Protocol for closed-loop optogenetic manipulation of cortical activity following hippocampal sharp-wave ripples in freely behaving rats
Summary: Temporally and spatially selective closed-loop perturbation of brain activity can be used to reveal the contribution of spontaneous neural events to behavior. Here, we present a protocol for optogenetic inhibition of the medial prefrontal cortex following hippocampal sharp-wave ripple event...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003041 |
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| Summary: | Summary: Temporally and spatially selective closed-loop perturbation of brain activity can be used to reveal the contribution of spontaneous neural events to behavior. Here, we present a protocol for optogenetic inhibition of the medial prefrontal cortex following hippocampal sharp-wave ripple events in freely behaving rats. We describe procedures for injecting a viral vector bilaterally, constructing and implanting an assembly of optical fibers and tetrodes, and performing closed-loop perturbations in rats.For complete details on the use and execution of this protocol, please refer to den Bakker et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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| ISSN: | 2666-1667 |