The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification
The cryopreservation of <i>Anthurium andraeanum</i> germplasm resources is extremely important for the production and selection of new varieties. At present, the cryopreservation procedure for the callus of <i>A. andraeanum</i> has not been established. In this study, the lea...
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2024-11-01
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| author | Yiying Zhang Shan Deng Huifeng Lin Yunxia Chu Jingyan Huang Shouguo Li Fazhuang Lin Sumei Zhang Weilan Jiang Li Ren Hairong Chen |
| author_facet | Yiying Zhang Shan Deng Huifeng Lin Yunxia Chu Jingyan Huang Shouguo Li Fazhuang Lin Sumei Zhang Weilan Jiang Li Ren Hairong Chen |
| author_sort | Yiying Zhang |
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| description | The cryopreservation of <i>Anthurium andraeanum</i> germplasm resources is extremely important for the production and selection of new varieties. At present, the cryopreservation procedure for the callus of <i>A. andraeanum</i> has not been established. In this study, the leaves of <i>A. andraeanum</i> were used as explants to culture the callus. The cryopreservation procedure of the callus by vitrification was initially established by using the orthogonal experimental method of four factors and three levels in the preculture, loading, and dehydration steps. Furthermore, the vitrification-based cryopreservation was optimized by changing the preculture temperature and loading solution and adding exogenous substances to the plant vitrification solution (PVS2). In this procedure, the callus was precultured at 25 °C for 2 d, and loaded in 50% PVS2 at 25 °C for 60 min. The callus was dehydrated with PVS2 containing 0.08 mM reduced glutathione (GSH) at 0 °C for 60 min. After rapid-cooling in liquid nitrogen for 1 h, it was rapid-warming in a water bath at 40 °C for 90 s and unloaded for 30 min. After 1 d of recovery, the cell relative survival rate of the cryopreserved callus was 64.60%. The results provide a valuable basic and effective method for the long-term conservation of <i>A. andraeanum</i> germplasm resources. |
| format | Article |
| id | doaj-art-6ecab4ea11e74f6d92d537b95f6ea956 |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-11-01 |
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| series | Plants |
| spelling | doaj-art-6ecab4ea11e74f6d92d537b95f6ea9562024-11-08T14:39:39ZengMDPI AGPlants2223-77472024-11-011321310610.3390/plants13213106The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by VitrificationYiying Zhang0Shan Deng1Huifeng Lin2Yunxia Chu3Jingyan Huang4Shouguo Li5Fazhuang Lin6Sumei Zhang7Weilan Jiang8Li Ren9Hairong Chen10Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaFlower Research Institute, Sanming Academy of Agricultural Scienses, No. 2, Str. Qiujiang Zhuyuan, Shaxian, Sanming 365051, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaFlower Research Institute, Sanming Academy of Agricultural Scienses, No. 2, Str. Qiujiang Zhuyuan, Shaxian, Sanming 365051, ChinaCollege of Bioscience and Biotechnology, Yangzhou University, No. 48, Rd. Wenhui, Yangzhou 225009, ChinaSchool of Agriculture, Anshun University, No. 25, Rd. Xueyuan, Anshun 561000, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaInstitute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, No. 888, Rd. Yezhuang, Shanghai 201403, ChinaThe cryopreservation of <i>Anthurium andraeanum</i> germplasm resources is extremely important for the production and selection of new varieties. At present, the cryopreservation procedure for the callus of <i>A. andraeanum</i> has not been established. In this study, the leaves of <i>A. andraeanum</i> were used as explants to culture the callus. The cryopreservation procedure of the callus by vitrification was initially established by using the orthogonal experimental method of four factors and three levels in the preculture, loading, and dehydration steps. Furthermore, the vitrification-based cryopreservation was optimized by changing the preculture temperature and loading solution and adding exogenous substances to the plant vitrification solution (PVS2). In this procedure, the callus was precultured at 25 °C for 2 d, and loaded in 50% PVS2 at 25 °C for 60 min. The callus was dehydrated with PVS2 containing 0.08 mM reduced glutathione (GSH) at 0 °C for 60 min. After rapid-cooling in liquid nitrogen for 1 h, it was rapid-warming in a water bath at 40 °C for 90 s and unloaded for 30 min. After 1 d of recovery, the cell relative survival rate of the cryopreserved callus was 64.60%. The results provide a valuable basic and effective method for the long-term conservation of <i>A. andraeanum</i> germplasm resources.https://www.mdpi.com/2223-7747/13/21/3106Anthurium andraeanumcallus culturecryopreservationvitrification |
| spellingShingle | Yiying Zhang Shan Deng Huifeng Lin Yunxia Chu Jingyan Huang Shouguo Li Fazhuang Lin Sumei Zhang Weilan Jiang Li Ren Hairong Chen The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification Plants Anthurium andraeanum callus culture cryopreservation vitrification |
| title | The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification |
| title_full | The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification |
| title_fullStr | The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification |
| title_full_unstemmed | The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification |
| title_short | The Development of a Procedure for the Cryopreservation of the Callus of <i>Anthurium andraeanum</i> by Vitrification |
| title_sort | development of a procedure for the cryopreservation of the callus of i anthurium andraeanum i by vitrification |
| topic | Anthurium andraeanum callus culture cryopreservation vitrification |
| url | https://www.mdpi.com/2223-7747/13/21/3106 |
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