Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings
Abstract The recently identified novel duck reovirus (NDRV) is a waterfowl reovirus that can seriously harm or kill various waterfowl species. However, how NDRV interacts with host cells in Muscovy ducklings beyond the typical pathogenesis resulting from a viral infection is unknown. The current stu...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-07259-2 |
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| author | Tao Yun Jionggang Hua Liu Chen Weicheng Ye Zheng Ni Yinchu Zhu Cun Zhang |
| author_facet | Tao Yun Jionggang Hua Liu Chen Weicheng Ye Zheng Ni Yinchu Zhu Cun Zhang |
| author_sort | Tao Yun |
| collection | DOAJ |
| description | Abstract The recently identified novel duck reovirus (NDRV) is a waterfowl reovirus that can seriously harm or kill various waterfowl species. However, how NDRV interacts with host cells in Muscovy ducklings beyond the typical pathogenesis resulting from a viral infection is unknown. The current study examined the global translation efficiency of the Fabricius bursa of Muscovy ducklings infected with NDRV HN10 using mass spectrometry and ribosome footprint sequencing. Protein-protein interactions were investigated using immunogold labeling, transmission electron microscopy, and immunocytochemistry. An analysis of the relationship between m6A and translation was performed using RNA immunoprecipitation and m6A methylation immunoprecipitation. We found that both in vivo and in vitro, the translation efficiency of RNA modified with m6A could be significantly reduced by σB, a structural protein component of NDRV HN10. Furthermore, σB might simultaneously interact with the stress granule complex CAPRIN1 and G3BP1 and the m6A reader protein YTHDF1/3. Significant overlap was observed between m6A-modified and G3BP1-enriched RNA, indicating that granule stress could capture m6A-methylated RNA. We discovered a new function for NDRV HN10 in translational shutoff by recruiting m6A-modified RNA into stress granules located in the Fabricius bursa of Muscovy ducklings. |
| format | Article |
| id | doaj-art-73c5bc7b3fb044f0aed4fb238d9ddb33 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-73c5bc7b3fb044f0aed4fb238d9ddb332024-11-24T12:39:04ZengNature PortfolioCommunications Biology2399-36422024-11-017111010.1038/s42003-024-07259-2Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklingsTao Yun0Jionggang Hua1Liu Chen2Weicheng Ye3Zheng Ni4Yinchu Zhu5Cun Zhang6State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural SciencesAbstract The recently identified novel duck reovirus (NDRV) is a waterfowl reovirus that can seriously harm or kill various waterfowl species. However, how NDRV interacts with host cells in Muscovy ducklings beyond the typical pathogenesis resulting from a viral infection is unknown. The current study examined the global translation efficiency of the Fabricius bursa of Muscovy ducklings infected with NDRV HN10 using mass spectrometry and ribosome footprint sequencing. Protein-protein interactions were investigated using immunogold labeling, transmission electron microscopy, and immunocytochemistry. An analysis of the relationship between m6A and translation was performed using RNA immunoprecipitation and m6A methylation immunoprecipitation. We found that both in vivo and in vitro, the translation efficiency of RNA modified with m6A could be significantly reduced by σB, a structural protein component of NDRV HN10. Furthermore, σB might simultaneously interact with the stress granule complex CAPRIN1 and G3BP1 and the m6A reader protein YTHDF1/3. Significant overlap was observed between m6A-modified and G3BP1-enriched RNA, indicating that granule stress could capture m6A-methylated RNA. We discovered a new function for NDRV HN10 in translational shutoff by recruiting m6A-modified RNA into stress granules located in the Fabricius bursa of Muscovy ducklings.https://doi.org/10.1038/s42003-024-07259-2 |
| spellingShingle | Tao Yun Jionggang Hua Liu Chen Weicheng Ye Zheng Ni Yinchu Zhu Cun Zhang Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings Communications Biology |
| title | Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings |
| title_full | Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings |
| title_fullStr | Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings |
| title_full_unstemmed | Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings |
| title_short | Infection with novel duck reovirus induces stress granule and methylation-mediated host translational shutoff in Muscovy ducklings |
| title_sort | infection with novel duck reovirus induces stress granule and methylation mediated host translational shutoff in muscovy ducklings |
| url | https://doi.org/10.1038/s42003-024-07259-2 |
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