Enzymatic Hydrolysis of Pretreated Miscanthus Biomass
Forests being mostly non-renewable, miscanthus seems to be a promising alternative energy resource. This study investigates the enzymatic hydrolysis of pretreated miscanthus biomass. The research featured Miscanthus sinensis Strictus biomass. The methods included raw material pretreatment, enzymatic...
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| Format: | Article |
| Language: | English |
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Kemerovo State University
2022-06-01
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| Series: | Техника и технология пищевых производств |
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| Online Access: | https://fptt.ru/en/issues/20365/20375/ |
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| _version_ | 1846146089858629632 |
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| author | Olga B. Kalashnikova Ekaterina A. Budenkova Elena V. Ulrikh Evgeny G. Chupakhin Olga V. Kriger Yakov A. Masiutin Maria A. Smaga Yulia S. Basova |
| author_facet | Olga B. Kalashnikova Ekaterina A. Budenkova Elena V. Ulrikh Evgeny G. Chupakhin Olga V. Kriger Yakov A. Masiutin Maria A. Smaga Yulia S. Basova |
| author_sort | Olga B. Kalashnikova |
| collection | DOAJ |
| description | Forests being mostly non-renewable, miscanthus seems to be a promising alternative energy resource. This study investigates the enzymatic hydrolysis of pretreated miscanthus biomass.
The research featured Miscanthus sinensis Strictus biomass. The methods included raw material pretreatment, enzymatic hydrolysis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) of monosaccharide residues, and nuclear magnetic resonance (1H NMR) spectrometry of non-hydrolyzed lignin.
A hammer mill with a particle size of 0.2–0.3 mm proved to be effective for preliminary mechanical processing of miscanthus. This type of treatment provided the maximal yield of carbohydrate-containing biomass (62.33 ± 1.87%). The optimal multi-enzyme composition included Cellulase Ultra obtained from Trichoderma reeseii, xylanase obtained from Thermomyces lanuginosus, and β-gluconase obtained from Myceliophtorafergusii. The fermentation temperature was 50 ± 1°C (72 ± 1 h). At these parameters, the conversion of holocellulose of miscanthus biomass was 96.0 ± 4.8%, and the yield of reducing substances was 97.00 ± 4.85%.
The article introduces the optimal enzymatic hydrolysis parameters for pretreating Miscanthus sinensis Strictus biomass as a source of carbohydrate-containing substrates and describes their subsequent use for bacterial cellulose biosynthesis. The carbohydrate composition of hydrolysates included residual lignin, such polysaccharides as glucan and xylan, and various monosaccharides, namely arabinose, xylose, galactose, glucose, and uronic acids. Hydrolyzed miscanthus proved to be an effective renewable and environmentally friendly biodiesel. |
| format | Article |
| id | doaj-art-6c6ffb72a5294aeb967e09476ffa0ace |
| institution | Kabale University |
| issn | 2074-9414 2313-1748 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Kemerovo State University |
| record_format | Article |
| series | Техника и технология пищевых производств |
| spelling | doaj-art-6c6ffb72a5294aeb967e09476ffa0ace2024-12-02T04:42:56ZengKemerovo State UniversityТехника и технология пищевых производств2074-94142313-17482022-06-0152225427010.21603/2074-9414-2022-2-2361Enzymatic Hydrolysis of Pretreated Miscanthus BiomassOlga B. Kalashnikova0https://orcid.org/0000-0002-6105-8631Ekaterina A. Budenkova1https://orcid.org/0000-0003-4854-5459Elena V. Ulrikh2https://orcid.org/0000-0003-4107-7277Evgeny G. Chupakhin3https://orcid.org/0000-0001-5284-4748Olga V. Kriger4https://orcid.org/0000-0002-1489-0716Yakov A. Masiutin5https://orcid.org/0000-0003-4347-3425Maria A. Smaga6https://orcid.org/0000-0001-5304-5761Yulia S. Basova7https://orcid.org/0000-0001-5361-5971Immanuel Kant Baltic Federal University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaKaliningrad State Technical University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaImmanuel Kant Baltic Federal University, Kaliningrad, RussiaForests being mostly non-renewable, miscanthus seems to be a promising alternative energy resource. This study investigates the enzymatic hydrolysis of pretreated miscanthus biomass. The research featured Miscanthus sinensis Strictus biomass. The methods included raw material pretreatment, enzymatic hydrolysis, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) of monosaccharide residues, and nuclear magnetic resonance (1H NMR) spectrometry of non-hydrolyzed lignin. A hammer mill with a particle size of 0.2–0.3 mm proved to be effective for preliminary mechanical processing of miscanthus. This type of treatment provided the maximal yield of carbohydrate-containing biomass (62.33 ± 1.87%). The optimal multi-enzyme composition included Cellulase Ultra obtained from Trichoderma reeseii, xylanase obtained from Thermomyces lanuginosus, and β-gluconase obtained from Myceliophtorafergusii. The fermentation temperature was 50 ± 1°C (72 ± 1 h). At these parameters, the conversion of holocellulose of miscanthus biomass was 96.0 ± 4.8%, and the yield of reducing substances was 97.00 ± 4.85%. The article introduces the optimal enzymatic hydrolysis parameters for pretreating Miscanthus sinensis Strictus biomass as a source of carbohydrate-containing substrates and describes their subsequent use for bacterial cellulose biosynthesis. The carbohydrate composition of hydrolysates included residual lignin, such polysaccharides as glucan and xylan, and various monosaccharides, namely arabinose, xylose, galactose, glucose, and uronic acids. Hydrolyzed miscanthus proved to be an effective renewable and environmentally friendly biodiesel.https://fptt.ru/en/issues/20365/20375/miscanthusbiofuelenzymatic hydrolysis pretreatmentreducing sugarsnon-hydrolysed lignincellulose |
| spellingShingle | Olga B. Kalashnikova Ekaterina A. Budenkova Elena V. Ulrikh Evgeny G. Chupakhin Olga V. Kriger Yakov A. Masiutin Maria A. Smaga Yulia S. Basova Enzymatic Hydrolysis of Pretreated Miscanthus Biomass Техника и технология пищевых производств miscanthus biofuel enzymatic hydrolysis pretreatment reducing sugars non-hydrolysed lignin cellulose |
| title | Enzymatic Hydrolysis of Pretreated Miscanthus Biomass |
| title_full | Enzymatic Hydrolysis of Pretreated Miscanthus Biomass |
| title_fullStr | Enzymatic Hydrolysis of Pretreated Miscanthus Biomass |
| title_full_unstemmed | Enzymatic Hydrolysis of Pretreated Miscanthus Biomass |
| title_short | Enzymatic Hydrolysis of Pretreated Miscanthus Biomass |
| title_sort | enzymatic hydrolysis of pretreated miscanthus biomass |
| topic | miscanthus biofuel enzymatic hydrolysis pretreatment reducing sugars non-hydrolysed lignin cellulose |
| url | https://fptt.ru/en/issues/20365/20375/ |
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