Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains
This study evaluated the growth performance of Tetradesmus obliquus and Chlorella vulgaris microalgae cultivated in diluted liquid digestate supplemented with CO2, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-N...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1459756/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841553384064155648 |
|---|---|
| author | Ángela Sánchez-Quintero Ángela Sánchez-Quintero Ángela Sánchez-Quintero Aurélien Parsy Amandine Adrien Amandine Adrien Lea Spitzer Javier Jiménez-Lamana Susana C. M. Fernandes Susana C. M. Fernandes Jean-Baptiste Beigbeder |
| author_facet | Ángela Sánchez-Quintero Ángela Sánchez-Quintero Ángela Sánchez-Quintero Aurélien Parsy Amandine Adrien Amandine Adrien Lea Spitzer Javier Jiménez-Lamana Susana C. M. Fernandes Susana C. M. Fernandes Jean-Baptiste Beigbeder |
| author_sort | Ángela Sánchez-Quintero |
| collection | DOAJ |
| description | This study evaluated the growth performance of Tetradesmus obliquus and Chlorella vulgaris microalgae cultivated in diluted liquid digestate supplemented with CO2, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-NH4+.L-1 combined with the continuous injection of 1% v/v CO2 enhanced the optimal growth responses and bioremediation potential for both strains in 200-mL cultures. In 6-L flat panel reactors, T. obliquus exhibited superior biomass production, achieving a final biomass concentration of 1.29 ± 0.06 g.L-1, while C. vulgaris reached only 0.36 ± 0.02 g.L-1. Both strains effectively contributed to the bioremediation of the digestate-based culture media, with up to 100% of N-NH4+, 50% of COD, and 55% of P-PO43- removals. The high nitrogen levels in the digestate-based medium significantly increased protein content, with 46.21% ± 3.98% dry weight (DW) for T. obliquus and 44.17% ± 2.24% DW for C. vulgaris as compared to the microalgae cultivated in commercial media. Additionally, the metal content of the microalgal biomass was analyzed to assess its potential use as biostimulants in compliance with European regulations. While chromium concentrations slightly exceeded regulatory thresholds in both strains, the levels of other metals remained within permissible limits. |
| format | Article |
| id | doaj-art-7091f336bee14ec1a2063ffedb24d7f6 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-7091f336bee14ec1a2063ffedb24d7f62025-01-09T10:14:56ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.14597561459756Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strainsÁngela Sánchez-Quintero0Ángela Sánchez-Quintero1Ángela Sánchez-Quintero2Aurélien Parsy3Amandine Adrien4Amandine Adrien5Lea Spitzer6Javier Jiménez-Lamana7Susana C. M. Fernandes8Susana C. M. Fernandes9Jean-Baptiste Beigbeder10APESA Pôle valorisation, Montardon, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, IPREM, CNRS, Pau, FranceMANTA—Marine Materials Research Group, Université de Pau et des Pays de l’Adour, E2S UPPA, Anglet, FranceAPESA Pôle valorisation, Montardon, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, IPREM, CNRS, Pau, FranceMANTA—Marine Materials Research Group, Université de Pau et des Pays de l’Adour, E2S UPPA, Anglet, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, IPREM, CNRS, Pau, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, IPREM, CNRS, Pau, FranceUniversité de Pau et des Pays de l’Adour, E2S UPPA, IPREM, CNRS, Pau, FranceMANTA—Marine Materials Research Group, Université de Pau et des Pays de l’Adour, E2S UPPA, Anglet, FranceAPESA Pôle valorisation, Montardon, FranceThis study evaluated the growth performance of Tetradesmus obliquus and Chlorella vulgaris microalgae cultivated in diluted liquid digestate supplemented with CO2, comparing their efficiency to that of a conventional synthetic media. The presence of an initial concentration of ammonium of 125 mg N-NH4+.L-1 combined with the continuous injection of 1% v/v CO2 enhanced the optimal growth responses and bioremediation potential for both strains in 200-mL cultures. In 6-L flat panel reactors, T. obliquus exhibited superior biomass production, achieving a final biomass concentration of 1.29 ± 0.06 g.L-1, while C. vulgaris reached only 0.36 ± 0.02 g.L-1. Both strains effectively contributed to the bioremediation of the digestate-based culture media, with up to 100% of N-NH4+, 50% of COD, and 55% of P-PO43- removals. The high nitrogen levels in the digestate-based medium significantly increased protein content, with 46.21% ± 3.98% dry weight (DW) for T. obliquus and 44.17% ± 2.24% DW for C. vulgaris as compared to the microalgae cultivated in commercial media. Additionally, the metal content of the microalgal biomass was analyzed to assess its potential use as biostimulants in compliance with European regulations. While chromium concentrations slightly exceeded regulatory thresholds in both strains, the levels of other metals remained within permissible limits.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1459756/fullmicroalgaeliquid digestateCO2market regulationbiostimulantsbiomass composition |
| spellingShingle | Ángela Sánchez-Quintero Ángela Sánchez-Quintero Ángela Sánchez-Quintero Aurélien Parsy Amandine Adrien Amandine Adrien Lea Spitzer Javier Jiménez-Lamana Susana C. M. Fernandes Susana C. M. Fernandes Jean-Baptiste Beigbeder Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains Frontiers in Bioengineering and Biotechnology microalgae liquid digestate CO2 market regulation biostimulants biomass composition |
| title | Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains |
| title_full | Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains |
| title_fullStr | Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains |
| title_full_unstemmed | Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains |
| title_short | Effects of CO2 and liquid digestate concentrations on the growth performance and biomass composition of Tetradesmus obliquus and Chlorella vulgaris microalgal strains |
| title_sort | effects of co2 and liquid digestate concentrations on the growth performance and biomass composition of tetradesmus obliquus and chlorella vulgaris microalgal strains |
| topic | microalgae liquid digestate CO2 market regulation biostimulants biomass composition |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1459756/full |
| work_keys_str_mv | AT angelasanchezquintero effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT angelasanchezquintero effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT angelasanchezquintero effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT aurelienparsy effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT amandineadrien effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT amandineadrien effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT leaspitzer effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT javierjimenezlamana effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT susanacmfernandes effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT susanacmfernandes effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains AT jeanbaptistebeigbeder effectsofco2andliquiddigestateconcentrationsonthegrowthperformanceandbiomasscompositionoftetradesmusobliquusandchlorellavulgarismicroalgalstrains |