Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo
Hyperuricemia (HUA) is a vital risk factor for chronic kidney diseases (CKD) and development of functional foods capable of protecting CKD is of importance. This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides (ADBP) on HUA-induced kidney damage. In...
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| Format: | Article |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250157 |
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| author | Wei Li Haihong Chen Hongliang Chen Ziyin Li Wei Hu Qinxuan Zhou Bing Xu Yi Wang Xinhui Xing |
| author_facet | Wei Li Haihong Chen Hongliang Chen Ziyin Li Wei Hu Qinxuan Zhou Bing Xu Yi Wang Xinhui Xing |
| author_sort | Wei Li |
| collection | DOAJ |
| description | Hyperuricemia (HUA) is a vital risk factor for chronic kidney diseases (CKD) and development of functional foods capable of protecting CKD is of importance. This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides (ADBP) on HUA-induced kidney damage. In the present study, we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents. In vitro results found that ADBP protected uric acid (UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense. In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice, evidenced by a remarkable decrease in serum UA, creatinine and blood urea nitrogen, improving kidney UA excretion, antioxidant defense and histological kidney deterioration. Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction. Intriguingly, ADBP restored the gut microbiome homeostasis in CKD mice, especially with respect to the elevated helpful microbial abundance, and the decreased harmful bacterial abundance. This study demonstrated that ADBP displayed great nephroprotective effects, and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD. |
| format | Article |
| id | doaj-art-6f32f6570dc5492f9f2d1da5178e1eeb |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-6f32f6570dc5492f9f2d1da5178e1eeb2025-01-10T06:56:47ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011341886190510.26599/FSHW.2022.9250157Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivoWei Li0Haihong Chen1Hongliang Chen2Ziyin Li3Wei Hu4Qinxuan Zhou5Bing Xu6Yi Wang7Xinhui Xing8Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaAffiliated Dongguan Hospital, Southern Medical University (Dongguan People’s Hospital), Dongguan 523059, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaKey Laboratory for Industrial Biocatalysis, Ministry of Education, Institute of Biochemical Engineering, Department of Chemical Engineering, Beijing 100084, ChinaInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, ChinaHyperuricemia (HUA) is a vital risk factor for chronic kidney diseases (CKD) and development of functional foods capable of protecting CKD is of importance. This paper aimed to explore the amelioration effects and mechanism of Andrias davidianus bone peptides (ADBP) on HUA-induced kidney damage. In the present study, we generated the standard ADBP which contained high hydrophobic amino acid and low molecular peptide contents. In vitro results found that ADBP protected uric acid (UA)-induced HK-2 cells from damage by modulating urate transporters and antioxidant defense. In vivo results indicated that ADBP effectively ameliorated renal injury in HUA-induced CKD mice, evidenced by a remarkable decrease in serum UA, creatinine and blood urea nitrogen, improving kidney UA excretion, antioxidant defense and histological kidney deterioration. Metabolomic analysis highlighted 14 metabolites that could be selected as potential biomarkers and attributed to the amelioration effects of ADBP on CKD mice kidney dysfunction. Intriguingly, ADBP restored the gut microbiome homeostasis in CKD mice, especially with respect to the elevated helpful microbial abundance, and the decreased harmful bacterial abundance. This study demonstrated that ADBP displayed great nephroprotective effects, and has great promise as a food or functional food ingredient for the prevention and treatment of HUA-induced CKD.https://www.sciopen.com/article/10.26599/FSHW.2022.9250157andrias davidianus bone peptideshyperuricemiauric acidchronic kidney diseasegut microbiota |
| spellingShingle | Wei Li Haihong Chen Hongliang Chen Ziyin Li Wei Hu Qinxuan Zhou Bing Xu Yi Wang Xinhui Xing Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo Food Science and Human Wellness andrias davidianus bone peptides hyperuricemia uric acid chronic kidney disease gut microbiota |
| title | Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo |
| title_full | Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo |
| title_fullStr | Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo |
| title_full_unstemmed | Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo |
| title_short | Andrias davidianus bone peptides alleviates hyperuricemia-induced kidney damage in vitro and in vivo |
| title_sort | andrias davidianus bone peptides alleviates hyperuricemia induced kidney damage in vitro and in vivo |
| topic | andrias davidianus bone peptides hyperuricemia uric acid chronic kidney disease gut microbiota |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250157 |
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