Environmental Pollutant Anthracene Induces ABA-Dependent Transgenerational Effects on Gemmae Dormancy in <i>Marchantia polymorpha</i>

Anthracene, a polycyclic aromatic hydrocarbon (PAH) from fossil fuel combustion, poses significant environmental threats. This study investigates the role of abscisic acid (ABA) in the anthracene tolerance of the liverwort <i>Marchantia polymorpha</i> using mutants deficient in ABA perce...

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Main Authors: Juan I. Tolopka, Maya Svriz, Tamara M. Ledesma, Eugenia Lanari, José M. Scervino, Javier E. Moreno
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/13/21/2979
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Summary:Anthracene, a polycyclic aromatic hydrocarbon (PAH) from fossil fuel combustion, poses significant environmental threats. This study investigates the role of abscisic acid (ABA) in the anthracene tolerance of the liverwort <i>Marchantia polymorpha</i> using mutants deficient in ABA perception (Mp<i>pyl1</i>) or biosynthesis (Mp<i>aba1</i>). In this study, we monitored the role of ABA in the anthracene tolerance response by tracking two ABA-controlled traits: plant growth inhibition and gemmae dormancy. We found that the anthracene-induced inhibition of plant growth is dose-dependent, similar to the growth-inhibiting effect of ABA, but independent of ABA pathways. However, gemmae dormancy was differentially affected by anthracene in ABA-deficient mutants. We found that gemmae from anthracene-exposed WT plants exhibited reduced germination compared to those from mock-treated plants. This suggests that the anthracene exposure of mother plants induces a transgenerational effect, resulting in prolonged dormancy in their asexual propagules. While Mp<i>pyl1</i> gemmae retained a dormancy delay when derived from anthracene-exposed thalli, the ABA biosynthesis mutant Mp<i>aba1</i> did not display any significant dormancy delay as a consequence of anthracene exposure. These results, together with the strong induction of ABA marker genes upon anthracene treatment, imply that anthracene-induced germination inhibition relies on ABA synthesis in the mother plant, highlighting the critical role of MpABA1 in the tolerance response. These findings reveal a complex interplay between anthracene stress and ABA signaling, where anthracene triggers ABA-mediated responses, influencing reproductive success and highlighting the potential for leveraging genetic and hormonal pathways to enhance plant resilience in contaminated habitats.
ISSN:2223-7747