Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development

Environmental contamination from mixtures of chemical substances and materials resulting from anthropogenic activities has adverse effects on aquatic biota. Fipronil (FP) is an insecticide widely used in agriculture and public health and is frequently detected in environmental compartments. Micropla...

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Main Authors: Flavio Kiyoshi Tominaga, Rafaella Silva Brito, Isis Wanessa Ferreira dos Santos, Amanda de Azevedo Valle, Gisele Giannocco, Rui Monteiro de Barros Maciel, Fábio Kummrow, Marilia Cristina Oliveira Souza, Ana Carolina Luchiari, Bruno Fiorelini Pereira
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Microplastics
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Online Access:https://www.mdpi.com/2673-8929/3/4/44
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author Flavio Kiyoshi Tominaga
Rafaella Silva Brito
Isis Wanessa Ferreira dos Santos
Amanda de Azevedo Valle
Gisele Giannocco
Rui Monteiro de Barros Maciel
Fábio Kummrow
Marilia Cristina Oliveira Souza
Ana Carolina Luchiari
Bruno Fiorelini Pereira
author_facet Flavio Kiyoshi Tominaga
Rafaella Silva Brito
Isis Wanessa Ferreira dos Santos
Amanda de Azevedo Valle
Gisele Giannocco
Rui Monteiro de Barros Maciel
Fábio Kummrow
Marilia Cristina Oliveira Souza
Ana Carolina Luchiari
Bruno Fiorelini Pereira
author_sort Flavio Kiyoshi Tominaga
collection DOAJ
description Environmental contamination from mixtures of chemical substances and materials resulting from anthropogenic activities has adverse effects on aquatic biota. Fipronil (FP) is an insecticide widely used in agriculture and public health and is frequently detected in environmental compartments. Microplastics (MPs) represent another class of contaminants that have raised concerns due to their ability to adsorb pollutants, potentially acting as a vector. This study evaluated the influence of polyethylene microplastics on the toxicity of FP in zebrafish (<i>Danio rerio</i>) embryos/larvae. Zebrafish embryos were exposed to aqueous suspension containing purified FP and the FP + MP mixtures for 120 h. Mortality, teratogenicity and cardiotoxicity were observed during the zebrafish development. Larvae were more sensitive to FP than the embryo. The presence of MPs reduced lethality; however, no significant changes were observed for sublethal endpoints. Our findings demonstrate the acute impact of FP on zebrafish embryos/larvae, demonstrating that MPs interfere with toxicity. Therefore, understanding the mechanisms of interaction between contaminants of different classes is essential for predicting the associated risks to biota.
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institution Kabale University
issn 2673-8929
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publishDate 2024-11-01
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series Microplastics
spelling doaj-art-72a07d281a8643a5bca82df7226e350c2024-12-27T14:41:57ZengMDPI AGMicroplastics2673-89292024-11-013471772910.3390/microplastics3040044Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic DevelopmentFlavio Kiyoshi Tominaga0Rafaella Silva Brito1Isis Wanessa Ferreira dos Santos2Amanda de Azevedo Valle3Gisele Giannocco4Rui Monteiro de Barros Maciel5Fábio Kummrow6Marilia Cristina Oliveira Souza7Ana Carolina Luchiari8Bruno Fiorelini Pereira9Laboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilDepartment of Pharmaceutical Sciences, Universidade Federal de São Paulo—Campus Diadema, São Paulo 09972-270, São Paulo, BrazilDepartment of Biomolecular Sciences, University of São Paulo, Ribeirão Preto 14040-020, São Paulo, BrazilFishLab, Department of Physiology and Behavior, Federal University of Rio Grande do Norte, Natal 59064-741, Rio Grande do Norte, BrazilLaboratory of Molecular and Translational Endocrinology, Division of Endocrinology, Department of Medicine, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039-000, São Paulo, BrazilEnvironmental contamination from mixtures of chemical substances and materials resulting from anthropogenic activities has adverse effects on aquatic biota. Fipronil (FP) is an insecticide widely used in agriculture and public health and is frequently detected in environmental compartments. Microplastics (MPs) represent another class of contaminants that have raised concerns due to their ability to adsorb pollutants, potentially acting as a vector. This study evaluated the influence of polyethylene microplastics on the toxicity of FP in zebrafish (<i>Danio rerio</i>) embryos/larvae. Zebrafish embryos were exposed to aqueous suspension containing purified FP and the FP + MP mixtures for 120 h. Mortality, teratogenicity and cardiotoxicity were observed during the zebrafish development. Larvae were more sensitive to FP than the embryo. The presence of MPs reduced lethality; however, no significant changes were observed for sublethal endpoints. Our findings demonstrate the acute impact of FP on zebrafish embryos/larvae, demonstrating that MPs interfere with toxicity. Therefore, understanding the mechanisms of interaction between contaminants of different classes is essential for predicting the associated risks to biota.https://www.mdpi.com/2673-8929/3/4/44<i>Danio rerio</i>fipronilmicroplasticmixture toxicityteratogenicity
spellingShingle Flavio Kiyoshi Tominaga
Rafaella Silva Brito
Isis Wanessa Ferreira dos Santos
Amanda de Azevedo Valle
Gisele Giannocco
Rui Monteiro de Barros Maciel
Fábio Kummrow
Marilia Cristina Oliveira Souza
Ana Carolina Luchiari
Bruno Fiorelini Pereira
Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
Microplastics
<i>Danio rerio</i>
fipronil
microplastic
mixture toxicity
teratogenicity
title Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
title_full Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
title_fullStr Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
title_full_unstemmed Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
title_short Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development
title_sort microplastic interference with fipronil toxicity to zebrafish embryonic development
topic <i>Danio rerio</i>
fipronil
microplastic
mixture toxicity
teratogenicity
url https://www.mdpi.com/2673-8929/3/4/44
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