The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance
Abstract One of the primary reasons for the failure of therapy in nasopharyngeal cancer (NPC) is radio resistance-related localized one, which may lead to tumor residuals or recurrences. Several studies have linked interleukin-10 (IL-10) to crucial functions in cancer development and response to the...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83423-2 |
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| author | Li-jun Wang Ying Zhao Yan-sheng Wang Lei Xing Yun-jing Duan Huan-huan Zang |
| author_facet | Li-jun Wang Ying Zhao Yan-sheng Wang Lei Xing Yun-jing Duan Huan-huan Zang |
| author_sort | Li-jun Wang |
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| description | Abstract One of the primary reasons for the failure of therapy in nasopharyngeal cancer (NPC) is radio resistance-related localized one, which may lead to tumor residuals or recurrences. Several studies have linked interleukin-10 (IL-10) to crucial functions in cancer development and response to therapy. Its function in NPC’s radio resistance is, however, not well understood. Enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR were utilized for confirming IL-10 expression in NPC cell lines. The prognostic significance of IL-10 was also assessed via Kaplan-Meier analysis. CNE2R, a radioresistant NPC cell line, expressed IL-10 at high levels, which were also shown to be considerably elevated in individuals with radioresistant NPC, as measured by ELISA. Moreover, the levels were also linked to poor clinical outcomes and prognosis in cancer cases. We also showed some evidence of a link between hypoxia-inducible factor 1-alpha (HIF-1 A) and serum IL-10 levels in NPC. Meanwhile, we find that IL-10 is up-regulated in CSC. IL-10 enhanced the self-renewal and tumorigenesis of nasopharyngeal CSC. In terms of mechanism, IL-10 enhances nasopharyngeal CSC self-renewal and tumorigenesis by activating STAT3 pathway. In NPC, IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance. |
| format | Article |
| id | doaj-art-6ce007a80611427b91c24993ca1a8548 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-6ce007a80611427b91c24993ca1a85482025-01-05T12:27:16ZengNature PortfolioScientific Reports2045-23222024-12-0114111010.1038/s41598-024-83423-2The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistanceLi-jun Wang0Ying Zhao1Yan-sheng Wang2Lei Xing3Yun-jing Duan4Huan-huan Zang5Department of Otolaryngology Head and Neck Surgery, Shijiazhuang People’s HospitalDepartment of Otolaryngology Head and Neck Surgery, Shijiazhuang People’s HospitalDepartment of Nuclear Medicine, General Hospital of Shenzhen UniversityDepartment of Otolaryngology Head and Neck Surgery, Shijiazhuang People’s HospitalDepartment of Otolaryngology Head and Neck Surgery, Shijiazhuang People’s HospitalDepartment of Otolaryngology Head and Neck Surgery, Shijiazhuang People’s HospitalAbstract One of the primary reasons for the failure of therapy in nasopharyngeal cancer (NPC) is radio resistance-related localized one, which may lead to tumor residuals or recurrences. Several studies have linked interleukin-10 (IL-10) to crucial functions in cancer development and response to therapy. Its function in NPC’s radio resistance is, however, not well understood. Enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR were utilized for confirming IL-10 expression in NPC cell lines. The prognostic significance of IL-10 was also assessed via Kaplan-Meier analysis. CNE2R, a radioresistant NPC cell line, expressed IL-10 at high levels, which were also shown to be considerably elevated in individuals with radioresistant NPC, as measured by ELISA. Moreover, the levels were also linked to poor clinical outcomes and prognosis in cancer cases. We also showed some evidence of a link between hypoxia-inducible factor 1-alpha (HIF-1 A) and serum IL-10 levels in NPC. Meanwhile, we find that IL-10 is up-regulated in CSC. IL-10 enhanced the self-renewal and tumorigenesis of nasopharyngeal CSC. In terms of mechanism, IL-10 enhances nasopharyngeal CSC self-renewal and tumorigenesis by activating STAT3 pathway. In NPC, IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance.https://doi.org/10.1038/s41598-024-83423-2Nasopharyngeal carcinomaInterleukin-10Radio resistanceCSCsSTAT3 |
| spellingShingle | Li-jun Wang Ying Zhao Yan-sheng Wang Lei Xing Yun-jing Duan Huan-huan Zang The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance Scientific Reports Nasopharyngeal carcinoma Interleukin-10 Radio resistance CSCs STAT3 |
| title | The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance |
| title_full | The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance |
| title_fullStr | The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance |
| title_full_unstemmed | The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance |
| title_short | The IL-10/STAT3 Axis Nasopharyngeal Carcinoma Cancer stem cell and radio resistance |
| title_sort | il 10 stat3 axis nasopharyngeal carcinoma cancer stem cell and radio resistance |
| topic | Nasopharyngeal carcinoma Interleukin-10 Radio resistance CSCs STAT3 |
| url | https://doi.org/10.1038/s41598-024-83423-2 |
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