Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)
Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization....
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612400326X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543992118870016 |
|---|---|
| author | Aistė Petruškevičiūtė Ugnė Šimuliūnaitė Catalina M. Polanco Barbara Rojas Simonas Kuras Beatričė Valatkaitė-Rakštienė Rimvydas Norvilas Akshay Kumar Vijaya Patricia Muñoz Urtė Neniškytė Artūras Jakubauskas Aurelijus Burokas Ivan Nalvarte Jose Inzunza Daniel Naumovas Mindaugas Stoškus Laimonas Griškevičius Daiva Baltriukienė Jonathan Arias |
| author_facet | Aistė Petruškevičiūtė Ugnė Šimuliūnaitė Catalina M. Polanco Barbara Rojas Simonas Kuras Beatričė Valatkaitė-Rakštienė Rimvydas Norvilas Akshay Kumar Vijaya Patricia Muñoz Urtė Neniškytė Artūras Jakubauskas Aurelijus Burokas Ivan Nalvarte Jose Inzunza Daniel Naumovas Mindaugas Stoškus Laimonas Griškevičius Daiva Baltriukienė Jonathan Arias |
| author_sort | Aistė Petruškevičiūtė |
| collection | DOAJ |
| description | Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone. |
| format | Article |
| id | doaj-art-6cd1289cd34c42669deb1fd756b25555 |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-6cd1289cd34c42669deb1fd756b255552025-01-13T04:18:40ZengElsevierStem Cell Research1873-50612025-02-0182103628Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)Aistė Petruškevičiūtė0Ugnė Šimuliūnaitė1Catalina M. Polanco2Barbara Rojas3Simonas Kuras4Beatričė Valatkaitė-Rakštienė5Rimvydas Norvilas6Akshay Kumar Vijaya7Patricia Muñoz8Urtė Neniškytė9Artūras Jakubauskas10Aurelijus Burokas11Ivan Nalvarte12Jose Inzunza13Daniel Naumovas14Mindaugas Stoškus15Laimonas Griškevičius16Daiva Baltriukienė17Jonathan Arias18VU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania; Doctoral Program of Biochemistry, Vilnius University Life Science Center, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania; Doctoral Program of Biology, Vilnius University Life Science Center, LithuaniaHematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania; Hematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaDoctoral Program of Biochemistry, Vilnius University Life Science Center, Lithuania; Department of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio Ave. 7, LT-10257 Vilnius, LithuaniaKarolinska Institutet, Department of Neurobiology, Care Sciences and Society, BioClinicum, Visionsgatan 4, Solna, Sweden; Karolinska Institutet Stem Cell Organoid (KISCO) Facility, Department of Laboratory Medicine, Alfred Nobels allé 8, Huddinge, SwedenVU LSC - EMBL Partnership Institute For Gene Editing Technologies – Laboratory of Targeted CRISPR-Cas Delivery into Mammalian Nervous System, LithuaniaHematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaDepartment of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio Ave. 7, LT-10257 Vilnius, LithuaniaKarolinska Institutet, Department of Neurobiology, Care Sciences and Society, BioClinicum, Visionsgatan 4, Solna, SwedenKarolinska Institutet Stem Cell Organoid (KISCO) Facility, Department of Laboratory Medicine, Alfred Nobels allé 8, Huddinge, Sweden; Karolinska Institutet, Department of Laboratory Medicine, Alfred Nobels allé 8, Huddinge, SwedenVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania; Doctoral Program of Biology, Vilnius University Life Science Center, Lithuania; Hematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaHematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaHematology, Oncology and Transfusion Medicine Center, Vilnius University Hospital Santaros Klinikos, Vilnius, LithuaniaDepartment of Biological Models, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saulėtekio Ave. 7, LT-10257 Vilnius, LithuaniaVU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania; Laboratory of Nuclease Enabled Cell Therapies, Lithuania; Karolinska Institutet Stem Cell Organoid (KISCO) Facility, Department of Laboratory Medicine, Alfred Nobels allé 8, Huddinge, Sweden; Karolinska Institutet, Department of Laboratory Medicine, Alfred Nobels allé 8, Huddinge, Sweden; Corresponding author at: VU LSC - EMBL Partnership Institute for Gene Editing Technologies, Saulėtekio avenue 7, Vilnius, Lithuania.Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone.http://www.sciencedirect.com/science/article/pii/S187350612400326XGenetically encoded reporterMARINACas9iPS cellsCRISPR |
| spellingShingle | Aistė Petruškevičiūtė Ugnė Šimuliūnaitė Catalina M. Polanco Barbara Rojas Simonas Kuras Beatričė Valatkaitė-Rakštienė Rimvydas Norvilas Akshay Kumar Vijaya Patricia Muñoz Urtė Neniškytė Artūras Jakubauskas Aurelijus Burokas Ivan Nalvarte Jose Inzunza Daniel Naumovas Mindaugas Stoškus Laimonas Griškevičius Daiva Baltriukienė Jonathan Arias Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) Stem Cell Research Genetically encoded reporter MARINA Cas9 iPS cells CRISPR |
| title | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) |
| title_full | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) |
| title_fullStr | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) |
| title_full_unstemmed | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) |
| title_short | Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2) |
| title_sort | generation of a genetically encoded voltage indicator marina reporter human ips cell line using cas9 vulsci002 a 2 |
| topic | Genetically encoded reporter MARINA Cas9 iPS cells CRISPR |
| url | http://www.sciencedirect.com/science/article/pii/S187350612400326X |
| work_keys_str_mv | AT aistepetruskeviciute generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT ugnesimuliunaite generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT catalinampolanco generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT barbararojas generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT simonaskuras generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT beatricevalatkaiterakstiene generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT rimvydasnorvilas generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT akshaykumarvijaya generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT patriciamunoz generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT urteneniskyte generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT arturasjakubauskas generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT aurelijusburokas generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT ivannalvarte generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT joseinzunza generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT danielnaumovas generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT mindaugasstoskus generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT laimonasgriskevicius generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT daivabaltriukiene generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 AT jonathanarias generationofageneticallyencodedvoltageindicatormarinareporterhumanipscelllineusingcas9vulsci002a2 |