Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line
The pharmaceutical industry has long focused on nature-derived biologically active compounds to address challenges in cancer treatment, particularly overcoming cancer cell resistance to programmed cell death, including apoptosis and ferroptosis. The cytotoxic effect of Amanita muscaria acetone extra...
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| Format: | Article |
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University of Kragujevac, Faculty of Science
2024-01-01
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| Series: | Kragujevac Journal of Science |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362401121M.pdf |
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| author | Milojević Sara D. Nikodijević Danijela D. Milutinović Milena G. Kosanić Marijana M. Petrović Nevena N. Jovanović-Krivokuća Milica G. Nacka-Aleksić Mirjana M. |
| author_facet | Milojević Sara D. Nikodijević Danijela D. Milutinović Milena G. Kosanić Marijana M. Petrović Nevena N. Jovanović-Krivokuća Milica G. Nacka-Aleksić Mirjana M. |
| author_sort | Milojević Sara D. |
| collection | DOAJ |
| description | The pharmaceutical industry has long focused on nature-derived biologically active compounds to address challenges in cancer treatment, particularly overcoming cancer cell resistance to programmed cell death, including apoptosis and ferroptosis. The cytotoxic effect of Amanita muscaria acetone extract on the JAR choriocarcinoma cell line was evaluated using MTT assay and analysis on two selected programmed cell death pathways. The putative ferroptotic effect was evaluated by measuring the main redox parameters and relative expression of key antiferroptotic genes. Our findings indicate that the IC50 of the acetone extract of A. muscaria was 168.96 (after 24 h) and 43.85 (after 72 h) mg/mL, inducing mainly a proapoptotic effect on choriocarcinoma cells. The extract also demonstrated an antiferroptotic effect. Both the proapoptotic and antiferroptotic effects were achieved regardless of the induction of oxidative stress._ This is the first report on A. muscaria acetone extract indicating proapoptotic and antiferroptotic effects on choriocarcinoma cells. |
| format | Article |
| id | doaj-art-6fa3b02fed0d4663a30822dfd5f91403 |
| institution | Kabale University |
| issn | 1450-9636 2466-5509 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | University of Kragujevac, Faculty of Science |
| record_format | Article |
| series | Kragujevac Journal of Science |
| spelling | doaj-art-6fa3b02fed0d4663a30822dfd5f914032025-01-08T16:26:47ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092024-01-0146112113510.5937/KgJSci2401121M1450-96362401121MExploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell lineMilojević Sara D.0https://orcid.org/0000-0002-9755-2762Nikodijević Danijela D.1https://orcid.org/0000-0002-6453-0313Milutinović Milena G.2https://orcid.org/0000-0002-5075-5650Kosanić Marijana M.3https://orcid.org/0000-0002-5714-7631Petrović Nevena N.4https://orcid.org/0000-0002-7983-0597Jovanović-Krivokuća Milica G.5https://orcid.org/0000-0001-8292-4853Nacka-Aleksić Mirjana M.6https://orcid.org/0000-0003-0750-9347University of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Science, Department of Biology and Ecology, Kragujevac, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Belgrade, SerbiaUniversity of Belgrade, Institute for the Application of Nuclear Energy (INEP), Department for Biology of Reproduction, Belgrade, SerbiaThe pharmaceutical industry has long focused on nature-derived biologically active compounds to address challenges in cancer treatment, particularly overcoming cancer cell resistance to programmed cell death, including apoptosis and ferroptosis. The cytotoxic effect of Amanita muscaria acetone extract on the JAR choriocarcinoma cell line was evaluated using MTT assay and analysis on two selected programmed cell death pathways. The putative ferroptotic effect was evaluated by measuring the main redox parameters and relative expression of key antiferroptotic genes. Our findings indicate that the IC50 of the acetone extract of A. muscaria was 168.96 (after 24 h) and 43.85 (after 72 h) mg/mL, inducing mainly a proapoptotic effect on choriocarcinoma cells. The extract also demonstrated an antiferroptotic effect. Both the proapoptotic and antiferroptotic effects were achieved regardless of the induction of oxidative stress._ This is the first report on A. muscaria acetone extract indicating proapoptotic and antiferroptotic effects on choriocarcinoma cells.https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362401121M.pdfamanita muscariamedicinal mushroomscancer treatmentapoptosisferroptosisjar choriocarcinoma cell line |
| spellingShingle | Milojević Sara D. Nikodijević Danijela D. Milutinović Milena G. Kosanić Marijana M. Petrović Nevena N. Jovanović-Krivokuća Milica G. Nacka-Aleksić Mirjana M. Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line Kragujevac Journal of Science amanita muscaria medicinal mushrooms cancer treatment apoptosis ferroptosis jar choriocarcinoma cell line |
| title | Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line |
| title_full | Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line |
| title_fullStr | Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line |
| title_full_unstemmed | Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line |
| title_short | Exploring Amanita muscaria (L.) Lam.: Cytotoxic, proapoptotic and antiferroptotic effect of acetone extract on JAR choriocarcinoma cell line |
| title_sort | exploring amanita muscaria l lam cytotoxic proapoptotic and antiferroptotic effect of acetone extract on jar choriocarcinoma cell line |
| topic | amanita muscaria medicinal mushrooms cancer treatment apoptosis ferroptosis jar choriocarcinoma cell line |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2024/1450-96362401121M.pdf |
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