Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18
Abstract Background Myoblasts serve as the fundamental building blocks of muscle fibers, and there is a positive correlation between the diameter of myofibers during the juvenile phase and the rate of muscle growth, which does not change in adulthood. However, the molecular mechanisms governing myof...
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BMC
2025-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-11183-4 |
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| author | Kunyu Li Yize Song Yekai Fan Hui Zhang Mingxing Chu Yufang Liu |
| author_facet | Kunyu Li Yize Song Yekai Fan Hui Zhang Mingxing Chu Yufang Liu |
| author_sort | Kunyu Li |
| collection | DOAJ |
| description | Abstract Background Myoblasts serve as the fundamental building blocks of muscle fibers, and there is a positive correlation between the diameter of myofibers during the juvenile phase and the rate of muscle growth, which does not change in adulthood. However, the molecular mechanisms governing myofiber diameter across various developmental stages in goats remain largely unclear. Results In this study, we examined miRNA expression in the longissimus dorsi muscle tissue of goats at two distinct ages: one month, a period characterized by robust muscle growth, and nine months, when muscle development plateaus in adulthood. A total of 408 known miRNAs and 86 novel miRNAs were identified, with 32 miRNAs exhibiting differential expression between the two groups. A functional enrichment analysis of these targeted genes revealed significant enrichment in pathways closely correlated with skeletal muscle growth, development, and senescence. Notably, chi-miR-103-3p was identified among the DE miRNAs and appeared to play an important role in skeletal myoblast proliferation. Bioinformatics analysis, complemented by dual luciferase activity assays revealed that chi-miR-103-3p specifically targets the 3’UTR of FGF18. Subsequent cell transfection experiments demonstrated that chi-miR-103-3p suppresses the expression of FGF18 in goat myoblasts, thereby inhibiting cell proliferation. Moreover, FGF18 was observed to enhance the proliferation of goat myoblasts. Conclusions Collectively, our data indicated that the elevated expression of chi-miR-103-3p in adult goat myoblasts significantly repressed FGF18 expression, thereby limiting rapid muscle growth. Proliferation and differentiation of myoblasts can affect myofiber number and cell volume expansion. These findings lay the foundation for further elucidation of the molecular mechanisms underlying muscle growth and development across different life stages of goats. Additionally, it could be a potential molecular marker for improving muscle production in goats. |
| format | Article |
| id | doaj-art-74df43ae83914b5da4b4a96d3b446bfe |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-74df43ae83914b5da4b4a96d3b446bfe2025-01-12T12:09:12ZengBMCBMC Genomics1471-21642025-01-0126111510.1186/s12864-024-11183-4Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18Kunyu Li0Yize Song1Yekai Fan2Hui Zhang3Mingxing Chu4Yufang Liu5State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural SciencesAbstract Background Myoblasts serve as the fundamental building blocks of muscle fibers, and there is a positive correlation between the diameter of myofibers during the juvenile phase and the rate of muscle growth, which does not change in adulthood. However, the molecular mechanisms governing myofiber diameter across various developmental stages in goats remain largely unclear. Results In this study, we examined miRNA expression in the longissimus dorsi muscle tissue of goats at two distinct ages: one month, a period characterized by robust muscle growth, and nine months, when muscle development plateaus in adulthood. A total of 408 known miRNAs and 86 novel miRNAs were identified, with 32 miRNAs exhibiting differential expression between the two groups. A functional enrichment analysis of these targeted genes revealed significant enrichment in pathways closely correlated with skeletal muscle growth, development, and senescence. Notably, chi-miR-103-3p was identified among the DE miRNAs and appeared to play an important role in skeletal myoblast proliferation. Bioinformatics analysis, complemented by dual luciferase activity assays revealed that chi-miR-103-3p specifically targets the 3’UTR of FGF18. Subsequent cell transfection experiments demonstrated that chi-miR-103-3p suppresses the expression of FGF18 in goat myoblasts, thereby inhibiting cell proliferation. Moreover, FGF18 was observed to enhance the proliferation of goat myoblasts. Conclusions Collectively, our data indicated that the elevated expression of chi-miR-103-3p in adult goat myoblasts significantly repressed FGF18 expression, thereby limiting rapid muscle growth. Proliferation and differentiation of myoblasts can affect myofiber number and cell volume expansion. These findings lay the foundation for further elucidation of the molecular mechanisms underlying muscle growth and development across different life stages of goats. Additionally, it could be a potential molecular marker for improving muscle production in goats.https://doi.org/10.1186/s12864-024-11183-4GoatmiRNA-seqSkeletal myoblast proliferationchi-miR-103-3pFGF18 |
| spellingShingle | Kunyu Li Yize Song Yekai Fan Hui Zhang Mingxing Chu Yufang Liu Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 BMC Genomics Goat miRNA-seq Skeletal myoblast proliferation chi-miR-103-3p FGF18 |
| title | Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 |
| title_full | Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 |
| title_fullStr | Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 |
| title_full_unstemmed | Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 |
| title_short | Transcriptome integration analysis revealed that miR-103-3p regulates goat myoblast proliferation by targeting FGF18 |
| title_sort | transcriptome integration analysis revealed that mir 103 3p regulates goat myoblast proliferation by targeting fgf18 |
| topic | Goat miRNA-seq Skeletal myoblast proliferation chi-miR-103-3p FGF18 |
| url | https://doi.org/10.1186/s12864-024-11183-4 |
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