Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration.
Bacterial cellulose (BC) is a novel biocompatible polymeric biomaterial with a wide range of biomedical uses, like tissue engineering (TE) scaffolds, wound dressings, and drug delivery. Although BC lacks good cell adhesion due to limited functionality, its tunable surface chemistry still holds promi...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0312396 |
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| author | Rabiu Salihu Saiful Izwan Abd Razak Mohd Helmi Sani Mohammed Ahmad Wsoo Nurliyana Ahmad Zawawi Shafinaz Shahir |
| author_facet | Rabiu Salihu Saiful Izwan Abd Razak Mohd Helmi Sani Mohammed Ahmad Wsoo Nurliyana Ahmad Zawawi Shafinaz Shahir |
| author_sort | Rabiu Salihu |
| collection | DOAJ |
| description | Bacterial cellulose (BC) is a novel biocompatible polymeric biomaterial with a wide range of biomedical uses, like tissue engineering (TE) scaffolds, wound dressings, and drug delivery. Although BC lacks good cell adhesion due to limited functionality, its tunable surface chemistry still holds promise. Here, hydroxyapatite (HA) was incorporated into a citrate-modified BC (MBC) using the biomimetic synthesis in simulated body fluid (SBF). Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), thermal gravimetric analysis (TGA), and compressive modulus were used to characterize the biomineralized MBC (BMBC) samples. Using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium (MTS), trypan blue dye exclusion (TBDE), and cell attachment assays on osteoblast cells, the developed BMBC have shown good cell viability, proliferation, and attachment after 3, 5, and 7 days of culture and therefore suggested as potential bone tissue regeneration scaffolding material. |
| format | Article |
| id | doaj-art-76000ad4c3494f9391d3049502c541df |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-76000ad4c3494f9391d3049502c541df2025-01-08T05:32:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031239610.1371/journal.pone.0312396Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration.Rabiu SalihuSaiful Izwan Abd RazakMohd Helmi SaniMohammed Ahmad WsooNurliyana Ahmad ZawawiShafinaz ShahirBacterial cellulose (BC) is a novel biocompatible polymeric biomaterial with a wide range of biomedical uses, like tissue engineering (TE) scaffolds, wound dressings, and drug delivery. Although BC lacks good cell adhesion due to limited functionality, its tunable surface chemistry still holds promise. Here, hydroxyapatite (HA) was incorporated into a citrate-modified BC (MBC) using the biomimetic synthesis in simulated body fluid (SBF). Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field emission scanning electron microscopy (FE-SEM), thermal gravimetric analysis (TGA), and compressive modulus were used to characterize the biomineralized MBC (BMBC) samples. Using 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl) -2H-tetrazolium (MTS), trypan blue dye exclusion (TBDE), and cell attachment assays on osteoblast cells, the developed BMBC have shown good cell viability, proliferation, and attachment after 3, 5, and 7 days of culture and therefore suggested as potential bone tissue regeneration scaffolding material.https://doi.org/10.1371/journal.pone.0312396 |
| spellingShingle | Rabiu Salihu Saiful Izwan Abd Razak Mohd Helmi Sani Mohammed Ahmad Wsoo Nurliyana Ahmad Zawawi Shafinaz Shahir Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. PLoS ONE |
| title | Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. |
| title_full | Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. |
| title_fullStr | Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. |
| title_full_unstemmed | Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. |
| title_short | Citrate-modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration. |
| title_sort | citrate modified bacterial cellulose as a potential scaffolding material for bone tissue regeneration |
| url | https://doi.org/10.1371/journal.pone.0312396 |
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