Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV
Banana bunchy top disease is caused by banana bunchy top virus (BBTV). BBTV is transmitted locally by aphids (Pentalonia spp.), but the long-distance spread is through the movement of infected planting materials. This study investigated potential alternative hosts of BBTV in ornamental Musa and rela...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1467331/full |
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| author | Nicole Angelee P. Mendoza Jay-Vee S. Mendoza John E. Thomas Fe M. Dela Cueva |
| author_facet | Nicole Angelee P. Mendoza Jay-Vee S. Mendoza John E. Thomas Fe M. Dela Cueva |
| author_sort | Nicole Angelee P. Mendoza |
| collection | DOAJ |
| description | Banana bunchy top disease is caused by banana bunchy top virus (BBTV). BBTV is transmitted locally by aphids (Pentalonia spp.), but the long-distance spread is through the movement of infected planting materials. This study investigated potential alternative hosts of BBTV in ornamental Musa and related species in the Zingiberales in the Philippines. Artificial inoculation of BBTV, molecular detection and transmission assay were used to evaluate 15 plant test species. The potential for seed transmission of BBTV through Canna indica seeds was also investigated. Seed samples were validated and quantified for BBTV presence using molecular tools, and then grown for transmission assay. Typical symptoms of BBTV in bananas, including dark green streak on the midrib and petiole and rosetting were observed on inoculated Musa coccinea (banana blossom), M. velutina (velutina), M laterita. (bronze banana) and Canna indica (Bandera Espanola). PCR assays confirmed BBTV infection in these symptomatic test plants, as well as in Curcuma longa (turmeric) which exhibited large chlorotic blotches on the leaf. BBTV was detected from both seeds and germinated seedlings of artificially inoculated and field-collected C. indica samples. This study identified M. laterita as a new host of BBTV. The susceptibility to BBTV of M. coccinea, M. velutina, C. indica, and C. longa was also confirmed. The study also provided the first evidence of seed transmission of BBTV. C indica is an ornamental plant popularly used for landscaping in the Philippines and seeds were shown to be an efficient mode of transmission of the virus with rates up to 34%. The discovery of natural infection in ornamental plants and seeds poses a risk to the banana industry and responsible propagation and appropriate quarantine protocols must be implemented. |
| format | Article |
| id | doaj-art-6c47c2b2f121447bb7c9f98435b5bd0d |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-6c47c2b2f121447bb7c9f98435b5bd0d2024-11-27T17:51:11ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14673311467331Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTVNicole Angelee P. Mendoza0Jay-Vee S. Mendoza1John E. Thomas2Fe M. Dela Cueva3National Crop Protection Center, College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Laguna, PhilippinesPlant Pathology Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Laguna, PhilippinesQueensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, AustraliaPlant Pathology Laboratory, Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, Los Baños, Laguna, PhilippinesBanana bunchy top disease is caused by banana bunchy top virus (BBTV). BBTV is transmitted locally by aphids (Pentalonia spp.), but the long-distance spread is through the movement of infected planting materials. This study investigated potential alternative hosts of BBTV in ornamental Musa and related species in the Zingiberales in the Philippines. Artificial inoculation of BBTV, molecular detection and transmission assay were used to evaluate 15 plant test species. The potential for seed transmission of BBTV through Canna indica seeds was also investigated. Seed samples were validated and quantified for BBTV presence using molecular tools, and then grown for transmission assay. Typical symptoms of BBTV in bananas, including dark green streak on the midrib and petiole and rosetting were observed on inoculated Musa coccinea (banana blossom), M. velutina (velutina), M laterita. (bronze banana) and Canna indica (Bandera Espanola). PCR assays confirmed BBTV infection in these symptomatic test plants, as well as in Curcuma longa (turmeric) which exhibited large chlorotic blotches on the leaf. BBTV was detected from both seeds and germinated seedlings of artificially inoculated and field-collected C. indica samples. This study identified M. laterita as a new host of BBTV. The susceptibility to BBTV of M. coccinea, M. velutina, C. indica, and C. longa was also confirmed. The study also provided the first evidence of seed transmission of BBTV. C indica is an ornamental plant popularly used for landscaping in the Philippines and seeds were shown to be an efficient mode of transmission of the virus with rates up to 34%. The discovery of natural infection in ornamental plants and seeds poses a risk to the banana industry and responsible propagation and appropriate quarantine protocols must be implemented.https://www.frontiersin.org/articles/10.3389/fpls.2024.1467331/fullalternative hostbanana bunchy top virusornamentalsPentaloniaseed transmission |
| spellingShingle | Nicole Angelee P. Mendoza Jay-Vee S. Mendoza John E. Thomas Fe M. Dela Cueva Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV Frontiers in Plant Science alternative host banana bunchy top virus ornamentals Pentalonia seed transmission |
| title | Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV |
| title_full | Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV |
| title_fullStr | Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV |
| title_full_unstemmed | Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV |
| title_short | Alternative hosts of banana bunchy top virus in the Philippines and the first evidence of seed transmission of BBTV |
| title_sort | alternative hosts of banana bunchy top virus in the philippines and the first evidence of seed transmission of bbtv |
| topic | alternative host banana bunchy top virus ornamentals Pentalonia seed transmission |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1467331/full |
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