One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line
Colon cancer presents significant challenges in treatment efficiency and patient outcomes, necessitating innovative, effective strategies due to the adverse effects of conventional therapies. The study aimed to investigate the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous extra...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2024-12-01
|
| Series: | Main Group Metal Chemistry |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/mgmc-2024-0016 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846129249109409792 |
|---|---|
| author | Paranthaman Sathishbabu Shivakumar C. S. Kalaipriya S. Venkatesh Hosur Narayanappa Gireesha J. Pasha Shuaib Shazly Gamal A. Anandan Satish Shivamallu Chandan Kollur Shiva Prasad |
| author_facet | Paranthaman Sathishbabu Shivakumar C. S. Kalaipriya S. Venkatesh Hosur Narayanappa Gireesha J. Pasha Shuaib Shazly Gamal A. Anandan Satish Shivamallu Chandan Kollur Shiva Prasad |
| author_sort | Paranthaman Sathishbabu |
| collection | DOAJ |
| description | Colon cancer presents significant challenges in treatment efficiency and patient outcomes, necessitating innovative, effective strategies due to the adverse effects of conventional therapies. The study aimed to investigate the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous extract of Morus laevigata (ML) leaves and their potential biomedical applications. ML-ZnO NPs were characterized using analytical techniques. The absorption band at 330 nm was detected by UV–Vis spectroscopy, confirming the formation of ML-ZnO NPs. An average size of 68.5 ± 1.85 nm and a negative zeta potential of −11.1 ± 0.98 mV confirm the stability of nanoparticles in colloidal solutions. FTIR analysis confirmed ML-ZnO NPs, with absorption bands corresponding to Zn–O stretching vibrations and aliphatic CH and CH2 groups. SEM examination revealed diverse patterns including cauliflower-like formations. Further anticancer activity of ML-ZnO NPs was assessed through cell viability, cell cycle, and apoptosis assays. ML-ZnO NPs inhibited HT-29 cell growth dose dependently, exceeding cisplatin 56.33 ± 0.87% cell viability. Cell cycle arrest at the G2M phase in HT-29 cells treated with ML-ZnO NPs was noticeable, further validated by apoptosis, which showed large increases in apoptotic cell populations. These results may pave the way for future research investigating the potency of ZnO NPs derived from plant extracts to manage colon cancer. |
| format | Article |
| id | doaj-art-7420925b8d244af293e47483e08cf3cf |
| institution | Kabale University |
| issn | 2191-0219 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Main Group Metal Chemistry |
| spelling | doaj-art-7420925b8d244af293e47483e08cf3cf2024-12-10T07:31:43ZengDe GruyterMain Group Metal Chemistry2191-02192024-12-014711546595710.1515/mgmc-2024-0016One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell lineParanthaman Sathishbabu0Shivakumar C. S.1Kalaipriya S.2Venkatesh Hosur Narayanappa3Gireesha J.4Pasha Shuaib5Shazly Gamal A.6Anandan Satish7Shivamallu Chandan8Kollur Shiva Prasad9Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Tamaka, 563 103, Karnataka, IndiaDepartment of Clinical Nutrition and Dietetics, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Tamaka, 563 103, Karnataka, IndiaDepartment of Clinical Nutrition and Dietetics, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Tamaka, 563 103, Karnataka, IndiaResearch & Development, Miklens Bio Private Limited, Bengaluru, 560 049, Karnataka, IndiaDepartment of Studies in Environmental Science, Karnataka State Open University, Mukthagangotri, Mysuru, 570 006, Karnataka, IndiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysuru, Karnataka, 570 015, IndiaDepartment of Pharmaceutics, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh, 11451, Saudi ArabiaDepartment of Cell Biology and Molecular Genetics, Sri Devaraj Urs Medical College, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, Tamaka, 563 103, Karnataka, IndiaDepartment of Biotechnology and Bioinformatics, JSS Academy of Higher Education & Research, Mysuru, Karnataka, 570 015, IndiaSchool of Physical Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru, 570 026, Karnataka, IndiaColon cancer presents significant challenges in treatment efficiency and patient outcomes, necessitating innovative, effective strategies due to the adverse effects of conventional therapies. The study aimed to investigate the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous extract of Morus laevigata (ML) leaves and their potential biomedical applications. ML-ZnO NPs were characterized using analytical techniques. The absorption band at 330 nm was detected by UV–Vis spectroscopy, confirming the formation of ML-ZnO NPs. An average size of 68.5 ± 1.85 nm and a negative zeta potential of −11.1 ± 0.98 mV confirm the stability of nanoparticles in colloidal solutions. FTIR analysis confirmed ML-ZnO NPs, with absorption bands corresponding to Zn–O stretching vibrations and aliphatic CH and CH2 groups. SEM examination revealed diverse patterns including cauliflower-like formations. Further anticancer activity of ML-ZnO NPs was assessed through cell viability, cell cycle, and apoptosis assays. ML-ZnO NPs inhibited HT-29 cell growth dose dependently, exceeding cisplatin 56.33 ± 0.87% cell viability. Cell cycle arrest at the G2M phase in HT-29 cells treated with ML-ZnO NPs was noticeable, further validated by apoptosis, which showed large increases in apoptotic cell populations. These results may pave the way for future research investigating the potency of ZnO NPs derived from plant extracts to manage colon cancer.https://doi.org/10.1515/mgmc-2024-0016green synthesiszinc oxide nanoparticlesmorus laevigatacolon cancer |
| spellingShingle | Paranthaman Sathishbabu Shivakumar C. S. Kalaipriya S. Venkatesh Hosur Narayanappa Gireesha J. Pasha Shuaib Shazly Gamal A. Anandan Satish Shivamallu Chandan Kollur Shiva Prasad One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line Main Group Metal Chemistry green synthesis zinc oxide nanoparticles morus laevigata colon cancer |
| title | One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line |
| title_full | One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line |
| title_fullStr | One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line |
| title_full_unstemmed | One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line |
| title_short | One-pot green synthesis of zinc oxide nanoparticles using Morus laevigata aqueous extract and evaluation of its anticancer potential against HT-29 cell line |
| title_sort | one pot green synthesis of zinc oxide nanoparticles using morus laevigata aqueous extract and evaluation of its anticancer potential against ht 29 cell line |
| topic | green synthesis zinc oxide nanoparticles morus laevigata colon cancer |
| url | https://doi.org/10.1515/mgmc-2024-0016 |
| work_keys_str_mv | AT paranthamansathishbabu onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT shivakumarcs onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT kalaipriyas onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT venkateshhosurnarayanappa onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT gireeshaj onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT pashashuaib onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT shazlygamala onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT anandansatish onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT shivamalluchandan onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline AT kollurshivaprasad onepotgreensynthesisofzincoxidenanoparticlesusingmoruslaevigataaqueousextractandevaluationofitsanticancerpotentialagainstht29cellline |