Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer

Abstract Cancer cells adapt their metabolism to support aberrant cell proliferation. However, the functional link between metabolic reprogramming and cell cycle progression remains largely unexplored. Mitochondria rely on the transfer of multiple lipids from the endoplasmic reticulum (ER) to their m...

Full description

Saved in:
Bibliographic Details
Main Authors: Ewelina Dondajewska, Paula Allepuz‐Fuster, Chloé Maurizy, Alexandre Hego, Sandra Ormenese, Quentin Lion, Arnaud Blomme, Pierre Close, Arnaud Lavergne, Latifa Karim, Marc Thiry, Ivan Nemazanyy, Roopesh Krishnankutty, Jair Marques Jr, Alex vonKriegsheim, Nathaniel F. Henneman, Ganna Panasyuk, Kateryna Shostak, Alain Chariot
Format: Article
Language:English
Published: Wiley 2025-08-01
Series:Advanced Science
Subjects:
Online Access:https://doi.org/10.1002/advs.202503022
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849227302190186496
author Ewelina Dondajewska
Paula Allepuz‐Fuster
Chloé Maurizy
Alexandre Hego
Sandra Ormenese
Quentin Lion
Arnaud Blomme
Pierre Close
Arnaud Lavergne
Latifa Karim
Marc Thiry
Ivan Nemazanyy
Roopesh Krishnankutty
Jair Marques Jr
Alex vonKriegsheim
Nathaniel F. Henneman
Ganna Panasyuk
Kateryna Shostak
Alain Chariot
author_facet Ewelina Dondajewska
Paula Allepuz‐Fuster
Chloé Maurizy
Alexandre Hego
Sandra Ormenese
Quentin Lion
Arnaud Blomme
Pierre Close
Arnaud Lavergne
Latifa Karim
Marc Thiry
Ivan Nemazanyy
Roopesh Krishnankutty
Jair Marques Jr
Alex vonKriegsheim
Nathaniel F. Henneman
Ganna Panasyuk
Kateryna Shostak
Alain Chariot
author_sort Ewelina Dondajewska
collection DOAJ
description Abstract Cancer cells adapt their metabolism to support aberrant cell proliferation. However, the functional link between metabolic reprogramming and cell cycle progression remains largely unexplored. Mitochondria rely on the transfer of multiple lipids from the endoplasmic reticulum (ER) to their membranes to be functional. Several mitochondrial‐derived metabolites influence cancer cell proliferation by modulating the epigenome. Here, the loss of STARD7, a lipid transfer protein whose expression is enhanced in breast cancer, is shown to lead a metabolic reprogramming characterized by the accumulation of carnitine derivatives and S‐Adenosyl‐L‐methionine (SAM). Elevated SAM levels cause the increase of H3K27 trimethylation on many gene promoters coding for candidates involved in cell cycle progression. Likewise, STARD7 deficiency triggers cell cycle arrest and impairs ERα‐dependent cell proliferation. Moreover, EGFR signaling is also impaired in triple negative breast cancer cells lacking STARD7, at least due to deregulated EGFR trafficking to lysosomes. Therefore, mitochondria rely on STARD7 to support cell cycle progression in breast cancer.
format Article
id doaj-art-76fe231cf8b74ce6aaa963d9535873bf
institution Kabale University
issn 2198-3844
language English
publishDate 2025-08-01
publisher Wiley
record_format Article
series Advanced Science
spelling doaj-art-76fe231cf8b74ce6aaa963d9535873bf2025-08-23T14:12:31ZengWileyAdvanced Science2198-38442025-08-011231n/an/a10.1002/advs.202503022Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast CancerEwelina Dondajewska0Paula Allepuz‐Fuster1Chloé Maurizy2Alexandre Hego3Sandra Ormenese4Quentin Lion5Arnaud Blomme6Pierre Close7Arnaud Lavergne8Latifa Karim9Marc Thiry10Ivan Nemazanyy11Roopesh Krishnankutty12Jair Marques Jr13Alex vonKriegsheim14Nathaniel F. Henneman15Ganna Panasyuk16Kateryna Shostak17Alain Chariot18Laboratory of Cancer Biology Liège BelgiumLaboratory of Cancer Biology Liège BelgiumLaboratory of Cancer Biology Liège BelgiumGIGA Flow Cytometry and Cell Imaging Platform University of Liege Liege BelgiumGIGA Flow Cytometry and Cell Imaging Platform University of Liege Liege BelgiumLaboratory of Cancer Biology Liège BelgiumGIGA Cancer GIGA University of Liege CHU Sart‐Tilman Liège 4000 BelgiumGIGA Cancer GIGA University of Liege CHU Sart‐Tilman Liège 4000 BelgiumGIGA Genomics Platform University of Liege Liege BelgiumGIGA Genomics Platform University of Liege Liege BelgiumUnit of Cell and Tissue Biology GIGA Neurosciences University of Liege Liege BelgiumPlatform for Metabolic Analyses Structure Fédérative de Recherche Necker INSERM US24/CNRS UAR 3633 Paris 75015 FranceInstitute of Genetics and Cancer Edinburgh Cancer Research ScotlandInstitute of Genetics and Cancer Edinburgh Cancer Research ScotlandInstitute of Genetics and Cancer Edinburgh Cancer Research ScotlandInstitut Necker‐Enfants Malades (INEM) INSERM U1151/CNRS UMR 8253, 75015 Paris France and Université de Paris Cité Paris 75006 FranceInstitut Necker‐Enfants Malades (INEM) INSERM U1151/CNRS UMR 8253, 75015 Paris France and Université de Paris Cité Paris 75006 FranceLaboratory of Cancer Biology Liège BelgiumLaboratory of Cancer Biology Liège BelgiumAbstract Cancer cells adapt their metabolism to support aberrant cell proliferation. However, the functional link between metabolic reprogramming and cell cycle progression remains largely unexplored. Mitochondria rely on the transfer of multiple lipids from the endoplasmic reticulum (ER) to their membranes to be functional. Several mitochondrial‐derived metabolites influence cancer cell proliferation by modulating the epigenome. Here, the loss of STARD7, a lipid transfer protein whose expression is enhanced in breast cancer, is shown to lead a metabolic reprogramming characterized by the accumulation of carnitine derivatives and S‐Adenosyl‐L‐methionine (SAM). Elevated SAM levels cause the increase of H3K27 trimethylation on many gene promoters coding for candidates involved in cell cycle progression. Likewise, STARD7 deficiency triggers cell cycle arrest and impairs ERα‐dependent cell proliferation. Moreover, EGFR signaling is also impaired in triple negative breast cancer cells lacking STARD7, at least due to deregulated EGFR trafficking to lysosomes. Therefore, mitochondria rely on STARD7 to support cell cycle progression in breast cancer.https://doi.org/10.1002/advs.202503022breast cancercell cycle arrestEGFR signalinglipid transfer proteinmetabolic reprogramming
spellingShingle Ewelina Dondajewska
Paula Allepuz‐Fuster
Chloé Maurizy
Alexandre Hego
Sandra Ormenese
Quentin Lion
Arnaud Blomme
Pierre Close
Arnaud Lavergne
Latifa Karim
Marc Thiry
Ivan Nemazanyy
Roopesh Krishnankutty
Jair Marques Jr
Alex vonKriegsheim
Nathaniel F. Henneman
Ganna Panasyuk
Kateryna Shostak
Alain Chariot
Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
Advanced Science
breast cancer
cell cycle arrest
EGFR signaling
lipid transfer protein
metabolic reprogramming
title Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
title_full Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
title_fullStr Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
title_full_unstemmed Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
title_short Loss of STARD7 Triggers Metabolic Reprogramming and Cell Cycle Arrest in Breast Cancer
title_sort loss of stard7 triggers metabolic reprogramming and cell cycle arrest in breast cancer
topic breast cancer
cell cycle arrest
EGFR signaling
lipid transfer protein
metabolic reprogramming
url https://doi.org/10.1002/advs.202503022
work_keys_str_mv AT ewelinadondajewska lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT paulaallepuzfuster lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT chloemaurizy lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT alexandrehego lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT sandraormenese lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT quentinlion lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT arnaudblomme lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT pierreclose lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT arnaudlavergne lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT latifakarim lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT marcthiry lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT ivannemazanyy lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT roopeshkrishnankutty lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT jairmarquesjr lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT alexvonkriegsheim lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT nathanielfhenneman lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT gannapanasyuk lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT katerynashostak lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer
AT alainchariot lossofstard7triggersmetabolicreprogrammingandcellcyclearrestinbreastcancer