Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp
In this study, an eight-week culture trial of <i>Penaeus vannamei</i> juveniles was conducted in commercial intensive systems to compare the impacts of biochar and molasses addition on biofloc nitrifying bacteria and inorganic nitrogen dynamics under limited water exchange conditions. Du...
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2024-12-01
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| author | Wujie Xu Demin Zhang Haochang Su Yu Xu Xiaojuan Hu Guoliang Wen Yucheng Cao |
| author_facet | Wujie Xu Demin Zhang Haochang Su Yu Xu Xiaojuan Hu Guoliang Wen Yucheng Cao |
| author_sort | Wujie Xu |
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| description | In this study, an eight-week culture trial of <i>Penaeus vannamei</i> juveniles was conducted in commercial intensive systems to compare the impacts of biochar and molasses addition on biofloc nitrifying bacteria and inorganic nitrogen dynamics under limited water exchange conditions. During the trial, the biofloc concentration (in terms of VSS and TSS), quantities of total bacteria (TB) and total <i>Vibrio</i> (TV), and ratio of TV/TB in the culture water were lower in the biochar group compared to the molasses group. Metagenomic sequencing analysis revealed that the bacterial community composition of bioflocs showed higher α-diversity and complexity in the biochar group compared to the molasses group. Moreover, the abundance of nitrifying bacterial genera and functional genes in bioflocs was higher in the biochar group compared to the molasses group. Inorganic nitrogen dynamics showed that NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N were better controlled in the biochar group compared to the molasses group, as reflected by lower peaks of NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N and higher NO<sub>3</sub><sup>−</sup>-N concentrations. Excellent production performance of shrimp was achieved, which in turn proved the reliable effect of biochar addition on the mediation of inorganic nitrogen transformation through nitrifying bacteria. These results showed that biochar addition could promote biofloc nitrifying bacteria and nitrification to more effectively control harmful nitrogen for shrimp efficient production. This study provides a practical example for the biochar application in biofloc-based systems for intensive aquaculture. |
| format | Article |
| id | doaj-art-6e9c094f52b94ae4b6c20ac6bee670c0 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-6e9c094f52b94ae4b6c20ac6bee670c02024-12-27T14:41:37ZengMDPI AGMicroorganisms2076-26072024-12-011212258110.3390/microorganisms12122581Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of ShrimpWujie Xu0Demin Zhang1Haochang Su2Yu Xu3Xiaojuan Hu4Guoliang Wen5Yucheng Cao6South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ningbo University, Ningbo 315000, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, ChinaIn this study, an eight-week culture trial of <i>Penaeus vannamei</i> juveniles was conducted in commercial intensive systems to compare the impacts of biochar and molasses addition on biofloc nitrifying bacteria and inorganic nitrogen dynamics under limited water exchange conditions. During the trial, the biofloc concentration (in terms of VSS and TSS), quantities of total bacteria (TB) and total <i>Vibrio</i> (TV), and ratio of TV/TB in the culture water were lower in the biochar group compared to the molasses group. Metagenomic sequencing analysis revealed that the bacterial community composition of bioflocs showed higher α-diversity and complexity in the biochar group compared to the molasses group. Moreover, the abundance of nitrifying bacterial genera and functional genes in bioflocs was higher in the biochar group compared to the molasses group. Inorganic nitrogen dynamics showed that NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N were better controlled in the biochar group compared to the molasses group, as reflected by lower peaks of NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N and higher NO<sub>3</sub><sup>−</sup>-N concentrations. Excellent production performance of shrimp was achieved, which in turn proved the reliable effect of biochar addition on the mediation of inorganic nitrogen transformation through nitrifying bacteria. These results showed that biochar addition could promote biofloc nitrifying bacteria and nitrification to more effectively control harmful nitrogen for shrimp efficient production. This study provides a practical example for the biochar application in biofloc-based systems for intensive aquaculture.https://www.mdpi.com/2076-2607/12/12/2581shrimpbiocharbioflocbacterial communitynitrifying bacterianitrifying gene |
| spellingShingle | Wujie Xu Demin Zhang Haochang Su Yu Xu Xiaojuan Hu Guoliang Wen Yucheng Cao Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp Microorganisms shrimp biochar biofloc bacterial community nitrifying bacteria nitrifying gene |
| title | Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp |
| title_full | Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp |
| title_fullStr | Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp |
| title_full_unstemmed | Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp |
| title_short | Impact of Biochar Addition on Biofloc Nitrifying Bacteria and Inorganic Nitrogen Dynamics in an Intensive Aquaculture System of Shrimp |
| title_sort | impact of biochar addition on biofloc nitrifying bacteria and inorganic nitrogen dynamics in an intensive aquaculture system of shrimp |
| topic | shrimp biochar biofloc bacterial community nitrifying bacteria nitrifying gene |
| url | https://www.mdpi.com/2076-2607/12/12/2581 |
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