Immunohistochemical investigation of the role of ubiquitin and 8-hydroxyguanosine in pathogenesis of cattle leptospirosis
<b>Aim:</b> This study was performed to investigate the role of ubiquitin and 8-hydroxyguanosine (8-OHG) expression in liver and kidney tissue damage in Leptospira-infected cattle.<p> <b>Material and Method:</b> Localization of these molecules was determined by indirect...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=556 |
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| Summary: | <b>Aim:</b> This study was performed to investigate the role of
ubiquitin and 8-hydroxyguanosine (8-OHG) expression in
liver and kidney tissue damage in Leptospira-infected cattle.<p>
<b>Material and Method:</b> Localization of these molecules was
determined by indirect immunoperoxydase method using
specific polyclonal antibodies in Leptospira positive (n=7)
and healthy control animals (n=7).<p>
<b>Results:</b> A cytoplasmic immunostaining in the hepatocytes
and kidney tubule epithelia against ubiquitin antibody was
detected in Leptospira-infected animals. Immunostaining
against 8-OHG antibody was seen intracytoplasmic and/or
intranuclear in the hepatocytes, while it was mostly intracytoplasmic
in kidney tubule epithelia.<p>
<b>Conclusion:</b> As ubiquitin and 8-OHG were shown to play
roles in pathogenesis of Leptospirosis, further detailed
studies to investigate and to wholly understand the mode of
mechanisms of these molecules are suggested. |
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| ISSN: | 1309-6958 2146-1953 |