Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights
Heat shock proteins (HSP) have been associated with a range of persistent inflammatory disorders; however, little research has been conducted on the involvement of HSP in the development of ankylosing spondylitis (AS). The research aims to identify a diagnostic signature based on HSP-related genes a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | International Journal of Hyperthermia |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/02656736.2024.2336149 |
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| author | Geqiang Wang Yongji Li Jiaxing Liu Qian Zhang Weixin Cai Xiaodong Li |
| author_facet | Geqiang Wang Yongji Li Jiaxing Liu Qian Zhang Weixin Cai Xiaodong Li |
| author_sort | Geqiang Wang |
| collection | DOAJ |
| description | Heat shock proteins (HSP) have been associated with a range of persistent inflammatory disorders; however, little research has been conducted on the involvement of HSP in the development of ankylosing spondylitis (AS). The research aims to identify a diagnostic signature based on HSP-related genes and determine the molecular subtypes of AS. We gathered the transcriptional data of patients with AS from the GSE73754 dataset and conducted a literature search for HSP-related genes (HRGs). The logistic regression model was utilized for the identification of hub HRGs associated with AS. Subsequently, these HRGs were employed in the construction of a nomogram prediction model. We employed a consensus clustering approach to identify novel molecular subgroups. Subsequently, we conducted functional analyses, encompassing GO, KEGG, and GSEA, to elucidate the underlying mechanisms between these subgroups. To assess the immunological landscape, we employed the xCell algorithm. Through logistic regression analysis, the four core HRGs (CCT2, HSPA6, DNAJB14, and DNAJC5) were confirmed as potential biomarkers for AS. Subsequent stratification revealed two distinct molecular phenotypes, designated as Cluster 1 and Cluster 2. Notably, Cluster 2 was characterized by the upregulation of pathways pertinent to immune response and inflammation. Our research suggests that the CCT2, HSPA6, DNAJB14, and DNAJC5 exhibit potential as effective blood-based diagnostic biomarkers for AS. These findings contribute to a deeper comprehension of the underlying mechanisms involved in the development of AS and offer potential targets for personalized therapeutic interventions. |
| format | Article |
| id | doaj-art-77ee7307c5a1441ab699c87ecaabd55e |
| institution | Kabale University |
| issn | 0265-6736 1464-5157 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Hyperthermia |
| spelling | doaj-art-77ee7307c5a1441ab699c87ecaabd55e2025-01-03T09:30:27ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572024-12-0141110.1080/02656736.2024.2336149Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insightsGeqiang Wang0Yongji Li1Jiaxing Liu2Qian Zhang3Weixin Cai4Xiaodong Li5Department of Orthopaedics and Traumatology III, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaDepartment of Orthopaedics and Traumatology I, The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaDepartment of Orthopaedics and Traumatology III, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaDepartment of Orthopaedics and Traumatology III, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaDepartment of Orthopaedics and Traumatology III, The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaDepartment of Orthopaedics, The Third Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine, ChinaHeat shock proteins (HSP) have been associated with a range of persistent inflammatory disorders; however, little research has been conducted on the involvement of HSP in the development of ankylosing spondylitis (AS). The research aims to identify a diagnostic signature based on HSP-related genes and determine the molecular subtypes of AS. We gathered the transcriptional data of patients with AS from the GSE73754 dataset and conducted a literature search for HSP-related genes (HRGs). The logistic regression model was utilized for the identification of hub HRGs associated with AS. Subsequently, these HRGs were employed in the construction of a nomogram prediction model. We employed a consensus clustering approach to identify novel molecular subgroups. Subsequently, we conducted functional analyses, encompassing GO, KEGG, and GSEA, to elucidate the underlying mechanisms between these subgroups. To assess the immunological landscape, we employed the xCell algorithm. Through logistic regression analysis, the four core HRGs (CCT2, HSPA6, DNAJB14, and DNAJC5) were confirmed as potential biomarkers for AS. Subsequent stratification revealed two distinct molecular phenotypes, designated as Cluster 1 and Cluster 2. Notably, Cluster 2 was characterized by the upregulation of pathways pertinent to immune response and inflammation. Our research suggests that the CCT2, HSPA6, DNAJB14, and DNAJC5 exhibit potential as effective blood-based diagnostic biomarkers for AS. These findings contribute to a deeper comprehension of the underlying mechanisms involved in the development of AS and offer potential targets for personalized therapeutic interventions.https://www.tandfonline.com/doi/10.1080/02656736.2024.2336149Ankylosing spondylitisdiagnostic biomarkerssubtypesheat shock proteinimmune infiltration |
| spellingShingle | Geqiang Wang Yongji Li Jiaxing Liu Qian Zhang Weixin Cai Xiaodong Li Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights International Journal of Hyperthermia Ankylosing spondylitis diagnostic biomarkers subtypes heat shock protein immune infiltration |
| title | Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights |
| title_full | Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights |
| title_fullStr | Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights |
| title_full_unstemmed | Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights |
| title_short | Heat shock protein-related diagnostic signature and molecular subtypes in ankylosing spondylitis: new pathogenesis insights |
| title_sort | heat shock protein related diagnostic signature and molecular subtypes in ankylosing spondylitis new pathogenesis insights |
| topic | Ankylosing spondylitis diagnostic biomarkers subtypes heat shock protein immune infiltration |
| url | https://www.tandfonline.com/doi/10.1080/02656736.2024.2336149 |
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