A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021)
Ixodes ricinus is a key vector of several bacterial pathogens, including Borrelia and Rickettsia species. To assess bacterial prevalence and diversity, we characterised the microbiome of ticks collected from a single UK woodland, comparing different woodland types, life stages (adult male, female, a...
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Elsevier
2025-07-01
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| Series: | Ticks and Tick-Borne Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1877959X25000706 |
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| author | Mia L. White Jack M. Crook Kayleigh M. Hansford Daniel P. Carter Michael J. Elmore Colin Johnston Sara Gandy Kuiama Lewandowski Alexander Vaux Karen L. Osman Richard Vipond Jolyon M. Medlock Edward J Feil Steven T. Pullan |
| author_facet | Mia L. White Jack M. Crook Kayleigh M. Hansford Daniel P. Carter Michael J. Elmore Colin Johnston Sara Gandy Kuiama Lewandowski Alexander Vaux Karen L. Osman Richard Vipond Jolyon M. Medlock Edward J Feil Steven T. Pullan |
| author_sort | Mia L. White |
| collection | DOAJ |
| description | Ixodes ricinus is a key vector of several bacterial pathogens, including Borrelia and Rickettsia species. To assess bacterial prevalence and diversity, we characterised the microbiome of ticks collected from a single UK woodland, comparing different woodland types, life stages (adult male, female, and nymph), and sampling years (spring 2019–2021). Unlike previous studies that target short regions of the 16S rRNA gene, we used Nanopore sequencing to generate full-length 16S reads, allowing for higher taxonomic resolution. Our results showed significant microbiome differences across life stages, but not by woodland type or year. Rickettsia was the most abundant taxon, with prevalence varying by life stage and year. Borrelia was present in all life stages but absent in 2021 samples. Anaplasma abundance varied significantly by year and life stage, peaking in nymphs from Pine woodland. Bartonella was found across all woodland types, with the highest abundance in nymphs from Oak-dominated areas. |
| format | Article |
| id | doaj-art-6dc64cfe49d84a2d80cbfd7ab55aa1c6 |
| institution | Kabale University |
| issn | 1877-9603 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ticks and Tick-Borne Diseases |
| spelling | doaj-art-6dc64cfe49d84a2d80cbfd7ab55aa1c62025-08-20T03:55:48ZengElsevierTicks and Tick-Borne Diseases1877-96032025-07-0116410250610.1016/j.ttbdis.2025.102506A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021)Mia L. White0Jack M. Crook1Kayleigh M. Hansford2Daniel P. Carter3Michael J. Elmore4Colin Johnston5Sara Gandy6Kuiama Lewandowski7Alexander Vaux8Karen L. Osman9Richard Vipond10Jolyon M. Medlock11Edward J Feil12Steven T. Pullan13Diagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UK; Corresponding author.Diagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKMedical Entomology and Zoonoses Ecology, UKHSA, Porton Down, Salisbury, SP4 0JG, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKMedical Entomology and Zoonoses Ecology, UKHSA, Porton Down, Salisbury, SP4 0JG, UKMedical Entomology and Zoonoses Ecology, UKHSA, Porton Down, Salisbury, SP4 0JG, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKMedical Entomology and Zoonoses Ecology, UKHSA, Porton Down, Salisbury, SP4 0JG, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKMedical Entomology and Zoonoses Ecology, UKHSA, Porton Down, Salisbury, SP4 0JG, UKThe Milner Centre for Evolution, Department for Biology and Biochemistry, University of Bath, Bath, BA2 7AZ, UKDiagnostics & Pathogen Characterisation, UKHSA, Porton Down, Salisbury, SP4 0JG, UKIxodes ricinus is a key vector of several bacterial pathogens, including Borrelia and Rickettsia species. To assess bacterial prevalence and diversity, we characterised the microbiome of ticks collected from a single UK woodland, comparing different woodland types, life stages (adult male, female, and nymph), and sampling years (spring 2019–2021). Unlike previous studies that target short regions of the 16S rRNA gene, we used Nanopore sequencing to generate full-length 16S reads, allowing for higher taxonomic resolution. Our results showed significant microbiome differences across life stages, but not by woodland type or year. Rickettsia was the most abundant taxon, with prevalence varying by life stage and year. Borrelia was present in all life stages but absent in 2021 samples. Anaplasma abundance varied significantly by year and life stage, peaking in nymphs from Pine woodland. Bartonella was found across all woodland types, with the highest abundance in nymphs from Oak-dominated areas.http://www.sciencedirect.com/science/article/pii/S1877959X25000706Microbiome, Ixodes ricinusOxford nanopore technologiesGenomicsTick borne disease16S sequencing |
| spellingShingle | Mia L. White Jack M. Crook Kayleigh M. Hansford Daniel P. Carter Michael J. Elmore Colin Johnston Sara Gandy Kuiama Lewandowski Alexander Vaux Karen L. Osman Richard Vipond Jolyon M. Medlock Edward J Feil Steven T. Pullan A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) Ticks and Tick-Borne Diseases Microbiome, Ixodes ricinus Oxford nanopore technologies Genomics Tick borne disease 16S sequencing |
| title | A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) |
| title_full | A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) |
| title_fullStr | A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) |
| title_full_unstemmed | A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) |
| title_short | A full-length 16S survey using Nanopore sequencing to uncover the bacterial microbiome in Ixodes ricinus ticks from a single UK woodland, collected across three springs (2019-2021) |
| title_sort | full length 16s survey using nanopore sequencing to uncover the bacterial microbiome in ixodes ricinus ticks from a single uk woodland collected across three springs 2019 2021 |
| topic | Microbiome, Ixodes ricinus Oxford nanopore technologies Genomics Tick borne disease 16S sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S1877959X25000706 |
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