Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.)
Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and co...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2024-11-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/55154 |
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| author | Mahat Magandhi Sobir Deden Derajat Matra Yudiwanti Wahyu Sudarmono |
| author_facet | Mahat Magandhi Sobir Deden Derajat Matra Yudiwanti Wahyu Sudarmono |
| author_sort | Mahat Magandhi |
| collection | DOAJ |
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Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources.
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| format | Article |
| id | doaj-art-726717f37a9f45d0be4b9bf6bcdfabed |
| institution | Kabale University |
| issn | 1978-3019 2086-4094 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj-art-726717f37a9f45d0be4b9bf6bcdfabed2024-11-25T03:41:15ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942024-11-0132110.4308/hjb.32.1.132-139Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.)Mahat Magandhi0Sobir1Deden Derajat Matra2Yudiwanti Wahyu3Sudarmono4Study Program of Plant Breeding and Biotechnology, Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Kampus IPB Dramaga, Bogor 16680, IndonesiaDepartment of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Kampus IPB Dramaga, Bogor 16680, IndonesiaDepartment of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Kampus IPB Dramaga, Bogor 16680, IndonesiaDepartment of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Kampus IPB Dramaga, Bogor 16680, IndonesiaResearch Center for Biosystematics and Evolution, National Research and Innovation Agency, Bogor, Indonesia Durio testudinarius, known as Durian Kura-Kura, is a native Bornean durian species in the Malvaceae family with fruit at the base of the main stem. Recent advances in genomic-based next-generation sequencing are being used to conserve germplasm and enhance plant breeding. This method rapidly and cost-effectively sequences plant genomes, allowing for simple sequence repeat markers to be constructed in silico. This study aimed to design and evaluate novel, simple sequence repeat markers utilizing next-generation sequencing microsatellite data of D. testudinarius. A total of 20 sequences containing di-, tri-, tetra-, and penta-nucleotide motif repeats were selected and designed to generate primers using Primer3 online software. The PCR results of the twenty primers tested on D. testudinarius and 19 genomic DNA samples of species from the Malvales order showed successful amplification. These newly developed simple sequence repeat markers are crucial for genetic population analyses, determining population structure, and enhancing plant breeding programs and conservation strategies for D. testudinarius and its relative's germplasm resources. https://journal.ipb.ac.id/index.php/hayati/article/view/55154 |
| spellingShingle | Mahat Magandhi Sobir Deden Derajat Matra Yudiwanti Wahyu Sudarmono Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) Hayati Journal of Biosciences |
| title | Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) |
| title_full | Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) |
| title_fullStr | Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) |
| title_full_unstemmed | Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) |
| title_short | Discovery of Simple Sequence Repeat Markers in the Endemic Durian Kura-Kura (Durio testudinarius Becc.) |
| title_sort | discovery of simple sequence repeat markers in the endemic durian kura kura durio testudinarius becc |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/55154 |
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