Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging
Near-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2003-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200303121 |
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| _version_ | 1841561857744175104 |
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| author | Yuhui Lin Ralph Weissleder Ching-Hsuan Tung |
| author_facet | Yuhui Lin Ralph Weissleder Ching-Hsuan Tung |
| author_sort | Yuhui Lin |
| collection | DOAJ |
| description | Near-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the synthesis of four new nonsymmetrical sulfhydryl-reactive cyanine NIRF with excellent optical and chemical properties. Each fluorochrome was designed to contain an iodoacetamido group that reacts specifically with sulfhydryl-containing molecules. The synthesized fluorochromes were used to label model peptides and sulfhydryl-containing biomolecules. |
| format | Article |
| id | doaj-art-6ecce7f63d1c4b10b3e55acc222d5557 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2003-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-6ecce7f63d1c4b10b3e55acc222d55572025-01-03T01:23:07ZengSAGE PublishingMolecular Imaging1536-01212003-04-01210.1162/1535350020030312110.1162_15353500200303121Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence ImagingYuhui LinRalph WeisslederChing-Hsuan TungNear-infrared fluorochromes (NIRF) are useful compounds for diverse biotechnology applications and for in vivo biomedical imaging. Such NIRF must have high quantum yield, be biocompatible, and be conjugatable to a wide variety of proteins, peptides, and other affinity ligands. Here, we describe the synthesis of four new nonsymmetrical sulfhydryl-reactive cyanine NIRF with excellent optical and chemical properties. Each fluorochrome was designed to contain an iodoacetamido group that reacts specifically with sulfhydryl-containing molecules. The synthesized fluorochromes were used to label model peptides and sulfhydryl-containing biomolecules.https://doi.org/10.1162/15353500200303121 |
| spellingShingle | Yuhui Lin Ralph Weissleder Ching-Hsuan Tung Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging Molecular Imaging |
| title | Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
| title_full | Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
| title_fullStr | Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
| title_full_unstemmed | Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
| title_short | Synthesis and Properties of Sulfhydryl-Reactive Near-Infrared Cyanine Fluorochromes for Fluorescence Imaging |
| title_sort | synthesis and properties of sulfhydryl reactive near infrared cyanine fluorochromes for fluorescence imaging |
| url | https://doi.org/10.1162/15353500200303121 |
| work_keys_str_mv | AT yuhuilin synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging AT ralphweissleder synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging AT chinghsuantung synthesisandpropertiesofsulfhydrylreactivenearinfraredcyaninefluorochromesforfluorescenceimaging |