Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities
Aqueous extracts of Leea crispa used for producing silver nanoparticles (AgNPs) with the supplementation of the external capping substance, were determined by UV-Visible spectroscopy. The synthesized nanoparticles were examined for their antituberculosis and anticancer activities. The presence of ph...
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North Carolina State University
2024-11-01
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| Online Access: | https://ojs.bioresources.com/index.php/BRJ/article/view/23428 |
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| author | Tejshwini Doddamani Balamuralikrishnan Balasubramanian Siddappa Kakkalameli Joseph Kadanthottu Sebastian Manikantan Pappuswamy Bader O. Almutairi Mikhlid H. Almutairi Azharuddin Daphedar |
| author_facet | Tejshwini Doddamani Balamuralikrishnan Balasubramanian Siddappa Kakkalameli Joseph Kadanthottu Sebastian Manikantan Pappuswamy Bader O. Almutairi Mikhlid H. Almutairi Azharuddin Daphedar |
| author_sort | Tejshwini Doddamani |
| collection | DOAJ |
| description | Aqueous extracts of Leea crispa used for producing silver nanoparticles (AgNPs) with the supplementation of the external capping substance, were determined by UV-Visible spectroscopy. The synthesized nanoparticles were examined for their antituberculosis and anticancer activities. The presence of phytoconstituents available for reducing silver ions and to form the AgNPs was confirmed using FTIR analysis. The XRD and TEM examination validated the AgNPs’ spherical particle shape and size of 15 to 85 nm and their face-centered cubic crystal form. Additionally, the FTIR spectrum revealed variation in the band values in the range 1384.0 to 3419.4 cm-1, respectively, and the EDX noted a strong band at 3 keV induced the presence of metallic silver. The AgNPs exhibited comparatively potential anti-tuberculosis activity (0.2 to 100 µg/mL) respectively. Alternatively, various doses of AgNPs, 12.5 to 400 µg/mL documented considerable activity towards the human breast cancer cell lines. The percentage of cell viability increased at 12.5g/mL and declined at 400 ng/mL concentrations of AgNPs solution. The AgNPs synthesized from L. crispa exhibited potential activity against life-threatening tuberculosis and cancer cells. |
| format | Article |
| id | doaj-art-6fab6fd18a1b493f93e48e8875d2429b |
| institution | Kabale University |
| issn | 1930-2126 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | North Carolina State University |
| record_format | Article |
| series | BioResources |
| spelling | doaj-art-6fab6fd18a1b493f93e48e8875d2429b2025-01-02T18:15:18ZengNorth Carolina State UniversityBioResources1930-21262024-11-012014524641580Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer ActivitiesTejshwini Doddamani0Balamuralikrishnan Balasubramanian1https://orcid.org/0000-0001-6938-1495Siddappa Kakkalameli2Joseph Kadanthottu Sebastian3https://orcid.org/0000-0003-4384-2462Manikantan Pappuswamy4Bader O. Almutairi5Mikhlid H. Almutairi6Azharuddin Daphedar7Department of Botany, Environmental Biology Laboratory, Karnatak University, Dharwad, Karnataka-580 003, IndiaDepartment of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul 05006, South KoreaDepartment of Botany, Environmental Biology Laboratory, Davangere University, Davangere, Karnataka India, 577002Department of Life Sciences, Christ University, Bengaluru, Karnataka, India-560029Department of Life Sciences, Christ University, Bengaluru, Karnataka, India-560029Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, Environmental Biology Laboratory, Karnatak University, Dharwad, Karnataka- 580 003, India; Department of Botany, Anjuman Arts, Science and Commerce College, Vijayapur-586 101, Karnataka. IndiaAqueous extracts of Leea crispa used for producing silver nanoparticles (AgNPs) with the supplementation of the external capping substance, were determined by UV-Visible spectroscopy. The synthesized nanoparticles were examined for their antituberculosis and anticancer activities. The presence of phytoconstituents available for reducing silver ions and to form the AgNPs was confirmed using FTIR analysis. The XRD and TEM examination validated the AgNPs’ spherical particle shape and size of 15 to 85 nm and their face-centered cubic crystal form. Additionally, the FTIR spectrum revealed variation in the band values in the range 1384.0 to 3419.4 cm-1, respectively, and the EDX noted a strong band at 3 keV induced the presence of metallic silver. The AgNPs exhibited comparatively potential anti-tuberculosis activity (0.2 to 100 µg/mL) respectively. Alternatively, various doses of AgNPs, 12.5 to 400 µg/mL documented considerable activity towards the human breast cancer cell lines. The percentage of cell viability increased at 12.5g/mL and declined at 400 ng/mL concentrations of AgNPs solution. The AgNPs synthesized from L. crispa exhibited potential activity against life-threatening tuberculosis and cancer cells.https://ojs.bioresources.com/index.php/BRJ/article/view/23428leea crispatemagno3mtt assaymcf-7 cell lineanti-cancer anti-tuberculosis |
| spellingShingle | Tejshwini Doddamani Balamuralikrishnan Balasubramanian Siddappa Kakkalameli Joseph Kadanthottu Sebastian Manikantan Pappuswamy Bader O. Almutairi Mikhlid H. Almutairi Azharuddin Daphedar Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities BioResources leea crispa tem agno3 mtt assay mcf-7 cell line anti-cancer anti-tuberculosis |
| title | Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities |
| title_full | Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities |
| title_fullStr | Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities |
| title_full_unstemmed | Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities |
| title_short | Phytofabricated Silver Nanoparticles Derived from Leea crispa Leaf Extract: Antituberculosis and Anticancer Activities |
| title_sort | phytofabricated silver nanoparticles derived from leea crispa leaf extract antituberculosis and anticancer activities |
| topic | leea crispa tem agno3 mtt assay mcf-7 cell line anti-cancer anti-tuberculosis |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/23428 |
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