A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita
Besnoitia spp. are cyst-forming coccidian parasites with a broad host range, infecting various wild and domestic animal species. Northamerican opossums (Didelphis virginiana) are severely affected by the infection with B. darlingi. This study presents a case of infection with Besnoitia in a road-kil...
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Elsevier
2024-12-01
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| Series: | International Journal for Parasitology: Parasites and Wildlife |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213224424000944 |
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| author | Juan Pablo Arrabal Gastón Moré María Marcela Orozco Elisa Helman Juliana Notarnicola Walter Basso Bárbara Betina Hartmann Andrea Schapira Leonardo Minatel |
| author_facet | Juan Pablo Arrabal Gastón Moré María Marcela Orozco Elisa Helman Juliana Notarnicola Walter Basso Bárbara Betina Hartmann Andrea Schapira Leonardo Minatel |
| author_sort | Juan Pablo Arrabal |
| collection | DOAJ |
| description | Besnoitia spp. are cyst-forming coccidian parasites with a broad host range, infecting various wild and domestic animal species. Northamerican opossums (Didelphis virginiana) are severely affected by the infection with B. darlingi. This study presents a case of infection with Besnoitia in a road-killed female southern black-eared opossum (Didelphis aurita) in Misiones, Argentina. Many 0.5–1 mm cysts were observed in several muscles and visceral organs and were microscopically identified in skeletal muscles, tongue, and heart. Histological analysis disclosed multiple spherical cysts with a myriad of bradyzoites like-cells and a well-defined cyst wall. A small number of degenerate and ruptured cysts, surrounded by mild to moderate inflammation were observed. Genomic DNA from an individual cyst and muscle was extracted and ITS1 marker and 18S rRNA gene fragments from sarcocystid protozoa were successfully amplified by PCR and sequenced. The 18S sequence exhibited 100% identity with sequences of B. darlingi and B. oryctofelisi. Comparison of the complete ITS1 sequence (259 bp) revealed an identity of 99.2% with B. oryctofelisi and 97.7% with B. darlingi. This result together with the phylogeny positioning, suggest that the Besnoitia sp. in the present case differ from B. darlingi, being closely related with B. oryctofelisi. |
| format | Article |
| id | doaj-art-6e6fb088cf9c4b178f9729d93d7eb4c7 |
| institution | Kabale University |
| issn | 2213-2244 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal for Parasitology: Parasites and Wildlife |
| spelling | doaj-art-6e6fb088cf9c4b178f9729d93d7eb4c72024-12-12T05:21:28ZengElsevierInternational Journal for Parasitology: Parasites and Wildlife2213-22442024-12-0125100998A putative new Besnoitia species in the southern black-eared opossum Didelphis auritaJuan Pablo Arrabal0Gastón Moré1María Marcela Orozco2Elisa Helman3Juliana Notarnicola4Walter Basso5Bárbara Betina Hartmann6Andrea Schapira7Leonardo Minatel8Instituto de Biología Subtropical IBS-CONICET, Universidad Nacional de Misiones- UNaM, Av. Tres Fronteras 183, Puerto Iguazú, CP 3370, Misiones, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina; Laboratorio de Inmunoparasitología (LAINPA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, (B1904), La Plata, Buenos Aires, Argentina; Institute of Parasitology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, Bern, 3012, Switzerland; Corresponding author. Institute of Parasitology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, Bern, 3012, Switzerland.Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina; Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Av. Int. Guiraldes, (C1428EGA), Ciudad Autónoma de Buenos Aires, ArgentinaConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina; Laboratorio de Inmunoparasitología (LAINPA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, Calle 60 y 118, (B1904), La Plata, Buenos Aires, Argentina; Grupo de Bioestadística Aplicada (GBA), Instituto de Cálculo Numérico Rebeca Cherep de Guber, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (FCEyN-UBA), Intendente Güiraldes 2160, Ciudad Universitaria, Edificio 0 + Infinito (C1428EGA), Buenos Aires, ArgentinaInstituto de Biología Subtropical IBS-CONICET, Universidad Nacional de Misiones- UNaM, Av. Tres Fronteras 183, Puerto Iguazú, CP 3370, Misiones, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, ArgentinaInstitute of Parasitology, Vetsuisse Faculty, University of Bern, Länggassstrasse 122, Bern, 3012, SwitzerlandInstituto de Biología Subtropical IBS-CONICET, Universidad Nacional de Misiones- UNaM, Av. Tres Fronteras 183, Puerto Iguazú, CP 3370, Misiones, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Ciudad Autónoma de Buenos Aires, Buenos Aires, ArgentinaUniversidad de Buenos Aires, Facultad de Ciencias Veterinarias, Chorroarín 280, (C1427CWO), Ciudad Autónoma de Buenos Aires, ArgentinaUniversidad de Buenos Aires, Facultad de Ciencias Veterinarias, Chorroarín 280, (C1427CWO), Ciudad Autónoma de Buenos Aires, ArgentinaBesnoitia spp. are cyst-forming coccidian parasites with a broad host range, infecting various wild and domestic animal species. Northamerican opossums (Didelphis virginiana) are severely affected by the infection with B. darlingi. This study presents a case of infection with Besnoitia in a road-killed female southern black-eared opossum (Didelphis aurita) in Misiones, Argentina. Many 0.5–1 mm cysts were observed in several muscles and visceral organs and were microscopically identified in skeletal muscles, tongue, and heart. Histological analysis disclosed multiple spherical cysts with a myriad of bradyzoites like-cells and a well-defined cyst wall. A small number of degenerate and ruptured cysts, surrounded by mild to moderate inflammation were observed. Genomic DNA from an individual cyst and muscle was extracted and ITS1 marker and 18S rRNA gene fragments from sarcocystid protozoa were successfully amplified by PCR and sequenced. The 18S sequence exhibited 100% identity with sequences of B. darlingi and B. oryctofelisi. Comparison of the complete ITS1 sequence (259 bp) revealed an identity of 99.2% with B. oryctofelisi and 97.7% with B. darlingi. This result together with the phylogeny positioning, suggest that the Besnoitia sp. in the present case differ from B. darlingi, being closely related with B. oryctofelisi.http://www.sciencedirect.com/science/article/pii/S2213224424000944Besnoitia sp.Didelphis auritaArgentinaInfectionPCRHistopathology |
| spellingShingle | Juan Pablo Arrabal Gastón Moré María Marcela Orozco Elisa Helman Juliana Notarnicola Walter Basso Bárbara Betina Hartmann Andrea Schapira Leonardo Minatel A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita International Journal for Parasitology: Parasites and Wildlife Besnoitia sp. Didelphis aurita Argentina Infection PCR Histopathology |
| title | A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita |
| title_full | A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita |
| title_fullStr | A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita |
| title_full_unstemmed | A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita |
| title_short | A putative new Besnoitia species in the southern black-eared opossum Didelphis aurita |
| title_sort | putative new besnoitia species in the southern black eared opossum didelphis aurita |
| topic | Besnoitia sp. Didelphis aurita Argentina Infection PCR Histopathology |
| url | http://www.sciencedirect.com/science/article/pii/S2213224424000944 |
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