Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4
Objective: We investigated a rapid detection method for carbapenemase-producing gram-negative bacilli (CP-GNR) using meropenem (MEPM) to assess the efficiency of the antimicrobial susceptibility testing. Methods: We used the function that can monitor the growth curve with the resistant bacteria moni...
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| Format: | Article |
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Elsevier
2024-12-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524001760 |
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| author | Yumiko Funashima Rin Hamabe Kei Tominaga Kentaro Wakamatsu Takahiro Yaguchi Zenzo Nagasawa Tsukuru Umemura |
| author_facet | Yumiko Funashima Rin Hamabe Kei Tominaga Kentaro Wakamatsu Takahiro Yaguchi Zenzo Nagasawa Tsukuru Umemura |
| author_sort | Yumiko Funashima |
| collection | DOAJ |
| description | Objective: We investigated a rapid detection method for carbapenemase-producing gram-negative bacilli (CP-GNR) using meropenem (MEPM) to assess the efficiency of the antimicrobial susceptibility testing. Methods: We used the function that can monitor the growth curve with the resistant bacteria monitoring function (RAISUS S4). Rapid detection of CP-GNR was performed using RAISUS S4 in two types of antimicrobial susceptibility testing, the RAISUS 18-hour method (18-h method) and RAISUS rapid method (rapid method) for Enterobacterales (F-GNR) and non-fermenting gram-negative bacilli (NF-GNR). Results: When F-GNR were based on MEPM MIC ≥ 0.25 µg/mL, CP-GNR were detected with a sensitivity of 100% (58/58) for the 18-h method and 98.3% (57/58) for the rapid method; the shortest detection times were 5.3 and 4.0 h, respectively. When NF-GNR were based on MEPM MIC > 8 µg/mL, it was possible to detect CP-GNR with 100% sensitivity (58/58) in both methods. Furthermore, in the analysis using the 18-h method for monitoring resistant bacteria, when ≥ 2 µg/mL was used as the screening concentration for F-GNR, approximately 50% of the resistant genotypes, NDM, GES, and KPC, were detected in approximately 7 h However, detecting the IMP and VIM took 11–12 h Conclusions: The 18-h and rapid methods with RAISUS S4 were highly correlated with the results of the microdilution method of CLSI, and CP-GNR detection was rapid using a function that can monitor the growth curve with RAISUS S4. |
| format | Article |
| id | doaj-art-7616f50c6e3744aab2a73b4c65803f04 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-7616f50c6e3744aab2a73b4c65803f042024-12-22T05:28:03ZengElsevierJournal of Global Antimicrobial Resistance2213-71652024-12-0139202208Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4Yumiko Funashima0Rin Hamabe1Kei Tominaga2Kentaro Wakamatsu3Takahiro Yaguchi4Zenzo Nagasawa5Tsukuru Umemura6Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare, Okawa, Fukuoka, Japan; Medical Laboratory Science Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School, Okawa, Fukuoka, JapanResearch Division, Shimadzu Diagnostics Corporation, Yuki, Ibaraki, JapanResearch Division, Shimadzu Diagnostics Corporation, Yuki, Ibaraki, JapanDepartment of Respiratory Medicine, National Hospital Organization Omuta National Hospital, Omuta, Fukuoka, JapanDepartment of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare, Okawa, Fukuoka, Japan; Medical Laboratory Science Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School, Okawa, Fukuoka, Japan; Corresponding author: Department of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare, 137-1 Enokizu, Okawa, Fukuoka, 831-8501, JapanDepartment of Medical Technology and Science, Faculty of Fukuoka Health Care, International University of Health and Welfare, Okawa, Fukuoka, Japan; Medical Laboratory Science Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School, Okawa, Fukuoka, JapanMedical Laboratory Science Graduate School of Health and Welfare Sciences, International University of Health and Welfare Graduate School, Okawa, Fukuoka, Japan; Department of Clinical Laboratory, Okawa, Fukuoka, JapanObjective: We investigated a rapid detection method for carbapenemase-producing gram-negative bacilli (CP-GNR) using meropenem (MEPM) to assess the efficiency of the antimicrobial susceptibility testing. Methods: We used the function that can monitor the growth curve with the resistant bacteria monitoring function (RAISUS S4). Rapid detection of CP-GNR was performed using RAISUS S4 in two types of antimicrobial susceptibility testing, the RAISUS 18-hour method (18-h method) and RAISUS rapid method (rapid method) for Enterobacterales (F-GNR) and non-fermenting gram-negative bacilli (NF-GNR). Results: When F-GNR were based on MEPM MIC ≥ 0.25 µg/mL, CP-GNR were detected with a sensitivity of 100% (58/58) for the 18-h method and 98.3% (57/58) for the rapid method; the shortest detection times were 5.3 and 4.0 h, respectively. When NF-GNR were based on MEPM MIC > 8 µg/mL, it was possible to detect CP-GNR with 100% sensitivity (58/58) in both methods. Furthermore, in the analysis using the 18-h method for monitoring resistant bacteria, when ≥ 2 µg/mL was used as the screening concentration for F-GNR, approximately 50% of the resistant genotypes, NDM, GES, and KPC, were detected in approximately 7 h However, detecting the IMP and VIM took 11–12 h Conclusions: The 18-h and rapid methods with RAISUS S4 were highly correlated with the results of the microdilution method of CLSI, and CP-GNR detection was rapid using a function that can monitor the growth curve with RAISUS S4.http://www.sciencedirect.com/science/article/pii/S2213716524001760Antimicrobial susceptibility testingCarbapenemase-producing enterobacteralesCarbapenem-resistant non-fermenting gram-negative bacilliIMPRAISUS S4 |
| spellingShingle | Yumiko Funashima Rin Hamabe Kei Tominaga Kentaro Wakamatsu Takahiro Yaguchi Zenzo Nagasawa Tsukuru Umemura Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 Journal of Global Antimicrobial Resistance Antimicrobial susceptibility testing Carbapenemase-producing enterobacterales Carbapenem-resistant non-fermenting gram-negative bacilli IMP RAISUS S4 |
| title | Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 |
| title_full | Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 |
| title_fullStr | Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 |
| title_full_unstemmed | Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 |
| title_short | Detection of carbapenem-resistant gram-negative bacilli in Japan using the fully automated bacterial testing device RAISUS S4 |
| title_sort | detection of carbapenem resistant gram negative bacilli in japan using the fully automated bacterial testing device raisus s4 |
| topic | Antimicrobial susceptibility testing Carbapenemase-producing enterobacterales Carbapenem-resistant non-fermenting gram-negative bacilli IMP RAISUS S4 |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524001760 |
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