorscancergenetically modified modelcell-based screening
spellingShingle Daria Lanskikh
Olga Kuziakova
Ivan Baklanov
Alina Penkova
Veronica Doroshenko
Ivan Buriak
Valeriia Zhmenia
Vadim Kumeiko
Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
Cells
glioma
three-dimensional model
brain tumors
cancer
genetically modified model
cell-based screening
title Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
title_full Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
title_fullStr Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
title_full_unstemmed Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
title_short Cell-Based Glioma Models for Anticancer Drug Screening: From Conventional Adherent Cell Cultures to Tumor-Specific Three-Dimensional Constructs
title_sort cell based glioma models for anticancer drug screening from conventional adherent cell cultures to tumor specific three dimensional constructs
topic glioma
three-dimensional model
brain tumors
cancer
genetically modified model
cell-based screening
url https://www.mdpi.com/2073-4409/13/24/2085
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