Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin
Summary: Here, we present a protocol for staining murine skin innervation by either a pan-axonal marker or a genetic tracer of sensory neuron subtypes using floating sections. We also describe steps for using a new MATLAB-based semiautomated routine that facilitates the quantification of innervation...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724004775 |
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| author | Swagata Dey Andrew Kovalenko Avraham Yaron |
| author_facet | Swagata Dey Andrew Kovalenko Avraham Yaron |
| author_sort | Swagata Dey |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for staining murine skin innervation by either a pan-axonal marker or a genetic tracer of sensory neuron subtypes using floating sections. We also describe steps for using a new MATLAB-based semiautomated routine that facilitates the quantification of innervation density. This protocol can also be applied to other organs, such as the mouse’s spinal cord and tongue.For complete details on the use and execution of this protocol, please refer to Dey et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-763b840e83c44e8ebc4328c0484f5ca1 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-763b840e83c44e8ebc4328c0484f5ca12024-11-20T05:07:46ZengElsevierSTAR Protocols2666-16672024-12-0154103312Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skinSwagata Dey0Andrew Kovalenko1Avraham Yaron2Department of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel; Corresponding authorDepartment of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Biomolecular Sciences and Molecular Neuroscience, Weizmann Institute of Science, Rehovot 76100, Israel; Corresponding authorSummary: Here, we present a protocol for staining murine skin innervation by either a pan-axonal marker or a genetic tracer of sensory neuron subtypes using floating sections. We also describe steps for using a new MATLAB-based semiautomated routine that facilitates the quantification of innervation density. This protocol can also be applied to other organs, such as the mouse’s spinal cord and tongue.For complete details on the use and execution of this protocol, please refer to Dey et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724004775Developmental biologyMicroscopyNeuroscience |
| spellingShingle | Swagata Dey Andrew Kovalenko Avraham Yaron Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin STAR Protocols Developmental biology Microscopy Neuroscience |
| title | Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| title_full | Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| title_fullStr | Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| title_full_unstemmed | Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| title_short | Protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| title_sort | protocol for semiautomated analysis of sensory neuronal innervation of the mouse footpad skin |
| topic | Developmental biology Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724004775 |
| work_keys_str_mv | AT swagatadey protocolforsemiautomatedanalysisofsensoryneuronalinnervationofthemousefootpadskin AT andrewkovalenko protocolforsemiautomatedanalysisofsensoryneuronalinnervationofthemousefootpadskin AT avrahamyaron protocolforsemiautomatedanalysisofsensoryneuronalinnervationofthemousefootpadskin |