Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling
Abstract Finger millet blast caused by Pyricularia grisea hinders crop’s growth and is a serious threat to economic yield. It can lead to massive yield losses i.e. up to 80%, which is a cause of major concern. In this study, we evaluated 200 accessions of finger millet at Banda University of Agricul...
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Nature Portfolio
2024-12-01
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| author | Isha Ojha Kamaluddin Vijay Sharma Mohd. Tariq Mukul Jain Hoshiyar Singh Negi Mukul Kumar Shalesh Kumar Singh Nitin Kumar Vijay Kumar Kushwaha Prashant Kaushik Kirtan Dave |
| author_facet | Isha Ojha Kamaluddin Vijay Sharma Mohd. Tariq Mukul Jain Hoshiyar Singh Negi Mukul Kumar Shalesh Kumar Singh Nitin Kumar Vijay Kumar Kushwaha Prashant Kaushik Kirtan Dave |
| author_sort | Isha Ojha |
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| description | Abstract Finger millet blast caused by Pyricularia grisea hinders crop’s growth and is a serious threat to economic yield. It can lead to massive yield losses i.e. up to 80%, which is a cause of major concern. In this study, we evaluated 200 accessions of finger millet at Banda University of Agriculture & Technology in Banda, India, under natural conditions to determine their resistance against leaf, neck, & finger blast diseases. Nine accessions displayed strong resistance against all forms of blast. The genetic variability analysis revealed high phenotypic & genotypic coefficients of variation. This was especially true for seed yield, plant height, & days to flowering. Most traits had heritability estimates exceeding 80%. Such results indicate a significant potential for effective selection in breeding programs. Leaf blast ratings had a significant but weaker correlation to both neck blast (r = 0.31) and finger blast (r = 0.31) whereas neck and finger blast ratings were very highly correlated and significant (r = 0.97). All types of blast had negative correlation with various agronomic traits studied. Furthermore, DNA fingerprinting of resistant genotypes with SSR markers displayed 10 polymorphic markers with an average of 2.6 alleles per locus. This points to the genetic diversity present within the germplasm. In summary, these findings provide vital insights for breeding initiatives focused on enhancing blast resistance in finger millet. Such efforts are crucial for maintaining the sustainability of this important crop for global food security. |
| format | Article |
| id | doaj-art-706caa31cdab4e679015f0ac0bd13e6a |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-706caa31cdab4e679015f0ac0bd13e6a2025-01-05T12:27:41ZengNature PortfolioScientific Reports2045-23222024-12-0114111510.1038/s41598-024-83797-3Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profilingIsha Ojha0Kamaluddin1Vijay Sharma2Mohd. Tariq3Mukul Jain4Hoshiyar Singh Negi5Mukul Kumar6Shalesh Kumar Singh7Nitin Kumar8Vijay Kumar Kushwaha9Prashant Kaushik10Kirtan Dave11Department of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyParul Institute of Applied Sciences, Parul UniversityParul Institute of Applied Sciences, Parul UniversityDepartment of Plant Pathology, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Banda University of Agriculture and TechnologyDepartment of Genetics and Plant Breeding, Acharya Narendra Deva University of Agriculture and TechnologyChaudhary Charan Singh Haryana Agriculture UniversityBioinformatics Laboratory, Research & Developmental Cell, Parul UniversityAbstract Finger millet blast caused by Pyricularia grisea hinders crop’s growth and is a serious threat to economic yield. It can lead to massive yield losses i.e. up to 80%, which is a cause of major concern. In this study, we evaluated 200 accessions of finger millet at Banda University of Agriculture & Technology in Banda, India, under natural conditions to determine their resistance against leaf, neck, & finger blast diseases. Nine accessions displayed strong resistance against all forms of blast. The genetic variability analysis revealed high phenotypic & genotypic coefficients of variation. This was especially true for seed yield, plant height, & days to flowering. Most traits had heritability estimates exceeding 80%. Such results indicate a significant potential for effective selection in breeding programs. Leaf blast ratings had a significant but weaker correlation to both neck blast (r = 0.31) and finger blast (r = 0.31) whereas neck and finger blast ratings were very highly correlated and significant (r = 0.97). All types of blast had negative correlation with various agronomic traits studied. Furthermore, DNA fingerprinting of resistant genotypes with SSR markers displayed 10 polymorphic markers with an average of 2.6 alleles per locus. This points to the genetic diversity present within the germplasm. In summary, these findings provide vital insights for breeding initiatives focused on enhancing blast resistance in finger millet. Such efforts are crucial for maintaining the sustainability of this important crop for global food security.https://doi.org/10.1038/s41598-024-83797-3Finger milletBlast diseaseResistanceDNA fingerprintingSSR markers |
| spellingShingle | Isha Ojha Kamaluddin Vijay Sharma Mohd. Tariq Mukul Jain Hoshiyar Singh Negi Mukul Kumar Shalesh Kumar Singh Nitin Kumar Vijay Kumar Kushwaha Prashant Kaushik Kirtan Dave Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling Scientific Reports Finger millet Blast disease Resistance DNA fingerprinting SSR markers |
| title | Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling |
| title_full | Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling |
| title_fullStr | Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling |
| title_full_unstemmed | Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling |
| title_short | Identification of blast resistant finger millet (Eleusine coracana L.) genotype through phenotypic screening and molecular profiling |
| title_sort | identification of blast resistant finger millet eleusine coracana l genotype through phenotypic screening and molecular profiling |
| topic | Finger millet Blast disease Resistance DNA fingerprinting SSR markers |
| url | https://doi.org/10.1038/s41598-024-83797-3 |
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