Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia
Abstract The study aimed to explore the impact of dietary Pediastrum boryanum (PB) on growth performance, immune-physiological and transcriptome responses of Nile tilapia (Oreochromis niloticus). A 90-day trial using 1800 fish (17.00 ± 1.722 g) was allocated at random into 4 groups, three replicates...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-07498-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849238552260378624 |
|---|---|
| author | Raghad E. Soliman Ahmed Ateya Mohamed Abou-El-Atta Wasseem Emam Mohamed M. Abdel-Rahim Ashraf I. G. Elhetawy Ahmed E. Elshafey Radi A. Mohamed |
| author_facet | Raghad E. Soliman Ahmed Ateya Mohamed Abou-El-Atta Wasseem Emam Mohamed M. Abdel-Rahim Ashraf I. G. Elhetawy Ahmed E. Elshafey Radi A. Mohamed |
| author_sort | Raghad E. Soliman |
| collection | DOAJ |
| description | Abstract The study aimed to explore the impact of dietary Pediastrum boryanum (PB) on growth performance, immune-physiological and transcriptome responses of Nile tilapia (Oreochromis niloticus). A 90-day trial using 1800 fish (17.00 ± 1.722 g) was allocated at random into 4 groups, three replicates each. The 4 groups received PB at 0 (G1), 1 (G2), 2 (G3), and 4 (G4) g/kg diet, respectively. The fish in Group G3 displayed a significant (p < 0.05) increase in final body weight, weight gain, specific growth rate, and final length when compared to the control group. In addition, red blood cell counts (RBCs), and neutrophils were significantly (p < 0.05) higher in G3. The groups’ biochemical parameters did not significantly differ from one another; nevertheless, significant (p < 0.05) variations in immunoglobulin M (IgM), phagocytic activity, and the values of the phagocytic index. The antioxidative activity of superoxide dismutase (SOD) and catalase differed significantly (p < 0.05) between groups, with the highest values seen in group G3. Moreover, there was a significant (p < 0.05) upgrade in the expression levels of GH, IGF-1, SOD, catalase, IL-6, IL-8, and PGAM2 genes in group G3. Additionally, G3 had the most typical liver structure, significantly (p < 0.05) increased villi length and width, and a thicker lamina propria in the intestine. In conclusion, dietary supplementation of PB boosted growth performance, blood and serum profile, morphometric evaluation of the liver and intestine, and expression of genes related to growth, antioxidant capacity, immunity, and energy metabolism in Nile tilapia. |
| format | Article |
| id | doaj-art-70eac8d7418b439c97de89e4b4c08479 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-70eac8d7418b439c97de89e4b4c084792025-08-20T04:01:35ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-07498-1Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapiaRaghad E. Soliman0Ahmed Ateya1Mohamed Abou-El-Atta2Wasseem Emam3Mohamed M. Abdel-Rahim4Ashraf I. G. Elhetawy5Ahmed E. Elshafey6Radi A. Mohamed7Department of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh UniversityDepartment of Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura UniversityDepartment of Fish Health and Management, Central Laboratory for Aquaculture ResearchEthical Seafood ResearchAquaculture Division, National Institute of Oceanography and Fisheries, NIOFAquaculture Division, National Institute of Oceanography and Fisheries, NIOFDepartment of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh UniversityDepartment of Aquaculture, Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh UniversityAbstract The study aimed to explore the impact of dietary Pediastrum boryanum (PB) on growth performance, immune-physiological and transcriptome responses of Nile tilapia (Oreochromis niloticus). A 90-day trial using 1800 fish (17.00 ± 1.722 g) was allocated at random into 4 groups, three replicates each. The 4 groups received PB at 0 (G1), 1 (G2), 2 (G3), and 4 (G4) g/kg diet, respectively. The fish in Group G3 displayed a significant (p < 0.05) increase in final body weight, weight gain, specific growth rate, and final length when compared to the control group. In addition, red blood cell counts (RBCs), and neutrophils were significantly (p < 0.05) higher in G3. The groups’ biochemical parameters did not significantly differ from one another; nevertheless, significant (p < 0.05) variations in immunoglobulin M (IgM), phagocytic activity, and the values of the phagocytic index. The antioxidative activity of superoxide dismutase (SOD) and catalase differed significantly (p < 0.05) between groups, with the highest values seen in group G3. Moreover, there was a significant (p < 0.05) upgrade in the expression levels of GH, IGF-1, SOD, catalase, IL-6, IL-8, and PGAM2 genes in group G3. Additionally, G3 had the most typical liver structure, significantly (p < 0.05) increased villi length and width, and a thicker lamina propria in the intestine. In conclusion, dietary supplementation of PB boosted growth performance, blood and serum profile, morphometric evaluation of the liver and intestine, and expression of genes related to growth, antioxidant capacity, immunity, and energy metabolism in Nile tilapia.https://doi.org/10.1038/s41598-025-07498-1MicroalgaePediastrum boryanumNile tilapiaImmunityTranscriptomic response |
| spellingShingle | Raghad E. Soliman Ahmed Ateya Mohamed Abou-El-Atta Wasseem Emam Mohamed M. Abdel-Rahim Ashraf I. G. Elhetawy Ahmed E. Elshafey Radi A. Mohamed Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia Scientific Reports Microalgae Pediastrum boryanum Nile tilapia Immunity Transcriptomic response |
| title | Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia |
| title_full | Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia |
| title_fullStr | Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia |
| title_full_unstemmed | Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia |
| title_short | Microalgae Pediastrum boryanum enhances growth and modulates physiology and transcriptome in Nile tilapia |
| title_sort | microalgae pediastrum boryanum enhances growth and modulates physiology and transcriptome in nile tilapia |
| topic | Microalgae Pediastrum boryanum Nile tilapia Immunity Transcriptomic response |
| url | https://doi.org/10.1038/s41598-025-07498-1 |
| work_keys_str_mv | AT raghadesoliman microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT ahmedateya microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT mohamedabouelatta microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT wasseememam microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT mohamedmabdelrahim microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT ashrafigelhetawy microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT ahmedeelshafey microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia AT radiamohamed microalgaepediastrumboryanumenhancesgrowthandmodulatesphysiologyandtranscriptomeinniletilapia |