Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes
Stroke induces a strong inflammatory reaction in the brain and depresses the immune system. We sought to assess longitudinal changes in T-cell numbers in the lymphoid organs of living mice after brain ischemia. Middle cerebral artery occlusion was carried out in transgenic mice expressing green fluo...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2008-07-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2008.00016 |
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| author | Abraham Martin Juan Aguirre Ana Sarasa-Renedo Debbie Tsoukatou Anikitos Garofalakis Heiko Meyer Clio Mamalaki Jorge Ripoll Anna M. Planas |
| author_facet | Abraham Martin Juan Aguirre Ana Sarasa-Renedo Debbie Tsoukatou Anikitos Garofalakis Heiko Meyer Clio Mamalaki Jorge Ripoll Anna M. Planas |
| author_sort | Abraham Martin |
| collection | DOAJ |
| description | Stroke induces a strong inflammatory reaction in the brain and depresses the immune system. We sought to assess longitudinal changes in T-cell numbers in the lymphoid organs of living mice after brain ischemia. Middle cerebral artery occlusion was carried out in transgenic mice expressing green fluorescent protein (GFP + ) in the T-cell population under the control of the hCD2 locus control region. Imaging was performed by three-dimensional fluorescence molecular tomography (FMT) before and at several time points after ischemia or sham operation and in controls. At day 7, GFP + cell content in lymphoid organs was measured postmortem by flow cytometry. GFP + cell numbers and in vivo FMT signal intensity were reduced at day 7 after ischemia and, to a lesser extent, after sham operation. Linear regression analysis demonstrated that postmortem GFP + cell numbers and corresponding in vivo FMT data were significantly correlated in the thymus ( r 2 = .65, p < .0001) and lymph nodes ( r 2 = .67, p < .0001). These relationships allowed inferring the number of GFP + T cells from in vivo FMT data. The results show the time course reduction of T-cell content in the lymphoid organs of living mice, providing in vivo evidence of lymphoid organ atrophy after stroke and, to a lesser extent, after head surgery with craniectomy and dura mater opening in sham-operated mice. |
| format | Article |
| id | doaj-art-72abfcf11b7a434eb218b798b9565f70 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2008-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-72abfcf11b7a434eb218b798b9565f702025-01-02T02:58:23ZengSAGE PublishingMolecular Imaging1536-01212008-07-01710.2310/7290.2008.0001610.2310_7290.2008.00016Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T LymphocytesAbraham MartinJuan AguirreAna Sarasa-RenedoDebbie TsoukatouAnikitos GarofalakisHeiko MeyerClio MamalakiJorge RipollAnna M. PlanasStroke induces a strong inflammatory reaction in the brain and depresses the immune system. We sought to assess longitudinal changes in T-cell numbers in the lymphoid organs of living mice after brain ischemia. Middle cerebral artery occlusion was carried out in transgenic mice expressing green fluorescent protein (GFP + ) in the T-cell population under the control of the hCD2 locus control region. Imaging was performed by three-dimensional fluorescence molecular tomography (FMT) before and at several time points after ischemia or sham operation and in controls. At day 7, GFP + cell content in lymphoid organs was measured postmortem by flow cytometry. GFP + cell numbers and in vivo FMT signal intensity were reduced at day 7 after ischemia and, to a lesser extent, after sham operation. Linear regression analysis demonstrated that postmortem GFP + cell numbers and corresponding in vivo FMT data were significantly correlated in the thymus ( r 2 = .65, p < .0001) and lymph nodes ( r 2 = .67, p < .0001). These relationships allowed inferring the number of GFP + T cells from in vivo FMT data. The results show the time course reduction of T-cell content in the lymphoid organs of living mice, providing in vivo evidence of lymphoid organ atrophy after stroke and, to a lesser extent, after head surgery with craniectomy and dura mater opening in sham-operated mice.https://doi.org/10.2310/7290.2008.00016 |
| spellingShingle | Abraham Martin Juan Aguirre Ana Sarasa-Renedo Debbie Tsoukatou Anikitos Garofalakis Heiko Meyer Clio Mamalaki Jorge Ripoll Anna M. Planas Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes Molecular Imaging |
| title | Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes |
| title_full | Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes |
| title_fullStr | Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes |
| title_full_unstemmed | Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes |
| title_short | Imaging Changes in Lymphoid Organs In Vivo after Brain Ischemia with Three-Dimensional Fluorescence Molecular Tomography in Transgenic Mice Expressing Green Fluorescent Protein in T Lymphocytes |
| title_sort | imaging changes in lymphoid organs in vivo after brain ischemia with three dimensional fluorescence molecular tomography in transgenic mice expressing green fluorescent protein in t lymphocytes |
| url | https://doi.org/10.2310/7290.2008.00016 |
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