Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis
<b>Aim:</b> This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle.<p> <b>Material and Method:</b> Localization of these molecules was determined by indirect...
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| Format: | Article |
| Language: | English |
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Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=556 |
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| author | Musa Karaman Hasan Özen Serpil Dağ Erginsoy Mitat Şahin |
| author_facet | Musa Karaman Hasan Özen Serpil Dağ Erginsoy Mitat Şahin |
| author_sort | Musa Karaman |
| collection | DOAJ |
| description | <b>Aim:</b> This study was performed to investigate the role of
ubiquitin and 8-hydroxyguanosine (8-OHG) expression in
liver and kidney tissue damage in Leptospira-infected cattle.<p>
<b>Material and Method:</b> Localization of these molecules was
determined by indirect immunoperoxydase method using
specific polyclonal antibodies in Leptospira positive (n=7)
and healthy control animals (n=7).<p>
<b>Results:</b> A cytoplasmic immunostaining in the hepatocytes
and kidney tubule epithelia against ubiquitin antibody was
detected in Leptospira-infected animals. Immunostaining
against 8-OHG antibody was seen intracytoplasmic and/or
intranuclear in the hepatocytes, while it was mostly intracytoplasmic
in kidney tubule epithelia.<p>
<b>Conclusion:</b> As ubiquitin and 8-OHG were shown to play
roles in pathogenesis of Leptospirosis, further detailed
studies to investigate and to wholly understand the mode of
mechanisms of these molecules are suggested. |
| format | Article |
| id | doaj-art-75176e4f09314c4a830bbca3970c8dba |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-75176e4f09314c4a830bbca3970c8dba2025-01-02T22:36:20ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532613338556Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosisMusa Karaman0Hasan Özen1Serpil Dağ Erginsoy2Mitat Şahin3Kafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD, Kars, TürkiyeKafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD, Kars, TürkiyeKafkas Üniversitesi, Veteriner Fakültesi, Patoloji AD, Kars, TürkiyeKafkas Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji AD, Paşaçayırı, Kars, Türkiye<b>Aim:</b> This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle.<p> <b>Material and Method:</b> Localization of these molecules was determined by indirect immunoperoxydase method using specific polyclonal antibodies in Leptospira positive (n=7) and healthy control animals (n=7).<p> <b>Results:</b> A cytoplasmic immunostaining in the hepatocytes and kidney tubule epithelia against ubiquitin antibody was detected in Leptospira-infected animals. Immunostaining against 8-OHG antibody was seen intracytoplasmic and/or intranuclear in the hepatocytes, while it was mostly intracytoplasmic in kidney tubule epithelia.<p> <b>Conclusion:</b> As ubiquitin and 8-OHG were shown to play roles in pathogenesis of Leptospirosis, further detailed studies to investigate and to wholly understand the mode of mechanisms of these molecules are suggested.http://eurasianjvetsci.org/pdf.php3?id=556leptospirosisubiquitin8-hydroxyguanosinepathogenesiscattle |
| spellingShingle | Musa Karaman Hasan Özen Serpil Dağ Erginsoy Mitat Şahin Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis Eurasian Journal of Veterinary Sciences leptospirosis ubiquitin 8-hydroxyguanosine pathogenesis cattle |
| title | Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis |
| title_full | Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis |
| title_fullStr | Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis |
| title_full_unstemmed | Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis |
| title_short | Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis |
| title_sort | immunohistochemical investigation of the role of ubiquitin and 8 hydroxyguanosine in pathogenesis of cattle leptospirosis |
| topic | leptospirosis ubiquitin 8-hydroxyguanosine pathogenesis cattle |
| url | http://eurasianjvetsci.org/pdf.php3?id=556 |
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