On the extension of the use of a standard operating procedure for nicotine, glycerol and propylene glycol analysis in e-liquids using mass spectrometry
Introduction Standard operating procedures (SOP), accessible to several laboratories, are essential for product verification. EU-JATC (European-Joint Action on Tobacco Control) SOP and the WHO TobLabNet (World Health Organization Tobacco Laboratories Network) SOP (SOP11) are available standard metho...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
European Publishing
2024-09-01
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| Series: | Tobacco Induced Diseases |
| Subjects: | |
| Online Access: | https://www.tobaccoinduceddiseases.org/On-the-extension-of-the-use-of-a-standard-operating-procedure-for-nicotine-glycerol,191823,0,2.html |
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| Summary: | Introduction
Standard operating procedures (SOP), accessible to several
laboratories, are essential for product verification. EU-JATC (European-Joint
Action on Tobacco Control) SOP and the WHO TobLabNet (World Health
Organization Tobacco Laboratories Network) SOP (SOP11) are available standard
methodologies to measure nicotine, glycerol, and propylene glycol, and propose
mass spectrometer (MS) as an alternative method to flame ionization detector
(FID). This study conducted a comparison between FID and MS concentration
results, following the MS method described in SOP11.
Methods
In May 2020, five test e-liquids in replicates (A-E) were prepared at the
Istituto di Ricerche Farmacologiche Mario Negri and sent, with SOP 11, validation
document and results datasheet to 32 different laboratories all over the world
from WHO TobLabNet and EU-JATC (18 from JATC, ten from WHO TobLabNet
and four academic laboratories). Among thirty-two independent laboratories that
participated in the study, results were received from 30 laboratories.
Results
The e-liquids analyses, using the two approaches, were compared. Of the
30 laboratories surveyed, 21 utilized the FID approach exclusively, 7 opted for
MS detection, and 2 employed both methods. The findings demonstrated that
the gas chromatography-mass spectrometry (GC-MS) method offers comparable
analytical capabilities regarding accuracy and precision for nicotine, glycerol,
and propylene glycol to the FID approach. Through Pearson’s correlation test
with r≃1 showing a positive correlation between GC-FID and GC-MS data, and
the Student’s t-test, no significant differences between the two approaches were
revealed, showing p>0.005 for almost all three analytes in all samples.
Conclusions
This study indicates that it is possible to apply the available EU-JATC
SOP and the WHO TobLabNet SOP11 even in laboratories that do not have
access to an FID, for example, to analyze flavors, trace compounds or carcinogenic,
mutagenic, or toxic for reproduction (CMR) in electronic cigarette liquids. |
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| ISSN: | 1617-9625 |