Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis
<b>Background:</b> The lack of local availability for drugs in the colon can be addressed by preparing a self-microemulsifying drug delivery system (SMEDDS) of curcumin (Cur) which is ultimately used for the treatment of inflammatory bowel disease (IBD). <b>Methods:</b> From...
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| author | Nabeela Ameer Muhammad Hanif Ghulam Abbas Muhammad Azeem Khalid Mahmood Dure Shahwar Ahmed Khames Essam Mohamed Eissa Baher Daihom |
| author_facet | Nabeela Ameer Muhammad Hanif Ghulam Abbas Muhammad Azeem Khalid Mahmood Dure Shahwar Ahmed Khames Essam Mohamed Eissa Baher Daihom |
| author_sort | Nabeela Ameer |
| collection | DOAJ |
| description | <b>Background:</b> The lack of local availability for drugs in the colon can be addressed by preparing a self-microemulsifying drug delivery system (SMEDDS) of curcumin (Cur) which is ultimately used for the treatment of inflammatory bowel disease (IBD). <b>Methods:</b> From preformulation studies, Lauroglycol FCC (oil), Tween 80 (surfactant), Transcutol HP (co-surfactant), and Avicel (solid carrier) were selected for the preparation of blank liquid and solid Cur-loaded SMEDDSs (S-Cur-SMEDDSs). <b>Results:</b> Z-average size (12.36 ± 0.04 nm), zeta potential (−14.7 ± 0.08 mV), and polydispersity index (PDI) (0.155 ± 0.036) showed a comparative droplet surface area and charge of both SMEDDSs. The physicochemical stability of Cur in S-Cur-SMEDDSs was confirmed via FTIR, DSC, TGA, and XRD analyses, while morphological analysis through SEM and atomic force microscopy (AFM) confirmed Cur loading into SMEDDSs with an increased surface roughness root mean square (RMS) of 11.433 ± 0.91 nm, greater than the blank SMEDDS. Acute toxicity studies with an organ weight ratio and % hemolysis of 15.65 ± 1.32% at a high concentration of 600 mM showed that S-Cur-SMEDDSs are safe at a medium dose (0.2–0.8 g/kg/day). The excellent in vitro antioxidant (68.54 ± 1.42%) and anti-inflammatory properties (56.47 ± 1.17%) of S-Cur-SMEDDS proved its therapeutic efficacy for IBD. Finally, S-Cur-SMEDDS significantly improved acetic acid-induced IBD in albino rats through a reduction in the disease activity index (DAI) and macroscopic ulcer score (MUS) from 4.15 ± 0.21 to 1.62 ± 0.12 at 15 mg/kg/day dose, as confirmed via histopathological assay. <b>Conclusions:</b> Based on the above findings, S-Cur-SMEDDS appears to be a stable, less toxic, and more efficacious alternative for Cur delivery with strong competence in treating IBD. |
| format | Article |
| id | doaj-art-6de2f86401a042da9ebb9c02da5fc0e2 |
| institution | Kabale University |
| issn | 1999-4923 |
| language | English |
| publishDate | 2024-10-01 |
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| spelling | doaj-art-6de2f86401a042da9ebb9c02da5fc0e22024-11-26T18:17:51ZengMDPI AGPharmaceutics1999-49232024-10-011611140610.3390/pharmaceutics16111406Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic AnalysisNabeela Ameer0Muhammad Hanif1Ghulam Abbas2Muhammad Azeem3Khalid Mahmood4Dure Shahwar5Ahmed Khames6Essam Mohamed Eissa7Baher Daihom8Department of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan 60800, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan 60800, PakistanDepartment of Pharmaceutics, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad 38000, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan 60800, PakistanInstitute of Chemical Sciences, Bahauddin Zakariya University, Multan 60800, PakistanDepartment of Pharmaceutics, Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan 60800, PakistanDepartment of Pharmaceutics and Industrial Pharmacy, College of Pharmacy, Taif University, Taif 21944, Saudi ArabiaDepartment of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmaceutics and Industrial Pharmacy, Cairo University, Cairo 11562, Egypt<b>Background:</b> The lack of local availability for drugs in the colon can be addressed by preparing a self-microemulsifying drug delivery system (SMEDDS) of curcumin (Cur) which is ultimately used for the treatment of inflammatory bowel disease (IBD). <b>Methods:</b> From preformulation studies, Lauroglycol FCC (oil), Tween 80 (surfactant), Transcutol HP (co-surfactant), and Avicel (solid carrier) were selected for the preparation of blank liquid and solid Cur-loaded SMEDDSs (S-Cur-SMEDDSs). <b>Results:</b> Z-average size (12.36 ± 0.04 nm), zeta potential (−14.7 ± 0.08 mV), and polydispersity index (PDI) (0.155 ± 0.036) showed a comparative droplet surface area and charge of both SMEDDSs. The physicochemical stability of Cur in S-Cur-SMEDDSs was confirmed via FTIR, DSC, TGA, and XRD analyses, while morphological analysis through SEM and atomic force microscopy (AFM) confirmed Cur loading into SMEDDSs with an increased surface roughness root mean square (RMS) of 11.433 ± 0.91 nm, greater than the blank SMEDDS. Acute toxicity studies with an organ weight ratio and % hemolysis of 15.65 ± 1.32% at a high concentration of 600 mM showed that S-Cur-SMEDDSs are safe at a medium dose (0.2–0.8 g/kg/day). The excellent in vitro antioxidant (68.54 ± 1.42%) and anti-inflammatory properties (56.47 ± 1.17%) of S-Cur-SMEDDS proved its therapeutic efficacy for IBD. Finally, S-Cur-SMEDDS significantly improved acetic acid-induced IBD in albino rats through a reduction in the disease activity index (DAI) and macroscopic ulcer score (MUS) from 4.15 ± 0.21 to 1.62 ± 0.12 at 15 mg/kg/day dose, as confirmed via histopathological assay. <b>Conclusions:</b> Based on the above findings, S-Cur-SMEDDS appears to be a stable, less toxic, and more efficacious alternative for Cur delivery with strong competence in treating IBD.https://www.mdpi.com/1999-4923/16/11/1406curcuminself-microemulsifying drug delivery systemcolonacute toxicityinflammatory bowel disease |
| spellingShingle | Nabeela Ameer Muhammad Hanif Ghulam Abbas Muhammad Azeem Khalid Mahmood Dure Shahwar Ahmed Khames Essam Mohamed Eissa Baher Daihom Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis Pharmaceutics curcumin self-microemulsifying drug delivery system colon acute toxicity inflammatory bowel disease |
| title | Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis |
| title_full | Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis |
| title_fullStr | Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis |
| title_full_unstemmed | Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis |
| title_short | Treatment of Inflammatory Bowel Disease by Using Curcumin-Containing Self-Microemulsifying Delivery System: Macroscopic and Microscopic Analysis |
| title_sort | treatment of inflammatory bowel disease by using curcumin containing self microemulsifying delivery system macroscopic and microscopic analysis |
| topic | curcumin self-microemulsifying drug delivery system colon acute toxicity inflammatory bowel disease |
| url | https://www.mdpi.com/1999-4923/16/11/1406 |
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