Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes
The present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from <i>Sargassum horneri</i>, a brown seaweed containing fucosterol (6.22 ± 0.06 mg/g), sulfoquinovosyl glycerolipids (C<sub>23</sub>H<sub>43</sub>O<sub...
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MDPI AG
2024-11-01
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| Series: | Antioxidants |
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| author | Kirinde Gedara Isuru Sandanuwan Kirindage Arachchige Maheshika Kumari Jayasinghe Chang-Ik Ko Yong-Seok Ahn Soo-Jin Heo Eun-A Kim Nam-Ki Cho Ginnae Ahn |
| author_facet | Kirinde Gedara Isuru Sandanuwan Kirindage Arachchige Maheshika Kumari Jayasinghe Chang-Ik Ko Yong-Seok Ahn Soo-Jin Heo Eun-A Kim Nam-Ki Cho Ginnae Ahn |
| author_sort | Kirinde Gedara Isuru Sandanuwan Kirindage |
| collection | DOAJ |
| description | The present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from <i>Sargassum horneri</i>, a brown seaweed containing fucosterol (6.22 ± 0.06 mg/g), sulfoquinovosyl glycerolipids (C<sub>23</sub>H<sub>43</sub>O<sub>11</sub>S, C<sub>25</sub>H<sub>45</sub>O<sub>11</sub>S, C<sub>25</sub>H<sub>47</sub>O<sub>11</sub>S, C<sub>27</sub>H<sub>49</sub>O<sub>11</sub>S), and polyphenols, against oxidative damage in ultraviolet B (UVB)-exposed HaCaT keratinocytes. USHE indicated antioxidant activity in ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. After screening experiments, 15.6, 31.3, and 62.5 µg/mL concentrations of USHE and ascorbic acid as positive control were selected to be used throughout the investigation. USHE increased cell viability by markedly reducing the production of intracellular reactive oxygen species (ROS) in UVB-exposed HaCaT keratinocytes. Additionally, USHE reduced the apoptosis and sub-G<sub>1</sub> cell population and increased the mitochondrial membrane potential. Moreover, USHE modulated the protein expression levels of anti-apoptotic molecules (Bcl-xL, Bcl-2, and PARP) and pro-apoptotic molecules (Bax, cleaved caspase-3, p53, cleaved PARP, and cytochrome C). This modulation accorded with the upregulation of cytosolic heme oxygenase (HO)-1, NAD(P)H quinone oxidoreductase 1 (NQO 1), and nuclear factor erythroid-2-related factor 2 (Nrf2), collectively known as components of the antioxidant system. These findings suggest that USHE has a photoprotective effect on UVB-exposed HaCaT keratinocytes and can be utilized to develop cosmeceuticals for UVB protection. |
| format | Article |
| id | doaj-art-6e88df0ad1f848149765a60dfd4fce6e |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-6e88df0ad1f848149765a60dfd4fce6e2024-11-26T17:47:02ZengMDPI AGAntioxidants2076-39212024-11-011311134210.3390/antiox13111342Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT KeratinocytesKirinde Gedara Isuru Sandanuwan Kirindage0Arachchige Maheshika Kumari Jayasinghe1Chang-Ik Ko2Yong-Seok Ahn3Soo-Jin Heo4Eun-A Kim5Nam-Ki Cho6Ginnae Ahn7Department of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of KoreaDepartment of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of KoreaChoung Ryong Fisheries Co., Ltd., Jeju-si 63612, Republic of KoreaChoung Ryong Fisheries Co., Ltd., Jeju-si 63612, Republic of KoreaJeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of KoreaJeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju-si 63349, Republic of KoreaCollege of Pharmacy, Chonnam National University, Gwangju 61186, Republic of KoreaDepartment of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, Republic of KoreaThe present study investigated the photoprotective effect of the ultrasonic-assisted ethanol extract (USHE) from <i>Sargassum horneri</i>, a brown seaweed containing fucosterol (6.22 ± 0.06 mg/g), sulfoquinovosyl glycerolipids (C<sub>23</sub>H<sub>43</sub>O<sub>11</sub>S, C<sub>25</sub>H<sub>45</sub>O<sub>11</sub>S, C<sub>25</sub>H<sub>47</sub>O<sub>11</sub>S, C<sub>27</sub>H<sub>49</sub>O<sub>11</sub>S), and polyphenols, against oxidative damage in ultraviolet B (UVB)-exposed HaCaT keratinocytes. USHE indicated antioxidant activity in ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. After screening experiments, 15.6, 31.3, and 62.5 µg/mL concentrations of USHE and ascorbic acid as positive control were selected to be used throughout the investigation. USHE increased cell viability by markedly reducing the production of intracellular reactive oxygen species (ROS) in UVB-exposed HaCaT keratinocytes. Additionally, USHE reduced the apoptosis and sub-G<sub>1</sub> cell population and increased the mitochondrial membrane potential. Moreover, USHE modulated the protein expression levels of anti-apoptotic molecules (Bcl-xL, Bcl-2, and PARP) and pro-apoptotic molecules (Bax, cleaved caspase-3, p53, cleaved PARP, and cytochrome C). This modulation accorded with the upregulation of cytosolic heme oxygenase (HO)-1, NAD(P)H quinone oxidoreductase 1 (NQO 1), and nuclear factor erythroid-2-related factor 2 (Nrf2), collectively known as components of the antioxidant system. These findings suggest that USHE has a photoprotective effect on UVB-exposed HaCaT keratinocytes and can be utilized to develop cosmeceuticals for UVB protection.https://www.mdpi.com/2076-3921/13/11/1342<i>Sargassum horneri</i>photoprotective effectapoptosisoxidative stressantioxidant capacityultrasonic extraction |
| spellingShingle | Kirinde Gedara Isuru Sandanuwan Kirindage Arachchige Maheshika Kumari Jayasinghe Chang-Ik Ko Yong-Seok Ahn Soo-Jin Heo Eun-A Kim Nam-Ki Cho Ginnae Ahn Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes Antioxidants <i>Sargassum horneri</i> photoprotective effect apoptosis oxidative stress antioxidant capacity ultrasonic extraction |
| title | Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes |
| title_full | Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes |
| title_fullStr | Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes |
| title_full_unstemmed | Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes |
| title_short | Photoprotective Effect of Ultrasonic-Assisted Ethanol Extract from <i>Sargassum horneri</i> on UVB-Exposed HaCaT Keratinocytes |
| title_sort | photoprotective effect of ultrasonic assisted ethanol extract from i sargassum horneri i on uvb exposed hacat keratinocytes |
| topic | <i>Sargassum horneri</i> photoprotective effect apoptosis oxidative stress antioxidant capacity ultrasonic extraction |
| url | https://www.mdpi.com/2076-3921/13/11/1342 |
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