Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model
Quercetin is a flavonol that is widely distributed in plants. Although quercetin has good antioxidant activity, the exact mechanism is still unclear in intestinal cell model. In this study, a 3D Caco-2 cell model was constructed. An oxidative damage model was then established. Compared with 2D cell...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624006534 |
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| author | Xiaofeng Liu Zhiyuan Zhou Xiaoqin Lu Hao Zhong Rongjun He Ziwei Feng Rongfa Guan |
| author_facet | Xiaofeng Liu Zhiyuan Zhou Xiaoqin Lu Hao Zhong Rongjun He Ziwei Feng Rongfa Guan |
| author_sort | Xiaofeng Liu |
| collection | DOAJ |
| description | Quercetin is a flavonol that is widely distributed in plants. Although quercetin has good antioxidant activity, the exact mechanism is still unclear in intestinal cell model. In this study, a 3D Caco-2 cell model was constructed. An oxidative damage model was then established. Compared with 2D cell models, 3D cell models are more similar to the in vivo microenvironment and more sensitive to external stimuli. Quercetin and its nanoliposomes significantly increased intracellular SOD, GSH-Px and CAT activity and the T-AOC, and reduced ROS, IL-8 and MDA levels to exert antioxidant effects (P < 0.05). Moreover, the antioxidant effect of the quercetin nanoliposomes was superior to that of quercetin. Quercetin nanoliposomes can regulate the oxidative genes HO-1, Keap1, and Nrf2, activating downstream antioxidant enzymes and upregulating GST, NQO1, and Keap1 protein expression. Quercetin nanoliposomes protect cells from oxidative stress through the Nrf2 pathway. |
| format | Article |
| id | doaj-art-6cc6eff8eb164301bc390239f1fe0279 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-6cc6eff8eb164301bc390239f1fe02792025-01-12T05:24:47ZengElsevierJournal of Functional Foods1756-46462025-01-01124106650Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell modelXiaofeng Liu0Zhiyuan Zhou1Xiaoqin Lu2Hao Zhong3Rongjun He4Ziwei Feng5Rongfa Guan6College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Huzhou 310014, China; Moganshan Institute ZJUT, Zhejiang, Hangzhou 313200, China; Corresponding author at: College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, China.Quercetin is a flavonol that is widely distributed in plants. Although quercetin has good antioxidant activity, the exact mechanism is still unclear in intestinal cell model. In this study, a 3D Caco-2 cell model was constructed. An oxidative damage model was then established. Compared with 2D cell models, 3D cell models are more similar to the in vivo microenvironment and more sensitive to external stimuli. Quercetin and its nanoliposomes significantly increased intracellular SOD, GSH-Px and CAT activity and the T-AOC, and reduced ROS, IL-8 and MDA levels to exert antioxidant effects (P < 0.05). Moreover, the antioxidant effect of the quercetin nanoliposomes was superior to that of quercetin. Quercetin nanoliposomes can regulate the oxidative genes HO-1, Keap1, and Nrf2, activating downstream antioxidant enzymes and upregulating GST, NQO1, and Keap1 protein expression. Quercetin nanoliposomes protect cells from oxidative stress through the Nrf2 pathway.http://www.sciencedirect.com/science/article/pii/S1756464624006534Three-dimensional modelQuercetin nanoliposomesOxidative damageProtection mechanismNrf2 pathway |
| spellingShingle | Xiaofeng Liu Zhiyuan Zhou Xiaoqin Lu Hao Zhong Rongjun He Ziwei Feng Rongfa Guan Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model Journal of Functional Foods Three-dimensional model Quercetin nanoliposomes Oxidative damage Protection mechanism Nrf2 pathway |
| title | Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model |
| title_full | Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model |
| title_fullStr | Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model |
| title_full_unstemmed | Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model |
| title_short | Protective mechanism of quercetin nanoliposomes on hydrogen peroxide-induced oxidative damage in 3D Caco-2 cell model |
| title_sort | protective mechanism of quercetin nanoliposomes on hydrogen peroxide induced oxidative damage in 3d caco 2 cell model |
| topic | Three-dimensional model Quercetin nanoliposomes Oxidative damage Protection mechanism Nrf2 pathway |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624006534 |
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