The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis
The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1 with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weigh...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/407284 |
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| author | Reza Farzinebrahimi Rosna Mat Taha Kamaludin Rashid Jamilah Syafawati Yaacob |
| author_facet | Reza Farzinebrahimi Rosna Mat Taha Kamaludin Rashid Jamilah Syafawati Yaacob |
| author_sort | Reza Farzinebrahimi |
| collection | DOAJ |
| description | The leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1 with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus. It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction from G. jasminoides plantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect against E. coli, P. aeruginosa, S. aureus, and B. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity against E. coli and B. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts. |
| format | Article |
| id | doaj-art-6cf38150da604a0a90e54872a742c71d |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-6cf38150da604a0a90e54872a742c71d2025-02-03T05:53:01ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/407284407284The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides EllisReza Farzinebrahimi0Rosna Mat Taha1Kamaludin Rashid2Jamilah Syafawati Yaacob3Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaBiology Division, Centre for Foundation Studies in Science Building, University of Malaya, 50603 Kuala Lumpur, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, MalaysiaThe leaf of Gardenia jasminoides Ellis was used as explants and was cultured on MS and WPM media supplemented with various concentrations of NAA, IAA, 2,4-D, IBA, TDZ, and Kn (0 to 5 mg L−1 with 0.5 increment). After six months, the higher percentage of callus (100%) and the best dry and fresh weight of callus were formed on WPM medium supplemented with 2,4-D and NAA (2.0-3.0 mg L−1) and this amount was decreased from (84%) to (69%) when this media supplemented with Kinetin and TDZ (1 mg L−1) respectively were used. Leaf segments cultured on WPM media added with Kn (1 mg L−1) and TDZ (2 mg L−1) yielded the least amount of callus. It was found that WPM media added with IAA (4.5–5.0 mg L−1) were optimum for root induction from G. jasminoides plantlets. Antibacterial screening of leaf extracts (in vivo) showed no inhibitory effect against E. coli, P. aeruginosa, S. aureus, and B. cereus, in contrast to callus extracts from leaf cultures supplemented with NAA, which showed inhibition activity against E. coli and B. cereus. The callus extracts from leaf cultures grown on both MS and WPM media showed higher antioxidant and superoxide dismutase activities than leaf extracts.http://dx.doi.org/10.1155/2014/407284 |
| spellingShingle | Reza Farzinebrahimi Rosna Mat Taha Kamaludin Rashid Jamilah Syafawati Yaacob The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis The Scientific World Journal |
| title | The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis |
| title_full | The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis |
| title_fullStr | The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis |
| title_full_unstemmed | The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis |
| title_short | The Effect of Various Media and Hormones via Suspension Culture on Secondary Metabolic Activities of (Cape Jasmine) Gardenia jasminoides Ellis |
| title_sort | effect of various media and hormones via suspension culture on secondary metabolic activities of cape jasmine gardenia jasminoides ellis |
| url | http://dx.doi.org/10.1155/2014/407284 |
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