Investigating dutasteride fluorescence for highly sensitive analytical profit
Abstract For the first time, an extremely rapid and sensitive simple spectrofluorometric method was developed based on measuring the native fluorescence of dutasteride (DUT) in acetonitrile. Following its excitation at 245 nm, the intensity of the fluorescence was measured at 325 nm. The linearity l...
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| Format: | Article |
| Language: | English |
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Springer
2025-05-01
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| Series: | Discover Chemistry |
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| Online Access: | https://doi.org/10.1007/s44371-025-00192-1 |
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| author | Mariam Rashad Fawzi Elsebaei Zeinab Sheribah Mohamed Walash |
| author_facet | Mariam Rashad Fawzi Elsebaei Zeinab Sheribah Mohamed Walash |
| author_sort | Mariam Rashad |
| collection | DOAJ |
| description | Abstract For the first time, an extremely rapid and sensitive simple spectrofluorometric method was developed based on measuring the native fluorescence of dutasteride (DUT) in acetonitrile. Following its excitation at 245 nm, the intensity of the fluorescence was measured at 325 nm. The linearity lies in the range of 20.0–600.0 ng mL−1. The developed method was optimized and validated in compliance with ICH guidelines. This abstract follows reviewer recommendations. For the first time, an extremely rapid, eco-friendly, cost-effective, accurate, sensitive, and simple spectrofluorometric method was developed based on measuring the native fluorescence of dutasteride (DUT) used in the treatment of benign prostatic hyperplasia (BPH) in acetonitrile (ACN) as the diluting solvent. Following its excitation at 245 nm, the intensity of the fluorescence emission was measured at 325 nm. The linearity lies in the range of 20.0–600.0 ng mL⁻1 without the use of any surfactant or buffer at room temperature. It was also applied on a prepared tablet. The developed method was optimized and validated in compliance with ICH guidelines. Dutasteride (DUT) estimation using the suggested approach was found to be precise, accurate, robust, specific, and sensitive. In addition, we used some of the greenness assessment and environmental safety methods, such as AGREE and Analytical Eco Scale, to prove its ecological capability in terms of solvent, chemical, energy consumption, and waste generation. |
| format | Article |
| id | doaj-art-767b51e25d8a4f55bbf61f813af6c10c |
| institution | DOAJ |
| issn | 3005-1193 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Chemistry |
| spelling | doaj-art-767b51e25d8a4f55bbf61f813af6c10c2025-08-20T03:09:19ZengSpringerDiscover Chemistry3005-11932025-05-012111010.1007/s44371-025-00192-1Investigating dutasteride fluorescence for highly sensitive analytical profitMariam Rashad0Fawzi Elsebaei1Zeinab Sheribah2Mohamed Walash3Faculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura UniversityFaculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura UniversityFaculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura UniversityFaculty of Pharmacy, Pharmaceutical Analytical Chemistry Department, Mansoura UniversityAbstract For the first time, an extremely rapid and sensitive simple spectrofluorometric method was developed based on measuring the native fluorescence of dutasteride (DUT) in acetonitrile. Following its excitation at 245 nm, the intensity of the fluorescence was measured at 325 nm. The linearity lies in the range of 20.0–600.0 ng mL−1. The developed method was optimized and validated in compliance with ICH guidelines. This abstract follows reviewer recommendations. For the first time, an extremely rapid, eco-friendly, cost-effective, accurate, sensitive, and simple spectrofluorometric method was developed based on measuring the native fluorescence of dutasteride (DUT) used in the treatment of benign prostatic hyperplasia (BPH) in acetonitrile (ACN) as the diluting solvent. Following its excitation at 245 nm, the intensity of the fluorescence emission was measured at 325 nm. The linearity lies in the range of 20.0–600.0 ng mL⁻1 without the use of any surfactant or buffer at room temperature. It was also applied on a prepared tablet. The developed method was optimized and validated in compliance with ICH guidelines. Dutasteride (DUT) estimation using the suggested approach was found to be precise, accurate, robust, specific, and sensitive. In addition, we used some of the greenness assessment and environmental safety methods, such as AGREE and Analytical Eco Scale, to prove its ecological capability in terms of solvent, chemical, energy consumption, and waste generation.https://doi.org/10.1007/s44371-025-00192-1DutasterideFluorescenceBenign prostatic hyperplasiaPharmaceuticalPrepared tabletNative |
| spellingShingle | Mariam Rashad Fawzi Elsebaei Zeinab Sheribah Mohamed Walash Investigating dutasteride fluorescence for highly sensitive analytical profit Discover Chemistry Dutasteride Fluorescence Benign prostatic hyperplasia Pharmaceutical Prepared tablet Native |
| title | Investigating dutasteride fluorescence for highly sensitive analytical profit |
| title_full | Investigating dutasteride fluorescence for highly sensitive analytical profit |
| title_fullStr | Investigating dutasteride fluorescence for highly sensitive analytical profit |
| title_full_unstemmed | Investigating dutasteride fluorescence for highly sensitive analytical profit |
| title_short | Investigating dutasteride fluorescence for highly sensitive analytical profit |
| title_sort | investigating dutasteride fluorescence for highly sensitive analytical profit |
| topic | Dutasteride Fluorescence Benign prostatic hyperplasia Pharmaceutical Prepared tablet Native |
| url | https://doi.org/10.1007/s44371-025-00192-1 |
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