Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk
IntroductionHuman milk contains human milk oligosaccharides (HMOs) and microRNAs (miRNAs), which are key bioactive components. HMOs are indigestible carbohydrates that impact infant growth and development. miRNAs are small, non-coding RNAs that regulate post-transcriptional gene expression. miRNAs a...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1463463/full |
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| author | Elizabeth A. Holzhausen Elizabeth A. Holzhausen William B. Patterson Benjamin H. Wong Sewan Kim Allison Kupsco Caitlin G. Howe Lars Bode Michael I. Goran Tanya L. Alderete |
| author_facet | Elizabeth A. Holzhausen Elizabeth A. Holzhausen William B. Patterson Benjamin H. Wong Sewan Kim Allison Kupsco Caitlin G. Howe Lars Bode Michael I. Goran Tanya L. Alderete |
| author_sort | Elizabeth A. Holzhausen |
| collection | DOAJ |
| description | IntroductionHuman milk contains human milk oligosaccharides (HMOs) and microRNAs (miRNAs), which are key bioactive components. HMOs are indigestible carbohydrates that impact infant growth and development. miRNAs are small, non-coding RNAs that regulate post-transcriptional gene expression. miRNAs are abundant in human milk and can be contained in extracellular vesicles (EVs). There is evidence that miRNAs are synthesized in the mammary epithelium and may influence mammary gland development and milk synthesis. However, the relationships between miRNAs and HMOs have yet to be fully characterized.MethodsThis study examined the associations between 210 human milk EV-miRNAs and 19 HMOs in a cohort of 98 Latina mothers. HMO measures included summary measures and concentrations of 19 HMOs. Relationships between EV-miRNAs and HMOs were examined using principal components analysis and associations between individual EV-miRNAs and HMOs were assessed.ResultsOverall patterns of EV-miRNA levels, summarized using principal components, were associated with HMO summary measures and concentrations. Levels of individual EV-miRNAs were associated with HMO summary measures and individual concentrations of 2’FL, 3FL, 3’SL, 6’SL, FLNH, LNFP I, and LNH.DiscussionResults from this study suggest that human milk EV-miRNAs are associated with the concentration of HMOs, which may have important effects on infant growth and development. |
| format | Article |
| id | doaj-art-6da7ea9fc6ba4b4c8d3be1f96603c3e3 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-6da7ea9fc6ba4b4c8d3be1f96603c3e32024-11-20T06:27:24ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14634631463463Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milkElizabeth A. Holzhausen0Elizabeth A. Holzhausen1William B. Patterson2Benjamin H. Wong3Sewan Kim4Allison Kupsco5Caitlin G. Howe6Lars Bode7Michael I. Goran8Tanya L. Alderete9Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United StatesDepartment of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United StatesUniversity of Colorado Anschutz Medical Campus, Aurora, CO, United StatesDepartment of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York, NY, United StatesDepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, United StatesDepartment of Pediatrics, Larson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CORE), Human Milk Institute (HMI), University of California, San Diego, San Diego, CA, United StatesDepartment of Pediatrics, Children’s Hospital Los Angeles, Los Angeles, CA, United StatesDepartment of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, United StatesIntroductionHuman milk contains human milk oligosaccharides (HMOs) and microRNAs (miRNAs), which are key bioactive components. HMOs are indigestible carbohydrates that impact infant growth and development. miRNAs are small, non-coding RNAs that regulate post-transcriptional gene expression. miRNAs are abundant in human milk and can be contained in extracellular vesicles (EVs). There is evidence that miRNAs are synthesized in the mammary epithelium and may influence mammary gland development and milk synthesis. However, the relationships between miRNAs and HMOs have yet to be fully characterized.MethodsThis study examined the associations between 210 human milk EV-miRNAs and 19 HMOs in a cohort of 98 Latina mothers. HMO measures included summary measures and concentrations of 19 HMOs. Relationships between EV-miRNAs and HMOs were examined using principal components analysis and associations between individual EV-miRNAs and HMOs were assessed.ResultsOverall patterns of EV-miRNA levels, summarized using principal components, were associated with HMO summary measures and concentrations. Levels of individual EV-miRNAs were associated with HMO summary measures and individual concentrations of 2’FL, 3FL, 3’SL, 6’SL, FLNH, LNFP I, and LNH.DiscussionResults from this study suggest that human milk EV-miRNAs are associated with the concentration of HMOs, which may have important effects on infant growth and development.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1463463/fullmicroRNAhuman milk (HM)human milk oligosaccharidesextracellular vesiclesEV-microRNAs |
| spellingShingle | Elizabeth A. Holzhausen Elizabeth A. Holzhausen William B. Patterson Benjamin H. Wong Sewan Kim Allison Kupsco Caitlin G. Howe Lars Bode Michael I. Goran Tanya L. Alderete Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk Frontiers in Immunology microRNA human milk (HM) human milk oligosaccharides extracellular vesicles EV-microRNAs |
| title | Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk |
| title_full | Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk |
| title_fullStr | Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk |
| title_full_unstemmed | Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk |
| title_short | Associations between human milk EV-miRNAs and oligosaccharide concentrations in human milk |
| title_sort | associations between human milk ev mirnas and oligosaccharide concentrations in human milk |
| topic | microRNA human milk (HM) human milk oligosaccharides extracellular vesicles EV-microRNAs |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1463463/full |
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