Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5
In this study, a high-yield aminopeptidase producing strain, Bacillus subtilis LBJ4-5, was isolated from Pixian Douban, and its safety was evaluated through hemolysis and antibiotic sensitivity tests. Additionally, Illumina second-generation sequencing and PacBio third-generation high-throughput seq...
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The editorial department of Science and Technology of Food Industry
2024-12-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030345 |
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| author | Baosong GUO Yujiao ZHANG Yiwei DAI Yingxi CHEN Liang DONG Sufang ZHANG |
| author_facet | Baosong GUO Yujiao ZHANG Yiwei DAI Yingxi CHEN Liang DONG Sufang ZHANG |
| author_sort | Baosong GUO |
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| description | In this study, a high-yield aminopeptidase producing strain, Bacillus subtilis LBJ4-5, was isolated from Pixian Douban, and its safety was evaluated through hemolysis and antibiotic sensitivity tests. Additionally, Illumina second-generation sequencing and PacBio third-generation high-throughput sequencing were employed for the whole-genome sequencing of B. subtilis LBJ4-5 to characterize its genomic features. Gene prediction and functional annotation were carried out using databases such as COG, GO, KEGG, CAZY, CARD and VFDB. Furthermore, the aminopeptidase production capacity and safety of the strain were evaluated.The results showed that after 48 hours of shake flask cultivation, the aminopeptidase activity in the fermentation supernatant of B. subtilis LBJ4-5 reached 179.23 U/mL. The strain showed no hemolytic activity and was highly sensitive (diameter≥20 mm) to 10 antibiotics, including cefazolin, vancomycin, penicillin G, and cefadroxil，indicating a favorable safety profile.The genome of B. subtilis LBJ4-5 consisted of a circular closed DNA and a circular plasmid, with a size of 4053926 bp and a GC content of 43.66%. A total of 4285 protein-coding genes, 85 tRNA genes, 33 rRNA genes, and 119 sRNAs were predicted. Functional gene annotations in COG, GO, KEGG, CAZY, CARD, and VFDB databases identified 3386, 2581, 2552, 144, 256 and 401 functional genes, respectively. Further analysis indicated that the genome of B. subtilis LBJ4-5 contains 31 aminopeptidase-encoding genes. Additionally, the prediction of virulence and antibiotic resistance genes further confirmed the safety of the B. subtilis LBJ4-5 strain. In conclusion, B. subtilis LBJ4-5 can be used as a fermentation starter for the processing of fermented foods. |
| format | Article |
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| institution | Kabale University |
| issn | 1002-0306 |
| language | zho |
| publishDate | 2024-12-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj-art-6c33d4ea74a64b30a6ad2df08b7875132024-12-06T03:28:22ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062024-12-014524324110.13386/j.issn1002-0306.20240303452024030345-24Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5Baosong GUO0Yujiao ZHANG1Yiwei DAI2Yingxi CHEN3Liang DONG4Sufang ZHANG5Collaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaCollaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaCollaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaCollaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaCollaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaCollaborative Innovation Center of Seafood Deep Processing, National Engineering Research Center of Seafood,SKL of Marine Food Processing & Safety Control, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaIn this study, a high-yield aminopeptidase producing strain, Bacillus subtilis LBJ4-5, was isolated from Pixian Douban, and its safety was evaluated through hemolysis and antibiotic sensitivity tests. Additionally, Illumina second-generation sequencing and PacBio third-generation high-throughput sequencing were employed for the whole-genome sequencing of B. subtilis LBJ4-5 to characterize its genomic features. Gene prediction and functional annotation were carried out using databases such as COG, GO, KEGG, CAZY, CARD and VFDB. Furthermore, the aminopeptidase production capacity and safety of the strain were evaluated.The results showed that after 48 hours of shake flask cultivation, the aminopeptidase activity in the fermentation supernatant of B. subtilis LBJ4-5 reached 179.23 U/mL. The strain showed no hemolytic activity and was highly sensitive (diameter≥20 mm) to 10 antibiotics, including cefazolin, vancomycin, penicillin G, and cefadroxil，indicating a favorable safety profile.The genome of B. subtilis LBJ4-5 consisted of a circular closed DNA and a circular plasmid, with a size of 4053926 bp and a GC content of 43.66%. A total of 4285 protein-coding genes, 85 tRNA genes, 33 rRNA genes, and 119 sRNAs were predicted. Functional gene annotations in COG, GO, KEGG, CAZY, CARD, and VFDB databases identified 3386, 2581, 2552, 144, 256 and 401 functional genes, respectively. Further analysis indicated that the genome of B. subtilis LBJ4-5 contains 31 aminopeptidase-encoding genes. Additionally, the prediction of virulence and antibiotic resistance genes further confirmed the safety of the B. subtilis LBJ4-5 strain. In conclusion, B. subtilis LBJ4-5 can be used as a fermentation starter for the processing of fermented foods.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030345bacillus subtiliswhole genome sequencingaminopeptidasegene functional annotationpixian douban |
| spellingShingle | Baosong GUO Yujiao ZHANG Yiwei DAI Yingxi CHEN Liang DONG Sufang ZHANG Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 Shipin gongye ke-ji bacillus subtilis whole genome sequencing aminopeptidase gene functional annotation pixian douban |
| title | Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 |
| title_full | Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 |
| title_fullStr | Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 |
| title_full_unstemmed | Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 |
| title_short | Whole-genome Sequencing and Sequence Analysis of an Aminopeptidase-producing Bacillus subtilis LBJ4-5 |
| title_sort | whole genome sequencing and sequence analysis of an aminopeptidase producing bacillus subtilis lbj4 5 |
| topic | bacillus subtilis whole genome sequencing aminopeptidase gene functional annotation pixian douban |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024030345 |
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