Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage

Abstract The extensive use of glyphosate, while effective in weed control, poses significant risks to non-target plant species such as Handroanthus albus (yellow Ipe), an important species in tropical sustainable forestry. This study aimed to assess the impact of glyphosate on young Ipe plants and t...

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Main Authors: Milton Garcia Costa, Rafael de Amorim Cordeiro, Júlia Karoline Rodrigues das Mercês, Liedson Silva de Medeiros, Alexandre Moraes Cardoso, Renato de Mello Prado, Davie M. Kadyampakeni, Maria Thalia Lacerda Siqueira, Jonas Pereira de Souza Junior
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-07527-z
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author Milton Garcia Costa
Rafael de Amorim Cordeiro
Júlia Karoline Rodrigues das Mercês
Liedson Silva de Medeiros
Alexandre Moraes Cardoso
Renato de Mello Prado
Davie M. Kadyampakeni
Maria Thalia Lacerda Siqueira
Jonas Pereira de Souza Junior
author_facet Milton Garcia Costa
Rafael de Amorim Cordeiro
Júlia Karoline Rodrigues das Mercês
Liedson Silva de Medeiros
Alexandre Moraes Cardoso
Renato de Mello Prado
Davie M. Kadyampakeni
Maria Thalia Lacerda Siqueira
Jonas Pereira de Souza Junior
author_sort Milton Garcia Costa
collection DOAJ
description Abstract The extensive use of glyphosate, while effective in weed control, poses significant risks to non-target plant species such as Handroanthus albus (yellow Ipe), an important species in tropical sustainable forestry. This study aimed to assess the impact of glyphosate on young Ipe plants and to investigate the protective role of silicon (Si) supplementation. Increasing glyphosate concentrations were found to induce heightened cellular electrolyte leakage and reduced concentrations of photosynthetic pigments in young Ipe leaves. Glyphosate exposure also compromised photosynthetic efficiency, resulting in decreased leaf biomass production. Conversely, supplemental applications of Si, applied via root and foliar routes, significantly increased Si accumulation in young Ipe leaves and mitigated the adverse effects of glyphosate. Silicon treatment decreased electrolyte cellular leakage by enhancing antioxidant defenses, particularly through elevated flavonoid and anthocyanin levels, and preserved photosynthetic efficiency. Si-treated plants maintained higher chlorophyll a concentration and exhibited improved photochemical efficiency, even under moderate rates of glyphosate stress. Consequently, Si application led to increased leaf dry mass, particularly at moderate glyphosate concentrations, highlighting its role in enhancing the resilience of young Ipe plants to moderate herbicide-induced stress. Incorporating Si into sustainable forestry practices could enhance the resilience of key species, supporting reforestation and afforestation efforts in glyphosate-prone environments.
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spelling doaj-art-7071cde2947e4da09bac578cda4f18dd2025-08-20T03:45:30ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-07527-zSilicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakageMilton Garcia Costa0Rafael de Amorim Cordeiro1Júlia Karoline Rodrigues das Mercês2Liedson Silva de Medeiros3Alexandre Moraes Cardoso4Renato de Mello Prado5Davie M. Kadyampakeni6Maria Thalia Lacerda Siqueira7Jonas Pereira de Souza Junior8Department of Soil Science, School of Agricultural and Veterinary Sciences, São Paulo State University ‘Júlio de Mesquita Filho’Federal Institute of Education, Science and Technology of Sao PauloDepartment of Biology, School of Agricultural and Veterinary Sciences, São Paulo State University ‘Júlio de Mesquita Filho’Federal Institute of Education, Science and Technology of Sao PauloFederal Institute of Education, Science and Technology of Sao PauloDepartment of Soil Science, School of Agricultural and Veterinary Sciences, São Paulo State University ‘Júlio de Mesquita Filho’Citrus Research and Education Center, University of FloridaDepartment of Plant Protection, School of Agricultural and Veterinary Sciences, São Paulo State University ‘Júlio de Mesquita Filho’Citrus Research and Education Center, University of FloridaAbstract The extensive use of glyphosate, while effective in weed control, poses significant risks to non-target plant species such as Handroanthus albus (yellow Ipe), an important species in tropical sustainable forestry. This study aimed to assess the impact of glyphosate on young Ipe plants and to investigate the protective role of silicon (Si) supplementation. Increasing glyphosate concentrations were found to induce heightened cellular electrolyte leakage and reduced concentrations of photosynthetic pigments in young Ipe leaves. Glyphosate exposure also compromised photosynthetic efficiency, resulting in decreased leaf biomass production. Conversely, supplemental applications of Si, applied via root and foliar routes, significantly increased Si accumulation in young Ipe leaves and mitigated the adverse effects of glyphosate. Silicon treatment decreased electrolyte cellular leakage by enhancing antioxidant defenses, particularly through elevated flavonoid and anthocyanin levels, and preserved photosynthetic efficiency. Si-treated plants maintained higher chlorophyll a concentration and exhibited improved photochemical efficiency, even under moderate rates of glyphosate stress. Consequently, Si application led to increased leaf dry mass, particularly at moderate glyphosate concentrations, highlighting its role in enhancing the resilience of young Ipe plants to moderate herbicide-induced stress. Incorporating Si into sustainable forestry practices could enhance the resilience of key species, supporting reforestation and afforestation efforts in glyphosate-prone environments.https://doi.org/10.1038/s41598-025-07527-zBeneficial elementHerbicide stressStress Attenuation
spellingShingle Milton Garcia Costa
Rafael de Amorim Cordeiro
Júlia Karoline Rodrigues das Mercês
Liedson Silva de Medeiros
Alexandre Moraes Cardoso
Renato de Mello Prado
Davie M. Kadyampakeni
Maria Thalia Lacerda Siqueira
Jonas Pereira de Souza Junior
Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
Scientific Reports
Beneficial element
Herbicide stress
Stress Attenuation
title Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
title_full Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
title_fullStr Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
title_full_unstemmed Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
title_short Silicon can attenuate glyphosate-induced stress in young Handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
title_sort silicon can attenuate glyphosate induced stress in young handroanthus albus by improving photosynthetic efficiency and decreasing cellular electrolyte leakage
topic Beneficial element
Herbicide stress
Stress Attenuation
url https://doi.org/10.1038/s41598-025-07527-z
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