The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis
Summary: Cartilage endplate (CEP) in the intervertebral disc (IVD) contributes to vertebral level asymmetrically in congenital scoliosis (CS). However, the cellular compositions of CEP and the subsequent alteration of cellular environment in its neighboring annulus fibrosus and nucleus pulposus rema...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225008697 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849387485723885568 |
|---|---|
| author | Junfeng Wang Haodong Li Dong Fu Yiming Zheng Chuang Qian Lin Li Maoxiang Qian Dahui Wang |
| author_facet | Junfeng Wang Haodong Li Dong Fu Yiming Zheng Chuang Qian Lin Li Maoxiang Qian Dahui Wang |
| author_sort | Junfeng Wang |
| collection | DOAJ |
| description | Summary: Cartilage endplate (CEP) in the intervertebral disc (IVD) contributes to vertebral level asymmetrically in congenital scoliosis (CS). However, the cellular compositions of CEP and the subsequent alteration of cellular environment in its neighboring annulus fibrosus and nucleus pulposus remain unknown during the progressive scoliosis. Herein, this study resolved the single-cell landscape of IVD in CS. Chondrocytes in CS demonstrated a different trajectory and were enriched in the cytoskeleton dependent cytokinesis pathways. H19, ECRG4, CCN1, and CCN2 were the specific markers for CS, and DBP may be the critical transcription factor (TF) for CS. Notochord and pericyte were the dominative cell types in the cell-cell communications, among which NCAM and SEMA5 signaling were the unique pathways for CS. Collectively, the aberrant differentiation trajectory of chondrocytes may explain the vertebral dysplasia in CS, and these critical gene markers, TFs, and pathways identified in this study may provide potential therapeutic targets for CS. |
| format | Article |
| id | doaj-art-6dc5f1c2088e47b2b30f6648a4beb2f0 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-6dc5f1c2088e47b2b30f6648a4beb2f02025-08-20T03:53:47ZengElsevieriScience2589-00422025-06-0128611260810.1016/j.isci.2025.112608The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosisJunfeng Wang0Haodong Li1Dong Fu2Yiming Zheng3Chuang Qian4Lin Li5Maoxiang Qian6Dahui Wang7Department of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. ChinaDepartment of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. ChinaDepartment of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. ChinaDepartment of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. ChinaDepartment of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. ChinaDepartment of Hematology and Oncology, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Fudan University, Shanghai 201102, P.R. ChinaDepartment of Hematology and Oncology, Children’s Hospital of Fudan University, and Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical Sciences, Fudan University, Shanghai 201102, P.R. China; Corresponding authorDepartment of Orthopedics, Children’s Hospital of Fudan University, Shanghai 201102, P.R. China; Corresponding authorSummary: Cartilage endplate (CEP) in the intervertebral disc (IVD) contributes to vertebral level asymmetrically in congenital scoliosis (CS). However, the cellular compositions of CEP and the subsequent alteration of cellular environment in its neighboring annulus fibrosus and nucleus pulposus remain unknown during the progressive scoliosis. Herein, this study resolved the single-cell landscape of IVD in CS. Chondrocytes in CS demonstrated a different trajectory and were enriched in the cytoskeleton dependent cytokinesis pathways. H19, ECRG4, CCN1, and CCN2 were the specific markers for CS, and DBP may be the critical transcription factor (TF) for CS. Notochord and pericyte were the dominative cell types in the cell-cell communications, among which NCAM and SEMA5 signaling were the unique pathways for CS. Collectively, the aberrant differentiation trajectory of chondrocytes may explain the vertebral dysplasia in CS, and these critical gene markers, TFs, and pathways identified in this study may provide potential therapeutic targets for CS.http://www.sciencedirect.com/science/article/pii/S2589004225008697pathophysiologytranscriptomics |
| spellingShingle | Junfeng Wang Haodong Li Dong Fu Yiming Zheng Chuang Qian Lin Li Maoxiang Qian Dahui Wang The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis iScience pathophysiology transcriptomics |
| title | The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| title_full | The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| title_fullStr | The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| title_full_unstemmed | The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| title_short | The single-cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| title_sort | single cell transcription reveals the aberrant differentiation trajectory of chondrocytes in the intervertebral disc for congenital scoliosis |
| topic | pathophysiology transcriptomics |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225008697 |
| work_keys_str_mv | AT junfengwang thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT haodongli thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT dongfu thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT yimingzheng thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT chuangqian thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT linli thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT maoxiangqian thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT dahuiwang thesinglecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT junfengwang singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT haodongli singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT dongfu singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT yimingzheng singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT chuangqian singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT linli singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT maoxiangqian singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis AT dahuiwang singlecelltranscriptionrevealstheaberrantdifferentiationtrajectoryofchondrocytesintheintervertebraldiscforcongenitalscoliosis |