Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors
This work investigated the preparation of specific targeted drug delivery systems in cancer chemotherapy by folate conjugated human serum albumin nanoparticles encapsulated resveratrol (RES) nanoparticles (FA-HSA-RESNPs). FA was coupled to HSA, and RES was encapsulated in FA-conjugated HSA by high p...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2018.1548468 |
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| author | Bolin Lian Mingfang Wu Ziqi Feng Yiping Deng Chen Zhong Xiuhua Zhao |
| author_facet | Bolin Lian Mingfang Wu Ziqi Feng Yiping Deng Chen Zhong Xiuhua Zhao |
| author_sort | Bolin Lian |
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| description | This work investigated the preparation of specific targeted drug delivery systems in cancer chemotherapy by folate conjugated human serum albumin nanoparticles encapsulated resveratrol (RES) nanoparticles (FA-HSA-RESNPs). FA was coupled to HSA, and RES was encapsulated in FA-conjugated HSA by high pressure fluid nano-homogeneous emulsification. The average particle size and polydispersity index of NPs prepared under optimal conditions were 102.1 ± 4.9 nm and 0.001. The drug capsulation efficiency and drug loading efficiency were 98.36 and 14.66%, respectively. The analysis of the results of the physical characterization showed that the RES was present in the FA-HSA-RESNPs in an amorphous state. In vitro drug-release study showed that the NPs can release the drug persistently and slowly. The inhibition rate of FA-HSA-RESNPs and RES was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide method to be 110.8 and 157.2 μM, respectively. The targeting ability of the FA-HSA-RESNPs for the HepG2 cell was measured by fluorescein isothiocyanate-modified albumin techniques. The uptake rate of FA-HSA-RESNPs was higher than that of the original RES. By using near-infrared imaging, in vivo activity was labeled with Cy5 fluorescent FA-HSA-RESNP confirmed FA-HSA-RESNP tumor-targeting ability. The intravenous administration bioavailability of FA-HSA-RESNPs was about 5.95-fold higher than that of the original RES. |
| format | Article |
| id | doaj-art-73a903e6fa324e1b93657acf5d6da5db |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-73a903e6fa324e1b93657acf5d6da5db2025-08-20T03:51:19ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-0147115416510.1080/21691401.2018.1548468Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumorsBolin Lian0Mingfang Wu1Ziqi Feng2Yiping Deng3Chen Zhong4Xiuhua Zhao5Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, ChinaCollege of Life Science, Northeast Forestry University, Harbin, ChinaKey Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin, ChinaThis work investigated the preparation of specific targeted drug delivery systems in cancer chemotherapy by folate conjugated human serum albumin nanoparticles encapsulated resveratrol (RES) nanoparticles (FA-HSA-RESNPs). FA was coupled to HSA, and RES was encapsulated in FA-conjugated HSA by high pressure fluid nano-homogeneous emulsification. The average particle size and polydispersity index of NPs prepared under optimal conditions were 102.1 ± 4.9 nm and 0.001. The drug capsulation efficiency and drug loading efficiency were 98.36 and 14.66%, respectively. The analysis of the results of the physical characterization showed that the RES was present in the FA-HSA-RESNPs in an amorphous state. In vitro drug-release study showed that the NPs can release the drug persistently and slowly. The inhibition rate of FA-HSA-RESNPs and RES was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide method to be 110.8 and 157.2 μM, respectively. The targeting ability of the FA-HSA-RESNPs for the HepG2 cell was measured by fluorescein isothiocyanate-modified albumin techniques. The uptake rate of FA-HSA-RESNPs was higher than that of the original RES. By using near-infrared imaging, in vivo activity was labeled with Cy5 fluorescent FA-HSA-RESNP confirmed FA-HSA-RESNP tumor-targeting ability. The intravenous administration bioavailability of FA-HSA-RESNPs was about 5.95-fold higher than that of the original RES.https://www.tandfonline.com/doi/10.1080/21691401.2018.1548468Resveratrolfolatenanoparticlesbioavailabilitynoninvasive live animal imaging technologytargeted drug delivery |
| spellingShingle | Bolin Lian Mingfang Wu Ziqi Feng Yiping Deng Chen Zhong Xiuhua Zhao Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors Artificial Cells, Nanomedicine, and Biotechnology Resveratrol folate nanoparticles bioavailability noninvasive live animal imaging technology targeted drug delivery |
| title | Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors |
| title_full | Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors |
| title_fullStr | Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors |
| title_full_unstemmed | Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors |
| title_short | Folate-conjugated human serum albumin-encapsulated resveratrol nanoparticles: preparation, characterization, bioavailability and targeting of liver tumors |
| title_sort | folate conjugated human serum albumin encapsulated resveratrol nanoparticles preparation characterization bioavailability and targeting of liver tumors |
| topic | Resveratrol folate nanoparticles bioavailability noninvasive live animal imaging technology targeted drug delivery |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2018.1548468 |
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