Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought
Abstract Tartary buckwheat (Fagopyrum tataricum) is an important crop used for edible food and medicinal usage. Drought annually brings reduction in crop yield and quality, causing enormous economic losses. Transcription factors are often involved in the regulation of plant responses to environmenta...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12870-024-06019-y |
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| author | Jinbo Li Xin Yang Bianling Tian Tian Tian Yu Meng Fei Liu |
| author_facet | Jinbo Li Xin Yang Bianling Tian Tian Tian Yu Meng Fei Liu |
| author_sort | Jinbo Li |
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| description | Abstract Tartary buckwheat (Fagopyrum tataricum) is an important crop used for edible food and medicinal usage. Drought annually brings reduction in crop yield and quality, causing enormous economic losses. Transcription factors are often involved in the regulation of plant responses to environmental stresses. In this study, we identified 233 MYB transcription factors in tartary buckwheat and classified them into 13 groups, including 1R, R2R3, 3R, 4R types. Gene structure and conserved motifs of these 233 FtMYBs suggested the relative conservation of these FtMYBs within each group. There is strong collinearity within the genomes of F. tataricum, with identifying syntenic gene pairs of FtMYB. Further, the expansion of FtMYB genes was attributed to whole genome duplication. The enrichment analysis of cis-acting elements in the FtMYB genes indicated that FtMYBs may participate in abiotic stress responses. The transcriptional changes of FtMYB genes in tartary buckwheat were then investigated using public data and qPCR. A number of FtMYB genes exhibited apparent transcript levels in the detected tissues and most of them disturbed their expression after the treatment of PEG6000 or natural treatment of tartary buckwheat seedlings. Some of the FtMYB genes showed a similar expression trend with qPCR validation. FtMYB gene FtPinG0005108900.01 were shown to activated by PEG6000 and natural drought treatment, and its encoded protein localizes to nucleus, revealing it as a typical transcription factor. Overexpression of FtPinG0005108900.01 increase the drought tolerance, and transcriptome analysis indicated that lignin synthesis other than flavonoid biosynthesis pathway was activated in the overexpressing plants following drought treatment. Our results provided detailed evolution and comparative genomic information of FtMYBs in tartary buckwheat and dissected the function of a FtMYB gene FtPinG0005108900.01 in response to drought. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Plant Biology |
| spelling | doaj-art-718f337d43de46718475472e455c48712025-01-12T12:14:16ZengBMCBMC Plant Biology1471-22292025-01-0125111610.1186/s12870-024-06019-yAnalysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to droughtJinbo Li0Xin Yang1Bianling Tian2Tian Tian3Yu Meng4Fei Liu5Life Science College, Luoyang Normal UniversityState Key Laboratory of Crop Stress Adaption and Improvement, School of Life Sciences, Henan UniversityState Key Laboratory of Crop Stress Adaption and Improvement, School of Life Sciences, Henan UniversityState Key Laboratory of Crop Stress Adaption and Improvement, School of Life Sciences, Henan UniversityCollege of Landscape and Travel, Hebei Agricultural UniversityState Key Laboratory of Crop Stress Adaption and Improvement, School of Life Sciences, Henan UniversityAbstract Tartary buckwheat (Fagopyrum tataricum) is an important crop used for edible food and medicinal usage. Drought annually brings reduction in crop yield and quality, causing enormous economic losses. Transcription factors are often involved in the regulation of plant responses to environmental stresses. In this study, we identified 233 MYB transcription factors in tartary buckwheat and classified them into 13 groups, including 1R, R2R3, 3R, 4R types. Gene structure and conserved motifs of these 233 FtMYBs suggested the relative conservation of these FtMYBs within each group. There is strong collinearity within the genomes of F. tataricum, with identifying syntenic gene pairs of FtMYB. Further, the expansion of FtMYB genes was attributed to whole genome duplication. The enrichment analysis of cis-acting elements in the FtMYB genes indicated that FtMYBs may participate in abiotic stress responses. The transcriptional changes of FtMYB genes in tartary buckwheat were then investigated using public data and qPCR. A number of FtMYB genes exhibited apparent transcript levels in the detected tissues and most of them disturbed their expression after the treatment of PEG6000 or natural treatment of tartary buckwheat seedlings. Some of the FtMYB genes showed a similar expression trend with qPCR validation. FtMYB gene FtPinG0005108900.01 were shown to activated by PEG6000 and natural drought treatment, and its encoded protein localizes to nucleus, revealing it as a typical transcription factor. Overexpression of FtPinG0005108900.01 increase the drought tolerance, and transcriptome analysis indicated that lignin synthesis other than flavonoid biosynthesis pathway was activated in the overexpressing plants following drought treatment. Our results provided detailed evolution and comparative genomic information of FtMYBs in tartary buckwheat and dissected the function of a FtMYB gene FtPinG0005108900.01 in response to drought.https://doi.org/10.1186/s12870-024-06019-yFagopyrum tataricumDroughtTranscription factorLigninTranscriptome |
| spellingShingle | Jinbo Li Xin Yang Bianling Tian Tian Tian Yu Meng Fei Liu Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought BMC Plant Biology Fagopyrum tataricum Drought Transcription factor Lignin Transcriptome |
| title | Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought |
| title_full | Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought |
| title_fullStr | Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought |
| title_full_unstemmed | Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought |
| title_short | Analysis of the MYB gene family in tartary buckwheat and functional investigation of FtPinG0005108900.01 in response to drought |
| title_sort | analysis of the myb gene family in tartary buckwheat and functional investigation of ftping0005108900 01 in response to drought |
| topic | Fagopyrum tataricum Drought Transcription factor Lignin Transcriptome |
| url | https://doi.org/10.1186/s12870-024-06019-y |
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