Determination of alpha-napthyl acetate esterase activity of native and frozen-thawed bull sperm acrosome
<b>Aim: </b>The assay of the leakage of acrosomal enzymes into seminal plasma might provide an early and sensitive evidence for membrane damage ocurred during freezing of semen for artificial insemination. Alpha-naphthyl acetate esterase is one of the acrosomal enzymes that can be used f...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2020-03-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1271 |
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| Summary: | <b>Aim: </b>The assay of the leakage of acrosomal enzymes into seminal plasma
might provide an early and sensitive evidence for membrane damage ocurred
during freezing of semen for artificial insemination. Alpha-naphthyl acetate
esterase is one of the acrosomal enzymes that can be used for this purpose.
In the the present study, the positivity rates and localization pattern of alphanaphthyl
acetate esterase in the spermatozoa of both native and frozen-thawed
bull semen samples were determined by light microscopy.<p>
<b>Materials and Methods:</b> Semen samples of 7 bull were used as material. Smears
were prepared from native and frozen-thawed semen samples. The enzyme
was demonstrated by a cytochemical method. Specimens were observed
under ligth microscope. Positivity rates of alpha-naphtyl acetate eseterasepositive
spermatozoa, distribution and localization pattern of the enzymatic
reaction were determined.<p>
<b>Results: </b>The reddish-brown enzymatic reaction was peculiar to spermatozoon
acrosome. In some cells, the enzymatic reaction was observed as a narrow
band located on acrosomal membrane. The enzymatic reaction intesity was
not similar in all cells and was weaker in some cells. The reaction product
primarily located in the acrosome. There were large individual differences in
alpha-naphthyl acetate esterase positivity rates of the native sperm samples.
The native semen samples with low spermatozoon vitality and motility tended
to display lower of alpha-naphthyl acetate esterase positivity rates. Freeze-
thawing process significantly decreased alpha-naphthyl acetate esterase
positivity rates of the samples.<p>
<b>Conclusion: </b>Acrosomal alpha-naphtyl acetate esterase might be considered
as a significant parameter in the asssessment of availibility for freezing and to
determine fertility potential of the bull semen. |
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| ISSN: | 1309-6958 2146-1953 |