Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris)
Lentil (Lens culinaris) is a very important cold-season nutritious legume crop. The crop faces intermittent drought in South Asian countries and terminal drought in West Asian and North African Mediterranean regions causing adverse impact on lentil productivity. The present study aimed to evaluate a...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Current Plant Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214662825000994 |
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| author | Neteti Siddartha Kumar Renu Pandey Anjali Anand Amit Kumar Singh Muraleedhar S. Aski Gyan Prakash Mishra Harsh Kumar Dikshit Mahesh Rao R.S. Bana Shiv Kumar Viswanathan Chinnusamy Ruchi Bansal |
| author_facet | Neteti Siddartha Kumar Renu Pandey Anjali Anand Amit Kumar Singh Muraleedhar S. Aski Gyan Prakash Mishra Harsh Kumar Dikshit Mahesh Rao R.S. Bana Shiv Kumar Viswanathan Chinnusamy Ruchi Bansal |
| author_sort | Neteti Siddartha Kumar |
| collection | DOAJ |
| description | Lentil (Lens culinaris) is a very important cold-season nutritious legume crop. The crop faces intermittent drought in South Asian countries and terminal drought in West Asian and North African Mediterranean regions causing adverse impact on lentil productivity. The present study aimed to evaluate a diverse lentil panel (243 genotypes) under irrigated and drought conditions at seedling stage and to identify significant marker trait associations for drought tolerance traits. Drought stress was imposed by restricting the pre-sowing irrigation. A total of 18 different morpho-physiological traits including root (length, surface area, volume, tips and forks), physiological (germination percentage, NDVI, canopy temperature) and growth (seedling vigor, plant biomass) traits were recorded among the lentil genotypes in both control and stress conditions. All the traits except canopy temperature were found to be significantly reduced under stress. Principal component analysis explained 56.3 % variation in control and 60.7 % variation in drought condition. Shoot dry weight had significant correlation to NDVI, shoot branching, primary and total root length, and root length density. Genotypes IC560032, IC560246, P3227, IC560051, and IG134349 were identified as drought-tolerant using SSI (<0.5). Association mapping analysis identified 65 and 71 non-overlapping distinct SNPs significantly associated with all traits under control and drought conditions, respectively. Putative candidate genes encoding legumain-like cysteine endopeptidase, L-ascorbate oxidase, and auxin-responsive proteins were involved in the regulation of key drought tolerance associated traits like germination percentage, root length, seedling vigor respectively. These findings highlight the potential of lentil germplasm for drought resilience and provide a valuable genetic resource for breeding high-yielding, stress-tolerant varieties. |
| format | Article |
| id | doaj-art-73e0bab0f68c4dbcb6355a4c27e77b42 |
| institution | Kabale University |
| issn | 2214-6628 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Plant Biology |
| spelling | doaj-art-73e0bab0f68c4dbcb6355a4c27e77b422025-08-20T05:06:57ZengElsevierCurrent Plant Biology2214-66282025-09-014310053110.1016/j.cpb.2025.100531Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris)Neteti Siddartha Kumar0Renu Pandey1Anjali Anand2Amit Kumar Singh3Muraleedhar S. Aski4Gyan Prakash Mishra5Harsh Kumar Dikshit6Mahesh Rao7R.S. Bana8Shiv Kumar9Viswanathan Chinnusamy10Ruchi Bansal11Division of Plant Physiology, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, IndiaDivision of Plant Physiology, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, IndiaDivision of Plant Physiology, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, IndiaICAR-National Bureau of Plant Genetic Resources, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, IndiaDivision of Genetics, ICAR, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR, Indian Agricultural Research Institute, New Delhi 110012, IndiaDivision of Genetics, ICAR, Indian Agricultural Research Institute, New Delhi 110012, IndiaICAR, National Institute of plant Biotechnology, New Delhi 110012, IndiaDivision of Agronomy, ICAR, Indian Agricultural Research Institute, New Delhi 110012, IndiaInternational Center for Agricultural Research in the Dry Areas, New Delhi 110012, IndiaDivision of Plant Physiology, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, IndiaDivision of Plant Physiology, ICAR, Indian Agricultural Research Institute (IARI), New Delhi 110012, India; Corresponding author.Lentil (Lens culinaris) is a very important cold-season nutritious legume crop. The crop faces intermittent drought in South Asian countries and terminal drought in West Asian and North African Mediterranean regions causing adverse impact on lentil productivity. The present study aimed to evaluate a diverse lentil panel (243 genotypes) under irrigated and drought conditions at seedling stage and to identify significant marker trait associations for drought tolerance traits. Drought stress was imposed by restricting the pre-sowing irrigation. A total of 18 different morpho-physiological traits including root (length, surface area, volume, tips and forks), physiological (germination percentage, NDVI, canopy temperature) and growth (seedling vigor, plant biomass) traits were recorded among the lentil genotypes in both control and stress conditions. All the traits except canopy temperature were found to be significantly reduced under stress. Principal component analysis explained 56.3 % variation in control and 60.7 % variation in drought condition. Shoot dry weight had significant correlation to NDVI, shoot branching, primary and total root length, and root length density. Genotypes IC560032, IC560246, P3227, IC560051, and IG134349 were identified as drought-tolerant using SSI (<0.5). Association mapping analysis identified 65 and 71 non-overlapping distinct SNPs significantly associated with all traits under control and drought conditions, respectively. Putative candidate genes encoding legumain-like cysteine endopeptidase, L-ascorbate oxidase, and auxin-responsive proteins were involved in the regulation of key drought tolerance associated traits like germination percentage, root length, seedling vigor respectively. These findings highlight the potential of lentil germplasm for drought resilience and provide a valuable genetic resource for breeding high-yielding, stress-tolerant varieties.http://www.sciencedirect.com/science/article/pii/S2214662825000994DroughtLentilQTLMarkerPhenotypingRoot |
| spellingShingle | Neteti Siddartha Kumar Renu Pandey Anjali Anand Amit Kumar Singh Muraleedhar S. Aski Gyan Prakash Mishra Harsh Kumar Dikshit Mahesh Rao R.S. Bana Shiv Kumar Viswanathan Chinnusamy Ruchi Bansal Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) Current Plant Biology Drought Lentil QTL Marker Phenotyping Root |
| title | Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) |
| title_full | Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) |
| title_fullStr | Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) |
| title_full_unstemmed | Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) |
| title_short | Genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil (Lens culinaris) |
| title_sort | genome wide association mapping reveals genetic loci and candidate genes for seedling stage drought tolerance in lentil lens culinaris |
| topic | Drought Lentil QTL Marker Phenotyping Root |
| url | http://www.sciencedirect.com/science/article/pii/S2214662825000994 |
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