Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis

Medulloblastoma, a malignant pediatric brain tumor, has a poor prognosis upon relapse, highlighting a critical clinical need. Our previous research linked medulloblastoma cell radioresistance to integrin-αvβ3 expression. β3-depleted (β3_KO) medulloblastoma cells exhibit lipid hydroxyperoxide accumul...

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Main Authors: Célia Gotorbe, Fabien Segui, William Echavidre, Jérôme Durivault, Thays Blanchard, Valérie Vial, Marina Pagnuzzi-Boncompagni, Rémy Villeneuve, Régis Amblard, Nicolas Garnier, Cécile Ortholan, Benjamin Serrano, Vincent Picco, Jacques Pouysségur, Milica Vucetic, Christopher Montemagno
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Current Oncology
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Online Access:https://www.mdpi.com/1718-7729/31/11/545
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author Célia Gotorbe
Fabien Segui
William Echavidre
Jérôme Durivault
Thays Blanchard
Valérie Vial
Marina Pagnuzzi-Boncompagni
Rémy Villeneuve
Régis Amblard
Nicolas Garnier
Cécile Ortholan
Benjamin Serrano
Vincent Picco
Jacques Pouysségur
Milica Vucetic
Christopher Montemagno
author_facet Célia Gotorbe
Fabien Segui
William Echavidre
Jérôme Durivault
Thays Blanchard
Valérie Vial
Marina Pagnuzzi-Boncompagni
Rémy Villeneuve
Régis Amblard
Nicolas Garnier
Cécile Ortholan
Benjamin Serrano
Vincent Picco
Jacques Pouysségur
Milica Vucetic
Christopher Montemagno
author_sort Célia Gotorbe
collection DOAJ
description Medulloblastoma, a malignant pediatric brain tumor, has a poor prognosis upon relapse, highlighting a critical clinical need. Our previous research linked medulloblastoma cell radioresistance to integrin-αvβ3 expression. β3-depleted (β3_KO) medulloblastoma cells exhibit lipid hydroxyperoxide accumulation after radiotherapy, indicating ferroptosis, a regulated cell death induced by ROS and inhibited by antioxidants such as cysteine, glutathione (GSH), and glutathione peroxidase 4 (GPx4). However, the link between αvβ3 expression, ferroptosis inhibition, and sensitivity to radiotherapy remains unclear. We showed that irradiated β3_KO medulloblastoma cells primarily die by ferroptosis, with β3-subunit expression correlating with radiotherapy sensitivity and anti-ferroptotic protein levels. Our findings suggest that integrin-αvβ3 signaling boosts oxidative stress resilience via mTORC1. Thus, targeting integrin-αvβ3 could enhance radiotherapy efficacy in medulloblastoma by inducing ferroptotic cell death.
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language English
publishDate 2024-11-01
publisher MDPI AG
record_format Article
series Current Oncology
spelling doaj-art-6e6ee2efabe044e8922a5b5df1a8d7742024-11-26T17:59:06ZengMDPI AGCurrent Oncology1198-00521718-77292024-11-0131117390740210.3390/curroncol31110545Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via FerroptosisCélia Gotorbe0Fabien Segui1William Echavidre2Jérôme Durivault3Thays Blanchard4Valérie Vial5Marina Pagnuzzi-Boncompagni6Rémy Villeneuve7Régis Amblard8Nicolas Garnier9Cécile Ortholan10Benjamin Serrano11Vincent Picco12Jacques Pouysségur13Milica Vucetic14Christopher Montemagno15Biomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoRadiophysics Department, Princess Grace Hospital, 98000 Monaco, MonacoRadiophysics Department, Princess Grace Hospital, 98000 Monaco, MonacoRadiophysics Department, Princess Grace Hospital, 98000 Monaco, MonacoRadiotherapy Department, Princess Grace Hospital, 98000 Monaco, MonacoRadiophysics Department, Princess Grace Hospital, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoBiomedical Department, Centre Scientifique de Monaco, 98000 Monaco, MonacoMedulloblastoma, a malignant pediatric brain tumor, has a poor prognosis upon relapse, highlighting a critical clinical need. Our previous research linked medulloblastoma cell radioresistance to integrin-αvβ3 expression. β3-depleted (β3_KO) medulloblastoma cells exhibit lipid hydroxyperoxide accumulation after radiotherapy, indicating ferroptosis, a regulated cell death induced by ROS and inhibited by antioxidants such as cysteine, glutathione (GSH), and glutathione peroxidase 4 (GPx4). However, the link between αvβ3 expression, ferroptosis inhibition, and sensitivity to radiotherapy remains unclear. We showed that irradiated β3_KO medulloblastoma cells primarily die by ferroptosis, with β3-subunit expression correlating with radiotherapy sensitivity and anti-ferroptotic protein levels. Our findings suggest that integrin-αvβ3 signaling boosts oxidative stress resilience via mTORC1. Thus, targeting integrin-αvβ3 could enhance radiotherapy efficacy in medulloblastoma by inducing ferroptotic cell death.https://www.mdpi.com/1718-7729/31/11/545integrin-αvβ3medulloblastomacilengitideradiotherapyferroptosis
spellingShingle Célia Gotorbe
Fabien Segui
William Echavidre
Jérôme Durivault
Thays Blanchard
Valérie Vial
Marina Pagnuzzi-Boncompagni
Rémy Villeneuve
Régis Amblard
Nicolas Garnier
Cécile Ortholan
Benjamin Serrano
Vincent Picco
Jacques Pouysségur
Milica Vucetic
Christopher Montemagno
Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
Current Oncology
integrin-αvβ3
medulloblastoma
cilengitide
radiotherapy
ferroptosis
title Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
title_full Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
title_fullStr Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
title_full_unstemmed Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
title_short Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis
title_sort exploiting integrin αvβ3 to enhance radiotherapy efficacy in medulloblastoma via ferroptosis
topic integrin-αvβ3
medulloblastoma
cilengitide
radiotherapy
ferroptosis
url https://www.mdpi.com/1718-7729/31/11/545
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