Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum
Microbial inoculants offer an environmentally friendly approach to enhance plant growth and control disease. In this study, two Bacillus velezensis strains, HKSSLJEBR3 (R3) and Ya-1 were isolated from pepper plant roots and tropical rainforest soil, respectively. Both strains exhibited strong antifu...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504660/full |
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| author | Bao Wang Bao Wang Bao Wang Shimeng Tan Mingde Wu Yujie Feng Wanrong Yan Qian Yun Xuncong Ji Runmao Lin Zhixiang Zhao |
| author_facet | Bao Wang Bao Wang Bao Wang Shimeng Tan Mingde Wu Yujie Feng Wanrong Yan Qian Yun Xuncong Ji Runmao Lin Zhixiang Zhao |
| author_sort | Bao Wang |
| collection | DOAJ |
| description | Microbial inoculants offer an environmentally friendly approach to enhance plant growth and control disease. In this study, two Bacillus velezensis strains, HKSSLJEBR3 (R3) and Ya-1 were isolated from pepper plant roots and tropical rainforest soil, respectively. Both strains exhibited strong antifungal activity against Fusarium oxysporum f. sp. capsici, with inhibition rates of 48.54 ± 0.66% for R3 and 49.35 ± 1.44% for Ya-1. In greenhouse trials, R3 significantly boosted pepper growth, with a 22.12% increase in plant height, 46.44% more leaves, and 56.29% greater fresh weight. These enhancements were likely due to the strong affinity between R3 and pepper plants. Both strains also improved soil quality, with R3 increasing available potassium (AK) by 5.13% and soil organic matter (SOM) by 4.03%, while Ya-1 showed more significant increases. Metagenomic analysis revealed that both strains altered the rhizosphere microbiome, with R3 promoting Pseudomonas and suppressing Fusarium. These results suggest that the R3 strain has strong potential for enhancing pepper growth, improving soil health, and reshaping the rhizosphere microbiome. |
| format | Article |
| id | doaj-art-6ff660b498a24b01913704b368cda3f5 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-6ff660b498a24b01913704b368cda3f52024-12-09T06:28:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-12-011510.3389/fmicb.2024.15046601504660Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annumBao Wang0Bao Wang1Bao Wang2Shimeng Tan3Mingde Wu4Yujie Feng5Wanrong Yan6Qian Yun7Xuncong Ji8Runmao Lin9Zhixiang Zhao10Key Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Haikou and Sanya, ChinaSchool of Life and Health Science, Hainan University, Haikou, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, School of Tropical Agriculture and Forestry, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Haikou and Sanya, ChinaKey Laboratory of Plant Disease and Pest Control of Hainan Province, Institute of Plant Protection Academy of Agricultural Sciences (Research Center of Quality Safety and Standards for Agricultural Products of Hainan Academy of Agricultural Sciences), Haikou, ChinaMicrobial inoculants offer an environmentally friendly approach to enhance plant growth and control disease. In this study, two Bacillus velezensis strains, HKSSLJEBR3 (R3) and Ya-1 were isolated from pepper plant roots and tropical rainforest soil, respectively. Both strains exhibited strong antifungal activity against Fusarium oxysporum f. sp. capsici, with inhibition rates of 48.54 ± 0.66% for R3 and 49.35 ± 1.44% for Ya-1. In greenhouse trials, R3 significantly boosted pepper growth, with a 22.12% increase in plant height, 46.44% more leaves, and 56.29% greater fresh weight. These enhancements were likely due to the strong affinity between R3 and pepper plants. Both strains also improved soil quality, with R3 increasing available potassium (AK) by 5.13% and soil organic matter (SOM) by 4.03%, while Ya-1 showed more significant increases. Metagenomic analysis revealed that both strains altered the rhizosphere microbiome, with R3 promoting Pseudomonas and suppressing Fusarium. These results suggest that the R3 strain has strong potential for enhancing pepper growth, improving soil health, and reshaping the rhizosphere microbiome.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504660/fullBacillus velezensisantifungal activitybiological controlplant growth-promoting microorganismssoil rhizosphere communitymetagenomic |
| spellingShingle | Bao Wang Bao Wang Bao Wang Shimeng Tan Mingde Wu Yujie Feng Wanrong Yan Qian Yun Xuncong Ji Runmao Lin Zhixiang Zhao Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum Frontiers in Microbiology Bacillus velezensis antifungal activity biological control plant growth-promoting microorganisms soil rhizosphere community metagenomic |
| title | Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum |
| title_full | Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum |
| title_fullStr | Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum |
| title_full_unstemmed | Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum |
| title_short | Effects of two Bacillus velezensis strains isolated from different sources on the growth of Capsicum annum |
| title_sort | effects of two bacillus velezensis strains isolated from different sources on the growth of capsicum annum |
| topic | Bacillus velezensis antifungal activity biological control plant growth-promoting microorganisms soil rhizosphere community metagenomic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1504660/full |
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