Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2
The rapid and accurate detection of SARS-CoV-2 in environmental settings is crucial for effective public health management during the COVID-19 pandemic. This study compares the performance of the Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) and the Reverse Transcription loo...
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MDPI AG
2024-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/13/11/1022 |
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| author | Simona Spiteri Federica Marino Luna Girolamini Maria Rosaria Pascale Carlo Derelitto Laura Caligaris Simone Paghera Sandra Cristino |
| author_facet | Simona Spiteri Federica Marino Luna Girolamini Maria Rosaria Pascale Carlo Derelitto Laura Caligaris Simone Paghera Sandra Cristino |
| author_sort | Simona Spiteri |
| collection | DOAJ |
| description | The rapid and accurate detection of SARS-CoV-2 in environmental settings is crucial for effective public health management during the COVID-19 pandemic. This study compares the performance of the Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) and the Reverse Transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection from 100 surface samples collected in healthcare environments. The reference method, RT-qPCR, identified a percentage of 25% of positive samples, while RT-LAMP detected a percentage of 27% of positive surfaces. Our findings reveal a sensitivity of 32% and specificity of 75% for RT-LAMP, with a positive predictive value of 30% and a negative predictive value of 77%. The overall accuracy and concordance with RT-qPCR was 64% for both methods. Despite its lower sensitivity compared to RT-qPCR, RT-LAMP had an advantage due to its rapid screening and environmental surveillance, which is particularly useful for confirming negative results. These results underscore the potential of RT-LAMP not only as a valuable method in the environmental monitoring of SARS-CoV-2 but also as a system to control the sanitation process in ordinary and emergency conditions, providing further optimization and validation for its reliability in routine surveillance and outbreak response efforts. |
| format | Article |
| id | doaj-art-7265d75f07a543a88c4edd36ed82dbdc |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-7265d75f07a543a88c4edd36ed82dbdc2024-11-26T18:17:02ZengMDPI AGPathogens2076-08172024-11-011311102210.3390/pathogens13111022Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2Simona Spiteri0Federica Marino1Luna Girolamini2Maria Rosaria Pascale3Carlo Derelitto4Laura Caligaris5Simone Paghera6Sandra Cristino7Department of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyCopan Italia, 25125 Brescia, ItalyDepartment of Biological, Geological, and Environmental Sciences, University of Bologna, via San Giacomo 12, 40126 Bologna, ItalyThe rapid and accurate detection of SARS-CoV-2 in environmental settings is crucial for effective public health management during the COVID-19 pandemic. This study compares the performance of the Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) and the Reverse Transcription loop-mediated isothermal amplification (RT-LAMP) for SARS-CoV-2 detection from 100 surface samples collected in healthcare environments. The reference method, RT-qPCR, identified a percentage of 25% of positive samples, while RT-LAMP detected a percentage of 27% of positive surfaces. Our findings reveal a sensitivity of 32% and specificity of 75% for RT-LAMP, with a positive predictive value of 30% and a negative predictive value of 77%. The overall accuracy and concordance with RT-qPCR was 64% for both methods. Despite its lower sensitivity compared to RT-qPCR, RT-LAMP had an advantage due to its rapid screening and environmental surveillance, which is particularly useful for confirming negative results. These results underscore the potential of RT-LAMP not only as a valuable method in the environmental monitoring of SARS-CoV-2 but also as a system to control the sanitation process in ordinary and emergency conditions, providing further optimization and validation for its reliability in routine surveillance and outbreak response efforts.https://www.mdpi.com/2076-0817/13/11/1022SARS-CoV-2COVID-19RT-qPCRRT-LAMPenvironmental surveillancepublic health |
| spellingShingle | Simona Spiteri Federica Marino Luna Girolamini Maria Rosaria Pascale Carlo Derelitto Laura Caligaris Simone Paghera Sandra Cristino Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 Pathogens SARS-CoV-2 COVID-19 RT-qPCR RT-LAMP environmental surveillance public health |
| title | Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| title_full | Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| title_fullStr | Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| title_full_unstemmed | Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| title_short | Loop-Mediated Isothermal Amplification (LAMP): An Innovative Approach for the Environmental Monitoring of SARS-CoV-2 |
| title_sort | loop mediated isothermal amplification lamp an innovative approach for the environmental monitoring of sars cov 2 |
| topic | SARS-CoV-2 COVID-19 RT-qPCR RT-LAMP environmental surveillance public health |
| url | https://www.mdpi.com/2076-0817/13/11/1022 |
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