Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.

Protein-energy malnutrition (PEM) is a risk factor for developing visceral leishmaniasis (VL). While nutrient deficiency can impair immunity, its mechanistic impact on protective adaptive immune responses following Leishmania infection remains unknown. To determine the potential negative impacts of...

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Main Authors: Laís Amorim Sacramento, Claudia Gonzalez-Lombana, Phillip Scott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-11-01
Series:PLoS Pathogens
Online Access:https://doi.org/10.1371/journal.ppat.1012716
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author Laís Amorim Sacramento
Claudia Gonzalez-Lombana
Phillip Scott
author_facet Laís Amorim Sacramento
Claudia Gonzalez-Lombana
Phillip Scott
author_sort Laís Amorim Sacramento
collection DOAJ
description Protein-energy malnutrition (PEM) is a risk factor for developing visceral leishmaniasis (VL). While nutrient deficiency can impair immunity, its mechanistic impact on protective adaptive immune responses following Leishmania infection remains unknown. To determine the potential negative impacts of malnutrition on anti-parasitic responses in chronic VL, we provided mice with a polynutrient-deficient diet (deficient protein, energy, zinc, and iron) that mimics moderate human malnutrition. The polynutrient-deficient diet resulted in growth stunting and reduced mass of visceral organs and following infection with Leishmania infantum, malnourished-mice harbored more parasites in the spleen and liver. Malnourished and infected mice also had fewer T lymphocytes, with reduced T cell production of IFN-γ required for parasite clearance and enhanced production of the immunosuppressive cytokine, IL-10. To determine if IL-10 was causative in disease progression in the malnourished mice, we treated infected mice with monoclonal antibody α-IL-10R. α-IL-10R treatment reduced the parasite number in malnourished mice, restored the number of T cells producing IFN-γ, and enhanced hepatic granuloma formation. Our results indicate that malnutrition increases VL susceptibility due to defective IFN-γ-mediated immunity attributable to increased IL-10 production.
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spelling doaj-art-6ed50ec7d7ae4c4a82732fb0ce16ad572024-11-28T05:31:03ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742024-11-012011e101271610.1371/journal.ppat.1012716Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.Laís Amorim SacramentoClaudia Gonzalez-LombanaPhillip ScottProtein-energy malnutrition (PEM) is a risk factor for developing visceral leishmaniasis (VL). While nutrient deficiency can impair immunity, its mechanistic impact on protective adaptive immune responses following Leishmania infection remains unknown. To determine the potential negative impacts of malnutrition on anti-parasitic responses in chronic VL, we provided mice with a polynutrient-deficient diet (deficient protein, energy, zinc, and iron) that mimics moderate human malnutrition. The polynutrient-deficient diet resulted in growth stunting and reduced mass of visceral organs and following infection with Leishmania infantum, malnourished-mice harbored more parasites in the spleen and liver. Malnourished and infected mice also had fewer T lymphocytes, with reduced T cell production of IFN-γ required for parasite clearance and enhanced production of the immunosuppressive cytokine, IL-10. To determine if IL-10 was causative in disease progression in the malnourished mice, we treated infected mice with monoclonal antibody α-IL-10R. α-IL-10R treatment reduced the parasite number in malnourished mice, restored the number of T cells producing IFN-γ, and enhanced hepatic granuloma formation. Our results indicate that malnutrition increases VL susceptibility due to defective IFN-γ-mediated immunity attributable to increased IL-10 production.https://doi.org/10.1371/journal.ppat.1012716
spellingShingle Laís Amorim Sacramento
Claudia Gonzalez-Lombana
Phillip Scott
Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
PLoS Pathogens
title Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
title_full Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
title_fullStr Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
title_full_unstemmed Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
title_short Malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing IL-10 production.
title_sort malnutrition disrupts adaptive immunity during visceral leishmaniasis by enhancing il 10 production
url https://doi.org/10.1371/journal.ppat.1012716
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AT phillipscott malnutritiondisruptsadaptiveimmunityduringvisceralleishmaniasisbyenhancingil10production