Unveiling the therapeutic potential of octreotide in treating morphine dependence

Abstract The potential of octreotide (OCT), an octapeptide synthetic somatostatin agonist, to mitigate addiction, specifically morphine dependence, was evaluated in a comprehensive approach involving behavioral, molecular, histological, and docking studies. OCT decreased addiction-related behaviors...

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Main Authors: Mohammad Shabani, Zeynab Pirmoradi, Moazamehosadat Razavinasab, Amin Hassanshahi, Reyhaneh Naderi, Zahra Esmaili, Mohammad Navid Ebrahimi, Mehran Ilaghi, Mohammad Amin Langarizadeh, Amir Hossain Moqaddari, Leili Rouhi, Meysam Ahmadi-Zeidabadi, Maryam Amiri, Kristi A. Kohlmeier, Sajad Amiri, Hamid Forootanfar, Mohammad Banazadeh
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-12761-6
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author Mohammad Shabani
Zeynab Pirmoradi
Moazamehosadat Razavinasab
Amin Hassanshahi
Reyhaneh Naderi
Zahra Esmaili
Mohammad Navid Ebrahimi
Mehran Ilaghi
Mohammad Amin Langarizadeh
Amir Hossain Moqaddari
Leili Rouhi
Meysam Ahmadi-Zeidabadi
Maryam Amiri
Kristi A. Kohlmeier
Sajad Amiri
Hamid Forootanfar
Mohammad Banazadeh
author_facet Mohammad Shabani
Zeynab Pirmoradi
Moazamehosadat Razavinasab
Amin Hassanshahi
Reyhaneh Naderi
Zahra Esmaili
Mohammad Navid Ebrahimi
Mehran Ilaghi
Mohammad Amin Langarizadeh
Amir Hossain Moqaddari
Leili Rouhi
Meysam Ahmadi-Zeidabadi
Maryam Amiri
Kristi A. Kohlmeier
Sajad Amiri
Hamid Forootanfar
Mohammad Banazadeh
author_sort Mohammad Shabani
collection DOAJ
description Abstract The potential of octreotide (OCT), an octapeptide synthetic somatostatin agonist, to mitigate addiction, specifically morphine dependence, was evaluated in a comprehensive approach involving behavioral, molecular, histological, and docking studies. OCT decreased addiction-related behaviors in Wistar rats previously exposed to morphine, normalizing their exploratory activities and reducing drug-seeking behaviors. Histological assessments revealed octreotide-induced reductions in opioid-induced neuronal damage, suggesting a neuroprotective function. Octreotide’s behavioral and histopathological effects were associated with regulation of molecular pathways known to be critical in morphine dependence processes, including TLR4, BDNF, SIRT1, and mTOR. Supplementary docking studies elucidated octreotide’s high affinity for addiction-related targets, suggesting that a biological interaction between OCT and players in pathways known to play a role in addiction could be involved in behavioral and cellular effects noted. When our results are taken together, we conclude that OCT could be a promising candidate for opioid dependence treatment and underscore the necessity for further preclinical and clinical investigations.
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spelling doaj-art-6df6e683d1bc4cc2a3ad7adef2ddca252025-08-20T04:02:45ZengNature PortfolioScientific Reports2045-23222025-08-0115111810.1038/s41598-025-12761-6Unveiling the therapeutic potential of octreotide in treating morphine dependenceMohammad Shabani0Zeynab Pirmoradi1Moazamehosadat Razavinasab2Amin Hassanshahi3Reyhaneh Naderi4Zahra Esmaili5Mohammad Navid Ebrahimi6Mehran Ilaghi7Mohammad Amin Langarizadeh8Amir Hossain Moqaddari9Leili Rouhi10Meysam Ahmadi-Zeidabadi11Maryam Amiri12Kristi A. Kohlmeier13Sajad Amiri14Hamid Forootanfar15Mohammad Banazadeh16Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesDepartment of Physiology, School of Medicine, Bam University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesDepartment of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical SciencesMedicinal Plants and Drugs Research Institute, Shahid Beheshti UniversityDepartment of Psychology and Health Studies, University of SaskatchewanNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesNeuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical SciencesDepartment of Drug Design and Pharmacology, Faculty of Health Sciences, University of CopenhagenSchool of Medicine, Tehran University of Medical SciencesPharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical SciencesPharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical SciencesAbstract The potential of octreotide (OCT), an octapeptide synthetic somatostatin agonist, to mitigate addiction, specifically morphine dependence, was evaluated in a comprehensive approach involving behavioral, molecular, histological, and docking studies. OCT decreased addiction-related behaviors in Wistar rats previously exposed to morphine, normalizing their exploratory activities and reducing drug-seeking behaviors. Histological assessments revealed octreotide-induced reductions in opioid-induced neuronal damage, suggesting a neuroprotective function. Octreotide’s behavioral and histopathological effects were associated with regulation of molecular pathways known to be critical in morphine dependence processes, including TLR4, BDNF, SIRT1, and mTOR. Supplementary docking studies elucidated octreotide’s high affinity for addiction-related targets, suggesting that a biological interaction between OCT and players in pathways known to play a role in addiction could be involved in behavioral and cellular effects noted. When our results are taken together, we conclude that OCT could be a promising candidate for opioid dependence treatment and underscore the necessity for further preclinical and clinical investigations.https://doi.org/10.1038/s41598-025-12761-6OctreotideAddictionMolecular dockingRat
spellingShingle Mohammad Shabani
Zeynab Pirmoradi
Moazamehosadat Razavinasab
Amin Hassanshahi
Reyhaneh Naderi
Zahra Esmaili
Mohammad Navid Ebrahimi
Mehran Ilaghi
Mohammad Amin Langarizadeh
Amir Hossain Moqaddari
Leili Rouhi
Meysam Ahmadi-Zeidabadi
Maryam Amiri
Kristi A. Kohlmeier
Sajad Amiri
Hamid Forootanfar
Mohammad Banazadeh
Unveiling the therapeutic potential of octreotide in treating morphine dependence
Scientific Reports
Octreotide
Addiction
Molecular docking
Rat
title Unveiling the therapeutic potential of octreotide in treating morphine dependence
title_full Unveiling the therapeutic potential of octreotide in treating morphine dependence
title_fullStr Unveiling the therapeutic potential of octreotide in treating morphine dependence
title_full_unstemmed Unveiling the therapeutic potential of octreotide in treating morphine dependence
title_short Unveiling the therapeutic potential of octreotide in treating morphine dependence
title_sort unveiling the therapeutic potential of octreotide in treating morphine dependence
topic Octreotide
Addiction
Molecular docking
Rat
url https://doi.org/10.1038/s41598-025-12761-6
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